The Use of Electrospun Polycaprolactone as a Dermal Scaffold for Skin Tissue Engineering

Abstract

Rapid healing of acute and chronic skin defects is an important objective. In the present work, we report on the design and feasibility of a co-culture system for fibroblasts and keratinocytes by using electrospun polycaprolactone (PCL) scaffolds. Specifically, we quantified the effect of scaffold fiber diameter on keratinocyte attachment, proliferation and differentiation along with collagen secretion by fibroblasts post vacuum seeding with fibroblasts at various depths. The results show that fibroblasts secrete more collagen and keratinocytes differentiate more on 400 nm scaffolds than on 1000 nm scaffolds. Also, fibroblasts co-cultured with keratinocytes provide increased collagen secretion and keratinocyte differentiation. These results suggest that the fiber architecture can be a useful parameter in skin tissue engineering.

This is a preview of subscription content, access via your institution.

References

  1. 1

    C. W. Lan, F. F. Wang and Y. J. Wang, J Biomed Mater Res A 66, 38 (2003).

    Article  Google Scholar 

  2. 2

    W. J. Li, C. T. Laurencin, E. J. Caterson, R. S. Tuan and F. K. Ko, J Biomed Mater Res 60, 613 (2002).

    CAS  Article  Google Scholar 

  3. 3

    E. D. Boland, G. E. Wnek, D. G. Simpson, K. J. Pawlowski and G. L. Bowlin J. Macromol. Sci. Pure Appl. Chem. A38, 1231 (2001).

    CAS  Article  Google Scholar 

  4. 4

    R. Murugan and S. Ramakrishna, Tissue Eng 12, 435 (2006).

    CAS  Article  Google Scholar 

  5. 5

    M. D. Basson, I. M. Modlin, G. Turowski and J. A. Madri, Eur J Gastroenterol Hepatol 5, 21 (1993).

    Google Scholar 

  6. 6

    H. Carsin, P. Ainaud, H. Le Bever, J. Rives, A. Lakhel, J. Stephanazzi, F. Lambert and J. Perrot, Burns 26, 379 (2000).

    CAS  Article  Google Scholar 

  7. 7

    T. J. Phillips and B. A. Gilchrest, Epithelial Cell Biol 1, 39 (1992).

    CAS  Google Scholar 

  8. 8

    F. Rippke, V. Schreiner and H. J. Schwanitz, Am J Clin Dermatol 3, 261 (2002).

    Article  Google Scholar 

  9. 9

    N. Ojeh, J. Frame and H. Navsaria, Tissue Eng 7, 457 (2001).

    CAS  Article  Google Scholar 

  10. 10

    A. El-Ghalbzouri, E.N. Lamme, C. van Blitterswijk, J. Koopman and M. Ponec, Biomaterials 25, 2987 (2004).

    CAS  Article  Google Scholar 

  11. 11

    A. F. Black, C. Bouez, E. Perrier, K. Schlotmann, F. Chapuis, Damour, O, Tissue Eng 11, 723 (2005).

    CAS  Article  Google Scholar 

  12. 12

    A. G. Coombes, E. Verderio, B. Shaw, X. Li, M. Griffin and S. Downes, Biomaterials 23, 2113 (2002).

    CAS  Article  Google Scholar 

  13. 13

    A. D. Schwope, D. L. Wise, K. W. Sell, W. A. Skornik and D. P. Dressler, D. P. Trans Am Soc Artif Intern Organs 20A, 103 (1974).

    CAS  Google Scholar 

  14. 14

    E. Bell, H. P. Ehrlich, D. J. Buttle and T. Nakatsuji. Science 211, 1052 (1981).

    CAS  Article  Google Scholar 

  15. 15

    N. Parenteau, Cambridge, (Cambridge University Press, U.K. 1994) p45.

    Google Scholar 

  16. 16

    M. Chen, P. K. Patra, S. B. Warner and S. Bhowmick, Biophys. Rev. Lett 1, 189 (2006).

    CAS  Article  Google Scholar 

  17. 17

    M. Chen, H. Michaud and S. Bhowmick, J Biomech Eng 131, 074521.1 (2009).

    Google Scholar 

  18. 18

    M. Chen, P. K. Patra, S. B. Warner and S. Bhowmick, Tissue Eng 13, 579 (2007)

    CAS  Article  Google Scholar 

  19. 19

    M. Chen, P. K. Patra, M. L. Lovett, D. L. Kaplan and S. Bhowmick, J Tissue Eng Regen Med 3, 269 (2009).

    CAS  Article  Google Scholar 

  20. 20

    I. J. Lim, T.T. Phan, B. H. Bay, R. Qi, H. Huynh and W. T. Tan, S. T. Lee and M. T. Longaker, Am J Physiol Cell Physiol 283, C212 (2002).

    CAS  Article  Google Scholar 

  21. 21

    J. G. Rheinwald, H. Green, Cell 6, 331 (1975).

    CAS  Article  Google Scholar 

  22. 22

    Y. Kuroyanagi, M. Kenmochi, S. Ishihara, A. Takeda, A. Shiraishi, N. Ootake, E. Uchinuma, K. Torikai, and N. Shioya, Ann Plast Surg 31, 340 (1993)

    CAS  Article  Google Scholar 

  23. 23

    J. S. Rubin, H. Osada, P. W. Finch, W. G. Taylor, S. Rudikoff and S. A. Aaronson, Proc Natl Acad Sci U S A 86, 802(1989)

    CAS  Article  Google Scholar 

  24. 24

    C. Marchese, J. Rubin, D. Ron, A. Faggioni, M. R. Torrisi, A. Messina, A, L. Frati, and S. A. Aaronson, J Cell Physiol 144, 326(1990).

    CAS  Article  Google Scholar 

  25. 25

    D. Y. Lee, J. H. Lee, E. S. Lee, K. H. Cho and J. M. Yang, Arch Dermatol Res 294, 444 (2003).

    Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Chen, M., Chopra, M. & Bhowmick, S. The Use of Electrospun Polycaprolactone as a Dermal Scaffold for Skin Tissue Engineering. MRS Online Proceedings Library 1235, 504 (2009). https://doi.org/10.1557/PROC-1235-RR05-04

Download citation