Celiac Disease-Specific Prolamin Peptide Content of Wheat Relatives and Wild Species Determined by ELISA Assays and Bioinformatics Analyses
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Enzyme-linked immunosorbent assays (ELISAs) are widely used to determine gluten contamination in gluten-free and low gluten food samples. ELISA assays developed using monoclonal antibodies against known toxic peptides have an advantage in the identification of toxic prolamin content in protein extracts of different food samples, as well as raw materials. R5 and G12 monoclonal antibodies specific for two known toxic peptides used in commercially available gluten ELISA assays were applied to test toxic peptide contents in wheat relatives and wild wheat species with different genome composition and complexity. Although the R5 peptide content showed some correlation with ploidy levels in Triticum species, there was a high variance among Aegilops species. Some of the analysed diploid Aegilops species showed extremely high R5 peptide contents. Based on the bioinformatics analyses, the R5 peptide was present in most of the sulphur rich prolamins in all the analysed species, whereas the G12 epitope was exclusively present in alpha gliadins. High variation was detected in the position and frequency of epitopes in sequences originating from the same species, thus highlighting the importance of genotypic variation within species. Identification of new prolamin alleles of wheat relatives and wild wheat species is of great importance in order to find germplasm for special end-use quality purposes as well as development of food with reduced toxicity.
KeywordsELISA R5 epitope G12 epitope celiac disease cereal species
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- Camarca, A., Anderson, R.P., Mamone, G., Fierro, O., Facchiano, A., Costantini, S., Zanzi, D., Sidney, J., Auricchio, S., Sette, A., Troncone, R., Gianfrani, C. 2009. Intestinal T cell responses to gluten peptides are largely heterogeneous: Implications for a peptide-based therapy in celiac disease. J. Immunol. 182:4158–4166.CrossRefGoogle Scholar
- Catassi, C., Fasano, A. 2008. Celiac disease. In: Arendt, E.K., Dal Bello, F. (eds), Gluten-Free Cereal Products and Beverages. Academic Press, San Diego, CA, USA, pp. 1–28.Google Scholar
- Codex Standard for Foods for Special Dietary Use for Persons Intolerant to Gluten — CODEX STAN 118 — 1979, adopted in 1979; amended in 1983; revised in 2008, Codex Alimentarius, International Food Standards. Rome, Italy.Google Scholar
- Morón, B., Cebolla, Á., Manyani, H., Álvarez-Maqueda, M., Megías, M., Thomas, M. del C., López, M.C., Sousa, C. 2008. Sensitive detection of cereal fractions that are toxic to celiac disease patients by using monoclonal antibodies to a main immunogenic wheat peptide. Am. J. Clin. Nutr. 87:405–414.CrossRefGoogle Scholar
- Sapone, A., Bai, J.C., Ciacci, C., Dolinsek, J., Green, P.H.R., Hadjivassiliou, M., Kaukinen, K., Rostami, K., Sanders, D.S., Schumann, M., Ullrich, R., Villalta, D., Volta, U., Catassi, C., Fasano, A. 2012. Spectrum of gluten-related disorders: Consensus on new nomenclature and classification. BMC Medicine 10:13.CrossRefGoogle Scholar
- van Herpen, T.W.J.M., Goryunova, S.V., van der Schoot, J., Mitreva, M., Salentijn, E.M.J., Vorst, O., Schenk, M.F., van Veelen, P.A., Koning, F., van Soest, L.J.M., Vosman, B., Bosch, D., Hamer, R.J., Gilissen, L.J.W.J., Smulders, M.J.M. 2006. Alpha-gliadin genes from the A, B, and D genomes of wheat contain different sets of celiac disease epitopes. BMC Genomics 7:1.CrossRefGoogle Scholar
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