Abstract
A pot experiment was performed as factorial based on randomized complete block design with three replications, to assess the effects of 1 mM spermidine (SPD) and 1 mM putrescine (PUT) on Indian mustard (Brassica Juncea L.) under different levels of watering (100, 75, 50 and 25% of field capacity). Chlorophyll a and b contents decreased, but the ratio of Chl a/b and carotenoid content increased with decreasing water supply. Foliar sprays of polyamines improved chlorophylls a and b and carotenoid contents, while the ratio of Chl a/b was reduced by these growth regulators. Relative water content, glycine betaine, proteins and soluble sugars contents were increased, but proline content was decreased by exogenous polyamines under limited water supply. Antioxidant enzyme (POX, CAT, SOD and APX) activities were enhanced by drought stress and polyamine treatments. This resulted in lower electrolyte leakage and lipid peroxidation (less MDA) under stressful conditions. The present results indicate that exogenous polyamines such as putrescine and spermidine can alleviate some of the deleterious impacts of water limitation on Indian mustard.
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References
Anderson, J. W., Rowan, K. S. (1966) The effect of 6-furfurylaminopurine on senescence in tobaccoleaf tissue after harvest. Biochem. J. 98, 401–404.
Arnon, D. I. (1949) Copper enzymes in isolated chloroplasts polyphenol oxidase in Beta vulgaris. Plant Physiol. 24, 1–14.
Bartels, D., Sunkar, R. (2005) Drought and salt tolerance in plants. Crit. Rev. Sci. 24, 23–58.
Bates, L. S., Waldren, R. P., Teare, I. D. (1973) Rapid determination of free proline for water-stress studies. Plant. Soil. 39, 205–207.
Biswal, U. C., Mohanty, P. (1976) Ageing induced changes in photosynthetic electron transport of detached barley leaves. Plant Cell Physiol. 17, 323–331.
Blum, A., Ebercon, A. (1981) Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Sci. 21, 43–47.
Boyer, J. S. (1992) Plant productivity and environment. Science 218, 443–448.
Bradford, M. M. (1976) A rapid sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
Caldarera, C. M., Rossoni, C., Casti, A. (1976) Involvement of polyamines in ribonucleic acid synthesis as a possible biological function. Ital. J. Biochem. 25, 33–55.
Chen, T. H. H., Murata, N. (2008) Glycine betaine: an effective protectant against abiotic stress in plants. Trends in Plant Sci. 13, 499–505.
Demirevska, K., Zasheva, D., Dimitrov, R., Simova-Stoilova, L., Stamenova, M., Feller, U. (2009) Drought stress effects on Rubisco in wheat: Changes in the Rubisco large subunit. Acta Physiol. Plant. 31, 1129–1138.
Dennis, D. T., Stubbs, N., Coulate, T. P. (1967) The inhibition of brussels sprout leaf senescence by kinins. Can. J. Bot. 45, 1019–1024.
Dunbar, B., Elthon, E., Osterman, C. (1997) Identification of plant mitochondrial proteins: a procedure linking two-dimensional electrophoresis to protein sequencing from PVDF membranes using a fastblot cycle. Plant Mol. Biol. Report 15, 46–61.
Foyer, C. H., Noctor, G. (2005) Redox homeostasis and antioxidant signaling: a metabolic interface between stress perception and physiological responses. Plant Cell. 17, 1866–1875.
Giannopolitis, C. N., Ries, S. K. (1977) Superoxide dismutases. I. Occurrence in higher plants. Plant Physiol. 59, 309–314.
Gueta-Dahan, Y., Yaniv, Z., Zilinskas, B. A., Ben-Hayyim, G. (1997) Salt and oxidative stress: similar and specific responses and their relation to salt tolerance in citrus. Planta 203, 460–469.
Grieve, C. M., Grattan, S. R. (1983) Rapid assay for the determination of water soluble quaternary ammonium compounds. Plant Soil. 70, 303–307.
Hakeem, K. R., Chandna, R., Ahmad, P., Ozturk, M., Iqbal, M. (2012) Relevance of proteomic investigations in plant stress physiology. OMICS J. Integr. Biol. 16, 621–635.
Janero, D. R. (1990) Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic. Biol. Med. 9, 515–540.
Keyvan, S. (2010) The effects of drought stress on yield, relative water content, proline, soluble carbohydrates and chlorophyll of bread wheat cultivars. J. Animal Plant Sci. 8, 1051–1060.
Kochert, G. (1978) Carbohydrate determination by the phenol sulfuric acid method. In: Hellebust, J. A., Craigie, J. S. (eds) Handbook of Physiological Methods–Physiological and Biochemical Methods. Cambridge University Press, London, pp. 96–97.
Kumar, A., Altabella, T., Taylor, M. A., Tiburcio, A. F. (1997) Recent advances in polyamine research. Trends Plant Sci. 2, 124–130.
Liu, Y., Fiskum, G., Schubert, D. (2002) Generation of reactive oxygen species by mitochondrial electrontransport chain. J. Neurochem. 80, 780–787.
Maliwal, G. L., Thakkar, K. R., Sonani, V. V., Patel, P. H., Trivedi, S. N. (1998) Response of mustard (Brassica juncea L.) to irrigation and fertilization. Ann. Agric. Res. 19, 353–359.
