Abstract
Bacillus thuringiensis ssp. israelensis (Bti) is increasingly used as an ecologically friendly anti-mosquito agent. The bacterium cells undergo fermentation in dilute suspensions; before practical use, therefore it is necessary to concentrate the suspensions. Aggregation by polymers is a powerful tool with which to regulate the stability of suspensions. Typically, polymers at low concentrations destabilize and at high concentrations stabilize colloidal systems. Bti suspensions can be flocculated efficiently by either cationic or anionic polyelectrolytes. Cationic polyelectolytes were found to be the most efficient flocculants for bacterial suspensions. It was shown that the degree of toxicity of the flocculated Bti suspensions for biting mosquito larvae was in the same range than in non-flocculated suspension.
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References
Baran, A. A. (1990) Flocculants in biotechnology. Khimiya Publishing House, Leningrad, pp. 1–156. (In Russian.)
Baran, A. A. (1988) Flocculation of cellular suspension by polyelectrolytes. Colloids and Surfaces 31, 259–264.
Baran, A. A. (1986) Polymers in disperse systems. Naukova Dumka Publishing House, Kiev, pp. 1–206. (In Russian.)
Bárány, S., Gregory, J. (1996) Flocculation of kaolin suspensions by cationic polyelectrolytes. Colloid J. 58, 9–14.
Bárány, S., Teszlenko, A. (1992) Flocculation of microorganism suspensions. Magyar Kémiai Folyóirat 12, 463–476. (In Hungarian.)
Bárány, S. (2000) Polymers in disperse systems. In: Csákvári, B. (ed.) Progress in Chemistry. Vol. 88. Akadémiai Kiadó, Budapest, pp. 121–152. (In Hungarian.)
Bhattacharya, P. R. (1998) Microbial control of mosquitoes with special emphasis on bacterial control. Indian J. Malariol. 35, 206–224.
Federici, B. A. (1995) The future of microbial insecticides as vector control agents. J. Am. Mosq. Control. 11, 260–268.
Gill, S. S., Cowles, E. A., Pietrantonio, P. V. (1992) The mode of action of Bacillus thuringiensis endotoxins. Annu. Rev. Entomol. 37, 615–636.
Gregory, J. (1985) Turbidity flocculation in flowing suspensions. J. Colloid Interf. Sci. 105, 357–371.
Gregory, J. (1988) Polymer adsorption and flocculation in sheared suspensions. Colloids and Surfaces 31, 231–253.
Guan, X., Chen, J., Huang, Z., Tang, Y., Gao, R. (1998) Batch fermentation and optimization of media for Bacillus thuringiensis. Chin. J. Biotechnol. 14, 45–51.
Arnold, E. G., Lenore, S. C., Andrew, D. E. (1992) Standard Methods. American Public Health Association, Washington.
Klowden, M. J., Bulla, L. A. Jr. (1984) Oral toxicity of Bacillus thuringiensis subsp. israelensis to adult mosquitoes. Appl. Environ. Microb. 48, 665–667.
Kuppusamy, M., Balaraman, K. (1991) Fed-batch fermentation studies with Bacillus thuringiensis H-14 synthesising endotoxin. Indian J. Exp. Biol. 29, 1031–1034.
Margalit, J., Dean, D. (1985) The story of Bacillus thuringiensis var. israelensis (B.t.i.). J. Am. Mosq. Control. 1, 1–7.
Mihályi, F., Gulyás, M. (1963) The biting mosquitoes of Hungary. Akadémiai Kiadó, Budapest. (In Hungarian.)
Rasnitsyn, S. P., Voitsik, A. A., Iasiukevich, V. V. (1993) The effect of water temperature on the action of bacterial insecticides against mosquito larvae. Med. Parazitol. (Moscow) 1, 8–10. (In Russian.)
Smith, R. A. (1982) Effect of strain and medium variation on mosquito toxin production by Bacillus thuringiensis var. israelensis. Can. J. Microbiol. 28, 1089–1092.
Sneath, H. A. P. (1986) Systematic bacteriology. In: Holt, J. G. (ed.) Endospore-forming Gram-Positive Rods and Cocci. Williams & Wilkins Press, Baltimore, pp. 1104–1111.
Water Quality. Determination of the Daphnia magna Strauss mobility inhibition (1998) Acute toxicity test. (Hungarian Standard. MSZ EN ISO 6341.) pp. 1–13.
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Szepesszentgyörgyi, Á., Bárány, S. & Mécs, I. Flocculation of Bacillus thuringiensis Var. israelensis Suspension and Its Efficacy Against Mosquito Larvae. BIOLOGIA FUTURA 56, 151–164 (2005). https://doi.org/10.1556/ABiol.56.2005.1-2.15
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DOI: https://doi.org/10.1556/ABiol.56.2005.1-2.15