Abstract
Assays assessing non-enzymatic hydrogen peroxide antioxidant capacities are often hampered by the high UV absorption of the sample itself. This is a typical problem in studies using plant extracts with high polyphenol content. Our assay is based on comparing the 405 nm absorption of the product of potassium iodine and hydrogen peroxide in the presence and absence of a putative hydrogen peroxide reactive antioxidant. This method is free of interference with either hydrogen peroxide or antioxidant self-absorption and it is also suitable for high-throughput plate reader applications.
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Abbreviations
- Quercetin:
-
2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromen-4-one
- ROS:
-
reactive oxygen species
- Trolox:
-
6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid
- UV:
-
ultraviolet light.
References
Halliwell, B., Gutteridge, J. M. C. (2006) Free Radicals in Biology and Medicine. Clarendon Press, Oxford.
Neill, S. J., Desikan, R., Clarke, A., Hurst, R., Hancock, J. (2002) Hydrogen peroxide and nitric oxide as signalling molecules in plants. J. Exp. Bot. 53, 1237–1247.
Noctor, G., Foyer, C. H. (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu. Rev. Plant Phys. 49, 249–279.
Kähkönen, M. P., Hopia, I. A., Vuorela, J. H., Rauha, J.-P., Pihlaja, K., Kujala, S. T., Heinonen, M. (1999) Antioxidant activity of plant extracts containing phenolic compounds. J. Agric. Food Chem. 47, 3954–3962.
Keser, S., Celik, S., Turkoglu, S., Yilmaz, Ö., Turkoglu, I. (2012) Hydrogen peroxide radical scavenging and total antioxidant activity of hawthorn. Chem. J. 2, 9–12.
Fernando, C. D., Soysa, P. (2015) Optimized enzymatic colorimetric assay for determination of hydrogen peroxide (H2O2) scavenging activity of plant extracts. MethodsX. 2, 283–291.
Shah, V., Baldrian, P., Eichlerova, I., Dave, R., Madamwar, D., Nerud, F., Gross, R. (2006) Influence of dimethyl sulfoxide on extracellular enzyme production by Pleurotus ostreatus. Biotechnol. Lett. 28, 651–655.
Junglee, S., Urban, L., Sallanon, H., Lopez-Lauri, F. (2014) Optimized assay for hydrogen peroxide determination in plant tissue using potassium iodide. American J. Anal. Chem. 5, 730–736.
Formica, J. V., Regelson, W. (1995) Review of the biology of quercetin and related bioflavonoids. Food Chem. Toxicol. 33, 1061–1080.
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Csepregi, K., Hideg, É. A Novel Procedure to Assess the Non-Enzymatic Hydrogen-Peroxide Antioxidant Capacity of Metabolites with High UV Absorption. BIOLOGIA FUTURA 67, 447–450 (2016). https://doi.org/10.1556/018.67.2016.4.11
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DOI: https://doi.org/10.1556/018.67.2016.4.11