Masoumi, A., Kafi, M., Khazaei, H. R., Davari, K. (2010) Effect of drought stress on water status, electrolyte leakage and enzymatic antioxidants of Kochia (Kochia Scoparia) under saline condition. Pak. J. Bot. 42, 3517–3524.
Mittler, R. (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7, 405–410.
Mittler, R., Vanderauwera, S., Gollery, M., Van Breusegem, F. (2004) Reactive oxygen gene network of plants. Trends Plant Sci. 9, 490–498.
Mullineaux, P. M., Baker, N. R. (2010) Oxidative stress: antagonistic signaling for acclimation or cell death? Plant Physiol. 154, 521–525.
Munns, R. (2002) Comparative physiology of salt and water stress. Plant Cell Environ. 25, 239–250.
Nakano, Y., Asada, K. (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22, 867–880.
Noctor, G., Foyer, C. H. (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu. Rev. Plant Biol. 49, 249–279.
Pastori, G. M., Foyer, C. H. (2002) Common components, networks, and pathways of cross-tolerance to stress. The central role of “redox” and abscisic acid-mediated controls. Plant Physiol. 129, 460–468.
Patel, J. R. (1999) Effect of irrigation and nitrogen on mustard. J. Maharashtra Agric. Univ. 23, 259–261.
Prochaska, L. J., Gross, E. L. (1977) Evidence for the location of divalent cation binding sites on the chloroplast membrane. J. Membr. Biol. 36, 13–32.
Quan, R., Hu, S., Zhang, Z., Zhang, H., Huang, R. (2010) Over expression of an ERF transcription factor TSRF1 improves rice drought tolerance. Plant Biotechnol. J. 8, 476–488.
Rachmilevitch, S., DaCosta, M., Huang, B. (2006) Physiological and biochemical indicators for stress tolerance. In: Huang, B. (ed.). Plant-environment interactions. CRC Press, Boca Raton, Florida, pp. 321–356.
Resketo, P., Szabo, L. (1992) The effect of drought on development and yield components of soybean. Int. J. Trop. Agric. 8, 347–354.
Ritchie, S. W., Nguyan, H. T., Holaday, A. S. (1990) Leaf water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Sci. 30, 105–111.
Riznsky, L. H., Liang, H., Hittler, R. (2002) The combined effect of drought stress and heat shock on gene expression in tobacco plant. Plant Physiol. 130, 1143–1151.
Roychoudhury, A., Basu, S., Sengupta, D. N. (2011) Amelioration of salinity stress by exogenously applied spermidine or spermine in three varieties of indica rice differing in their level of salt tolerance. J. Plant Physiol. 168, 317–328.
Sharma, P., Dubey, R. S. (2005) Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regul. 46, 209–221.
Sharma, P., Jha, A. B., Dubey, R. S., Pessarakli, M. (2012) Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J. Bot. 7, 1–26.
Singh, B. K., Sharma, S. R., Singh, B. (2010) Antioxidant enzymes in cabbage: variability and inheritance of superoxide dismutase, peroxidase and catalase. Sci. Hortic. 124, 9–13.
Sonntag, N. O. V. (1995) Industrial utilization of long-chain fatty acids and their derivatives. In: Kimber, D. S., McGregor, D. I. (eds), Brassica Oilseeds. CAB International, Oxford shire, pp. 339–352.
Tabor, C. W., Tabor, H. (1999) It all started on a streetcar in Boston. Annu. Rev. Plant Biol. 68, 1–32.
Tanou, G., Ziogas, V., Belghazi, M., Christou, A., Filippou, P., Job, D. (2014) Polyamines reprogram oxidative and nitrosative status and the proteome of citrus plants exposed to salinity stress. Plant Cell Environ. 37, 864–885.
Valentovic, P., Luxova, M., Kolarovic, L., Gasparikova, O. (2006) Effect of osmotic stress on compatible solutes content, membrane stability and water relations in two maize cultivars. Plant Soil Environ. 52, 186–191.
Wang, J., Liu, J. H. (2009) Change in free polyamine contents and expression profiles of two polyamine biosynthetic genes in citrus embryo genic callus under abiotic stresses. Biotechnol. Eq. 29, 1289–1293.
Xiong, J., Zhang, L., Fu, G., Yang, Y., Zhu, C., Tao, L. (2012) Drought-induced proline accumulation is uninvolved with increased nitric oxide, which alleviates drought stress by decreasing transpiration in rice. J. Plant Res. 125, 155–164.
Yokota, A., Takahara, K., Akashi, K. (2006) Water stress. In: Madhava-Rao, K. V., Raghavendra, A. S., Janardhan-Reddy, K. (eds), Physiology and Molecular Biology of Stress Tolerance in Plants. Springer, Berlin. pp. 15–40.
Zhang, Y., Zhang, H., Zou, Z. R., Liu, Y., Hu, X. H. (2015) Deciphering the protective role of spermidine against saline-alkaline stress at physiological and proteomic levels in tomato. Phytochem. 110, 13–21.
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Mostafaei, E., Zehtab-Salmasi, S., Salehi-Lisar, Y. et al. Changes in Photosynthetic Pigments, Osmolytes and Antioxidants of Indian Mustard by Drought and Exogenous Polyamines. BIOLOGIA FUTURA 69, 313–324 (2018). https://doi.org/10.1556/018.68.2018.3.7
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DOI: https://doi.org/10.1556/018.68.2018.3.7