In this work, we combine nanoscale engineering with single-molecule biology to probe the biochemical interactions between individual proteins and DNA. This approach, a vast improvement over previous methods, constructs a platform to observe thousands of protein-DNA interactions in real time with unprecedented detail. A key challenge in these experiments involves collecting enough statistically relevant data in order to analyze reactions which are designed to be probed individually. “DNA curtains” are formed by flowing the DNA tethered to a lipid bilayer across nanopatterned barriers, facilitating massively parallel data acquisition.
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Teresa, A.F., Visnapuu, ML., Shalom, J.W. et al. Nanoscale “Curtain Rods”: High-Throughput Tools For Studying DNA-Protein Interactions. MRS Online Proceedings Library 1138, 803 (2008). https://doi.org/10.1557/PROC-1138-FF08-03