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Cereal Research Communications

, Volume 40, Issue 2, pp 204–209 | Cite as

SSAP Markers Based on a Novel Ty1-Copia Like Element Are a Powerful Tool for the Assessment of Genetic Diversity in Rye (Secale Cereale L.) Inbred Lines

  • H. Bolibok-BrbągoszewskaEmail author
  • N. Zabierzewska
  • A. Hromada-Judycka
  • L. Krzewska
Article

Abstract

A partial sequence of a Ty1-copia retrotransposon was isolated from the genome of the rye (Secale cereale L.) inbred line L318 using degenerate primers targeted at the RNaseH sequence. Its putative 3’LTR region exhibited similarity to LTRs of known plant retrotransposons such as BARE-1, while no significant similarity to known rye retrotransposon sequences was found. A Sequence Specific Amplification Polymorphism (SSAP) protocol employing primer designed to correspond to the putative polypurine tract was developed and used for analysis of 30 rye inbred lines. Values of the parameters calculated to evaluate the efficiency of the method as well as the results of cluster analysis demonstrate that it is a powerful tool for genetic diversity analyses of rye.

Keywords

genetic diversity molecular markers retrotransposon rye SSAP Secale cereale Ty1-copia 

Abbreviations

ISSR

inter simple sequence repeat

LTR

long terminal repeat

MI

marker index

PIC

polymorphic information content

SAMPL

selective amplification of polymorphic microsatellite loci

SSAP

sequence specific amplification polymorphism

SSR

simple sequence repeat

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Copyright information

© Akadémiai Kiadó, Budapest 2012

Authors and Affiliations

  • H. Bolibok-Brbągoszewska
    • 1
    Email author
  • N. Zabierzewska
    • 1
  • A. Hromada-Judycka
    • 1
  • L. Krzewska
    • 1
  1. 1.Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture and Landscape ArchitectureWarsaw University of Life Sciences (SGGW)WarsawPoland

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