Validation of Molecular Markers for Pre-Harvest Sprouting Resistance in Bread Wheat

Abstract

Pre-harvest sprouting (PHS) in bread wheat (Triticum aestivum L.) is one of the major abiotic constraints influencing production of high quality grain. Selection for pre-harvest sprouting (PHS) resistance in bread wheat (Triticum aestivum L.) in early generations is difficult because it is expressed as a quantitatively inherited trait and subject to environmental effects. The objectives of this study were to validate a major quantitative trait locus (QTL) for PHS resistance on chromosome 4A in bread wheat and to isolate near-isogenic lines for this QTL using marker-assisted selection. A total of 60 Canadian wheat cultivars and experimental lines were screened with three SSR markers in a QTL region for PHS resistance. The SSR markers DuPw004, barc170 and wmc650 explained 67%, 75% and 60% of total variation in germination (%), respectively, among different wheat genotypes. Marker assisted back crossing with DuPw004 reduced the population size in BC1 F1 and BC2 F1 generation by 41% and 59%, respectively. A survey of pedigrees of different genotypes revealed that the parental line RL4137 is a major source of increased PHS resistance in a number of western Canadian wheat cultivars. Microsatellite markers (DuPw004, barc170 and wmc650) will be useful for plant breeders to pyramid QTL from different PHS resistance sources.

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Correspondence to R. N. Chibbar.

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Communicated by L. Bona

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Singh, R., Hucl, P., Båga, M. et al. Validation of Molecular Markers for Pre-Harvest Sprouting Resistance in Bread Wheat. CEREAL RESEARCH COMMUNICATIONS 40, 194–203 (2012). https://doi.org/10.1556/CRC.40.2012.2.3

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Keywords

  • pre-harvest sprouting
  • molecular marker
  • marker validation
  • marker assisted selection
  • wheat (Triticum aestivum L.)