Glass surfaces with well defined surface morphologies have been prepared using photolithography to study the effect of surface morphology on cell adhesion and function. Using a transformed recombinant cell-line, AtT-20, as a model of shear sensitive cell, we have shown that cell-substratum adhesion strength is enhanced using a surface with uniform grooves without any loss in cellular function. Furthermore, using primary hepatocytes as a model for a cell whose function is sensitive to its shape, we have shown that surface morphology can modulate cell shape as well as its function.
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The authors wish to acknowledge the laboratory of D. E. Ingber and J. Vacanti at the Children’s Hospital in Boston for help in the procurement of primary rat hepotocytes and M. J. Cima at the Material Science and Engineering Department at MIT and D. Mooney and D.E. Ingber at Children’s hospital for helpful discussions. The authors also wish to acknowledge financial support from the NSF Engineering Research Center Initiative: ECD 8803014. R. Singhvi wishes to acknowledge a fellowship from the Merck Manufacturing Division of Merck and Company.
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Singhvi, R., Stephanopoulos, G.N. & WANG, D.I.C. Effect of Substratum Morphology on Animal Cell Adhesion and Behavior. MRS Online Proceedings Library 252, 237–245 (1991). https://doi.org/10.1557/PROC-252-237