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Design and synthesis of an antigenic mimic of the Ebola glycoprotein

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Abstract

An antigenic mimic of the Ebola glycoprotein was synthesized and tested for its ability to be recognized by an anti-Ebola glycoprotein antibody. Epitope-mapping procedures yielded a suitable epitope that, when presented on the surface of a nanoparticle, forms a structure that is recognized by an antibody specific for the native protein. This mimic-antibody interaction has been quantitated through ELISA and QCM-based methods and yielded an affinity (Kd = 12 × 10−6 M) within two orders of magnitude of the reported affinity of the native Ebola glycoprotein for the same antibody. These results suggest that the rational design approach described herein is a suitable method for the further development of protein-based antigenic mimics with potential applications in vaccine development and sensor technology.

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Acknowledgments

This work is funded in part by the National Institutes of Health (NIH; Grant RO1 GM 076479) and the NIH Southeast Regional Center of Excellence for Biodefense (Grant 5 U54 AI57157). We also acknowledge Larry Liao and Bart Haynes for providing the monoclonal antibody 15H10.

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Authors and Affiliations

  1. Department of Chemistry, Vanderbilt University, Nashville, Tennessee, 37235, USA

    Ryan D. Rutledge, Brian J. Huffman, David E. Cliffel & David W. Wright

Authors
  1. Ryan D. Rutledge
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  2. Brian J. Huffman
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  3. David E. Cliffel
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  4. David W. Wright
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Corresponding author

Correspondence to David W. Wright.

Appendix: Design and Synthesis of an Immunological Mimic of the Ebola Glycoprotein

Appendix: Design and Synthesis of an Immunological Mimic of the Ebola Glycoprotein

FIG. A1
figure FA1

TEM image of Au-tiopronin nanoparticles.

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FIG. A2
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Histogram of Au-tiopronin particles.

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FIG. A3
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NMR of Au-tiopronin particles.

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FIG. A4
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Thermogravimetric analysis of Au-tiopronin particles.

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FIG. A5
figure FA5

NMR of AuTioproninEbola-3 nanoparticles after Ebola-3 place exchange.

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Rutledge, R.D., Huffman, B.J., Cliffel, D.E. et al. Design and synthesis of an antigenic mimic of the Ebola glycoprotein. Journal of Materials Research 23, 3161–3168 (2008). https://doi.org/10.1557/JMR.2008.0384

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  • DOI: https://doi.org/10.1557/JMR.2008.0384

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