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Cereal Research Communications

, Volume 43, Issue 1, pp 133–143 | Cite as

Celiac Disease-Specific Prolamin Peptide Content of Wheat Relatives and Wild Species Determined by ELISA Assays and Bioinformatics Analyses

  • Gy. Gell
  • K. Kovács
  • I. Molnár
  • Zs. Bugyi
  • S. Tömösközi
  • A. JuhászEmail author
Quality and Utilization
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Abstract

Enzyme-linked immunosorbent assays (ELISAs) are widely used to determine gluten contamination in gluten-free and low gluten food samples. ELISA assays developed using monoclonal antibodies against known toxic peptides have an advantage in the identification of toxic prolamin content in protein extracts of different food samples, as well as raw materials. R5 and G12 monoclonal antibodies specific for two known toxic peptides used in commercially available gluten ELISA assays were applied to test toxic peptide contents in wheat relatives and wild wheat species with different genome composition and complexity. Although the R5 peptide content showed some correlation with ploidy levels in Triticum species, there was a high variance among Aegilops species. Some of the analysed diploid Aegilops species showed extremely high R5 peptide contents. Based on the bioinformatics analyses, the R5 peptide was present in most of the sulphur rich prolamins in all the analysed species, whereas the G12 epitope was exclusively present in alpha gliadins. High variation was detected in the position and frequency of epitopes in sequences originating from the same species, thus highlighting the importance of genotypic variation within species. Identification of new prolamin alleles of wheat relatives and wild wheat species is of great importance in order to find germplasm for special end-use quality purposes as well as development of food with reduced toxicity.

Keywords

ELISA R5 epitope G12 epitope celiac disease cereal species 

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© Akadémiai Kiadó, Budapest 2015

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Gy. Gell
    • 1
  • K. Kovács
    • 1
  • I. Molnár
    • 2
  • Zs. Bugyi
    • 3
  • S. Tömösközi
    • 3
  • A. Juhász
    • 1
    Email author
  1. 1.Applied Genomics Department, Agricultural Institute, Centre for Agricultural ResearchHASMartonvásárHungary
  2. 2.Department of Plant Genetic Resources and Organic Breeding, Agricultural Institute, Centre for Agricultural ResearchHASMartonvásárHungary
  3. 3.Department of Applied Biotechnology and Food ScienceBudapest University of Technology and EconomicsBudapestHungary

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