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Electron-Beam Directed Layer-by-Layer Assembly of Dendrimer Scaffold for Biomolecule Patterning

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Abstract

A method for patterning biomolecules using electron beam (e-beam) lithography has been developed. A non-biofouling poly(ethylene glycol) terminated self-assembled monolayer (SAM) was ablated by e-beam to create patterns aligned with the pre-existing features on the wafer. Aldehyde-terminated polyamidoamine dendrimers were assembled in a layer-by-layer fashion in the ablated patterns to allow the covalent immobilization of oligonucleotide probes. The functionality of the attached oligonucleotides was demonstrated by the hybridization of fluorescently labeled complementary target oligonucleotides. The hybridized target oligonucleotides could be stripped and the regenerated surface bound probe oligonucleotides could be rehybridized with complementary target oligonucleotide.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Cornell University, 14853, Ithaca, NY, USA

    Parijat Bhatnagar

  2. Department of Microbiology, Cornell University, 14853, Ithaca, NY, USA

    Sonny S Mark

  3. Department of Food Science, Cornell University, 14853, Ithaca, NY, USA

    Il Kim & Hongyu Chen

  4. Department of Electrical and Computer Engineering, Cornell University, 14853, Ithaca, NY, USA

    Brad Schmidt & Michal Lipson

  5. Department of Biomedical Engineering, Microbiology, and Food Science, Cornell University, 14853, Ithaca, NY, USA

    Carl A Batt

Authors
  1. Parijat Bhatnagar
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  2. Sonny S Mark
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  3. Il Kim
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  4. Hongyu Chen
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  5. Brad Schmidt
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  6. Michal Lipson
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  7. Carl A Batt
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Bhatnagar, P., Mark, S.S., Kim, I. et al. Electron-Beam Directed Layer-by-Layer Assembly of Dendrimer Scaffold for Biomolecule Patterning. MRS Online Proceedings Library 921, 408 (2006). https://doi.org/10.1557/PROC-0921-T04-08

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  • DOI: https://doi.org/10.1557/PROC-0921-T04-08

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