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Cereal Research Communications

, Volume 40, Issue 4, pp 542–551 | Cite as

Molecular Characterization of LMW-GS Genes From C, N, U and Ss Genomes among Aegilops Species

  • S. L. Wang
  • D. Chen
  • G. F. Guo
  • T. Zhang
  • S. S. Jiang
  • X. X. Shen
  • D. Perovic
  • S. ProdanovicEmail author
  • Y. M. YanEmail author
Quality and Utilization

Abstract

In this work, 9 novel LMW-GS genes (6 LMW-m and 3 LMW-i type) from 4 diploid and 1 tetraploid Aegilops species were amplified and cloned by allelic-specific PCR. Analysis of the deduced amino acid sequences showed that 7 and 2 LMW-GS had 9 and 7 cysteines, respectively. Four LMW-m type subunits genes had an extra cysteine at the C-terminal III, which could form intermolecular disulphide bonds to extend the chains, and therefore would facilitate to form larger gluten polymers. This suggested that these genes are expected to be used as candidate genes for wheat quality improvement. The correlation between specific N-terminal sequences and a decapeptide deletion in the C-terminal II in LMW-GS encoded by D genome was found. Particularly, if LMW-GS possessed a METRCIPG-N-terminal beginning sequences and a decapeptide (LGQCSFQQPQ) deletion in the C-terminal II, they could be encoded by D genome.

Keywords

Aegilops species LMW-GS genes N-terminal domain conserved cysteines 

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© Akadémiai Kiadó, Budapest 2012

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • S. L. Wang
    • 1
  • D. Chen
    • 1
  • G. F. Guo
    • 1
  • T. Zhang
    • 1
  • S. S. Jiang
    • 1
  • X. X. Shen
    • 1
  • D. Perovic
    • 2
  • S. Prodanovic
    • 3
    Email author
  • Y. M. Yan
    • 1
    Email author
  1. 1.Key Laboratory of Genetics and Biotechnology, College of Life ScienceCapital Normal UniversityBeijingChina
  2. 2.Julius Kuehn-Institute (JKI)QuedlinburgGermany
  3. 3.Faculty of AgricultureUniversity of BelgradeBelgradeSerbia

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