Nanoparticles are of interest in many applications since their decreased size may give them properties that are very different from bulk material. Often nanoparticle properties such as size (diameter) and size distribution are evaluated using transmission electron microscopy (TEM). These parameters, size and size distribution, can be more easily obtained from digitized TEM images by mapping particle signal to black and background pixel to white in a process known as thresholding then performing an algorithm known as a particle analysis. The goal of this study was to compare the ability of several popular thresholding algorithms to segment TEM images. Performance of the thresholding algorithms was evaluated through qualitative and quantitative measures. Results show that the choice of a thresholding algorithm will strongly affect the results obtained from particle analysis.
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Sadowski, T.E., Broadbridge, C.C. & Daponte, J. Comparison of Common Segmentation Techniques Applied to Transmission Electron Microscopy Images. MRS Online Proceedings Library 982, 704 (2006). https://doi.org/10.1557/PROC-0982-KK07-04