A Self-assembled, drug-deliverable Nanomaterial for Cartilage Tissue Engineering

Abstract

The current clinical treatment of cartilage defects involves autologous chondrocyte implantation into cartilage defect sites. However, one of the complications associated with this method is the lack of bonding between the implanted materials and natural tissue. Helical rosette nanotubes (HRNs) are novel biomimetic self-assembled supramolecular structures whose basic building blocks are DNA base-pairs. HRNs are similar in size to collagen in cartilage. Moreover, previous studies have shown that HRNs are biocompatible and increase the adhesion of numerous cells compared to other commonly used cartilage implant materials (like hydrogels and Ti). In addition, HRNs can solidify into a viscous gel at body temperatures under short periods of time. Thus, it is hoped that HRNs can serve as a novel in situ tissue implant to improve cartilage cell adhesion and functions. In this study, in order to heal cartilage rupture and regenerate cartilage during possible implantation, the mechanical properties of select hydrogel/HRN composites were tested. In addition, electro-spinning was used to generate three-dimensional, implantable, composite fibers encapsulated with chondrocytes and fibroblast-like type-B synoviocytes (SFB cells, a type of mesenchymal stem cell). Importantly, results showed that drug-delivered HRNs enhanced hydrogel adhesive strength and created a scaffold with nanometer-rough surface structures pertinent for cartilage regeneration. In this manner, this study provided an alternative cartilage regenerative material which relies on nanotechnology that can be injected as a liquid, solidify at body temperatures under short periods of time, have suitable mechanical properties to collagen, and promote cell functions.

This is a preview of subscription content, access via your institution.

References

  1. 1.

    RS Tuan, G Boland, R. Tuli, Adult mesenchymal stem cells and cell-based tissue engineering. Arthritis Research and Therapy, 2002, 5, 32–45.

    Article  Google Scholar 

  2. 2.

    Newswire PR, Milestone Scientific Signs Agreement With Carticept Medical to Collaborate on CompuFlo(TM) Injection System for Treatment of Arthritic Joints, 2007.

  3. 3.

    R Langer, JP Vacanti . Tissue engineering. Science 1993; 260: 920–926.

    CAS  Google Scholar 

  4. 4.

    JS Temenoff, AG. Mikos Review: tissue engineering for regeneration of articular cartilage. Biomaterials 2000; 21: 431–440.

    CAS  Article  Google Scholar 

  5. 5.

    M Brittberg, A Lindahl, A Nilsson, C Ohlsson, O Isaksson, L. Peterson Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 1994; 331: 889–895.

    CAS  Article  Google Scholar 

  6. 6.

    WC Puelacher, D Mooney, R Langer, J Upton, JP Vacanti, CA. Vacanti Design of nasoseptal cartilage replacements synthesized from biodegradable polymers and chondrocytea Biomaterials 1994; 15:774–778.

    CAS  Article  Google Scholar 

  7. 7.

    LE Freed, JC Marquis, A Nohria, J Emmanual, AG Mikos, R. Langer Neocartilage formation IN VITRO and IN VIVO using cells cultured on synthetic biodegradable polymers. J Biomed Mater Res 1993; 27:11–23.

    CAS  Article  Google Scholar 

  8. 8.

    DA Grande, C Halberstadt, G Naughton, R Schwartz, R. Manji, Evaluation of matrix scaffolds for tissue engineering of articular cartilage grafts. J Biomed Mater Res 1997; 34: 211–220.

    CAS  Article  Google Scholar 

  9. 9.

    S Nehrer, HA Breinan, A Ramappa, S Shortkroff, G Young, T Minas, CB Sledge, IV Yannas, Spector M.Canine chondrocytes seeded in type I and type II collagen implants investigated in vitro. J Biomed Mater Res 1997; 38: 95–104.

    CAS  Article  Google Scholar 

  10. 10.

    P Brun, G Abatangelo, M Radice, V Zacchi, D Guidolin, D Daga Gordini, R Cortivo . Chondrocyte aggregation and reorganization into three-dimensional scaffolds. J Biomed Mater Res 1999; 46: 337–346.

    CAS  Article  Google Scholar 

  11. 11.

    AL Chun, J G Moralez, H Fenniri, TJ. Webster Helical rosette nanotubes: a biomimetic coating for orthopedics? Biomaterials 2005; 26: 7304–7309.

  12. 12.

    AL Chun, J G Moralez, H Fenniri, TJ. Webster Helical rosette nanotubes: A more effective orthopaedic implant material. Nanotechnology 2004; 15: s234–s239.

    CAS  Article  Google Scholar 

  13. 13.

    H Fenniri, P mathivanan, KL Vidale, DM Sherman, K Hallenga, KV wood and JG. Stowell Helical rosette nanotubes: design, self-assembly and characterization. J.Am. Chem.Soc. 2001, 123, 3854–3855.

    CAS  Article  Google Scholar 

  14. 14.

    MB Eslaminejad, H Mirzadeh, Y Mohamadi, A. Nickmahzar Bone differentiation of marrow-derived mesenchymal stem cells using beta-tricalcium phosphate-alginate-gelatin hybrid scaffolds. J Tissue Eng Regen Med. 2007 Nov-Dec; 1(6):417–424.

    CAS  Article  Google Scholar 

  15. 15.

    T Kurth, E Hedbom, N Shintani, M Sugimoto, FH Chen, M Haspl, S Martinovic, EB. Hunziker Chondrogenic potential of human synovial mesenchymal stem cells in alginate. Osteoarthritis Cartilage. 2007 Oct; 15(10):1178–1189.

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Yupeng Chen.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Chen, Y., Fenniri, H. & Thomas, J.W. A Self-assembled, drug-deliverable Nanomaterial for Cartilage Tissue Engineering. MRS Online Proceedings Library 1138, 1002 (2008). https://doi.org/10.1557/PROC-1138-FF10-02

Download citation