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Nanoscale “Curtain Rods”: High-Throughput Tools For Studying DNA-Protein Interactions

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Abstract

In this work, we combine nanoscale engineering with single-molecule biology to probe the biochemical interactions between individual proteins and DNA. This approach, a vast improvement over previous methods, constructs a platform to observe thousands of protein-DNA interactions in real time with unprecedented detail. A key challenge in these experiments involves collecting enough statistically relevant data in order to analyze reactions which are designed to be probed individually. “DNA curtains” are formed by flowing the DNA tethered to a lipid bilayer across nanopatterned barriers, facilitating massively parallel data acquisition.

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Authors and Affiliations

  1. Department of Applied Physics and Applied Mathematics, Columbia University, 500 W. 120thSt, 10027, New York, NY, U.S.A.

    A. Fazio Teresa & J. Wind Shalom

  2. Department of Biochemistry and Molecular Biophysics, Columbia University, 530 W. 168thSt, 10028, New York, NY, USA

    Mari-Liis Visnapuu & C. Greene Eric

Authors
  1. A. Fazio Teresa
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  2. Mari-Liis Visnapuu
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  3. J. Wind Shalom
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  4. C. Greene Eric
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Teresa, A.F., Visnapuu, ML., Shalom, J.W. et al. Nanoscale “Curtain Rods”: High-Throughput Tools For Studying DNA-Protein Interactions. MRS Online Proceedings Library 1138, 803 (2008). https://doi.org/10.1557/PROC-1138-FF08-03

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  • DOI: https://doi.org/10.1557/PROC-1138-FF08-03

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