A retrospective analysis of HMW and LMW glutenin alleles of cultivars bred in Martonvásár, Hungary

Abstract

The glutenin allele gene-pool, the distribution of the individual alleles on the 6 loci coding for glutenin subunits and their combinations were determined in a sample population containing 107 cultivars bred and grown in Martonvásár, Hungary at the Agricultural Research Institute of the Hungarian Academy of Sciences. The database is based on the results of three independent analytical procedures carried out using the traditional SDS-PAGE based allele identification, the state-of-art MALDI-TOF technology and the high throughput capillary electrophoresis based on the lab-on-a-chip technique. The usefulness of integrating the information on both HMW GS and LMW GS allelic composition for future genetic and technological improvement is discussed.

Abbreviations

HMWGS:

high molecular weight glutenin subunits

LMWGS:

low molecular weight glutenin subunits

SDS-PAGE:

sodium dodecyl sulphate polyacrylamide gel electrophoresis

MALDI-TOF-MS:

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

LOC-CE:

lab-on-a-chip capillary electrophoresis

References

  1. Appels, R., Gustafson, J.P., O’Brien, L. 2001. Wheat breeding in the new century: Applying molecular genetic analysis of key quality and agronomic traits. Aust. J. Agric. Res. 52:1043–1417.

    Article  Google Scholar 

  2. Balázs, G., Baracskai, I., Nádosi, M., Harasztos, A., Békés, F., Tömösközi, S. 2011. Lab-on-a-chip technology in cereal science: Analytical properties and possible application areas. Acta Alimentaria (in press).

  3. Bedõ, Z., Vida, Gy., Láng, L., Karsai, I. 1998. Breeding for breadmaking quality using old Hungarian wheat varieties. Euphytica 100:179–182.

    Article  Google Scholar 

  4. Bedõ, Z., Vida, Gy., Láng, L., Juhász, A., Karsai, I. 1999. Breeding a wheat variety with different lines for technological quality and HMW glutenin composition. J. Genet. Breed. 53:57–62.

    Google Scholar 

  5. Békés, F., Kemény, S., Morell, M. 2006a. An integrated approach to predicting end-product quality of wheat. Eur. J. Agron. 25:155–162.

    Article  Google Scholar 

  6. Békés, F., Cavanagh, C.R., Martinov, S., Bushuk, W., Wrigley, C.W. 2006b. The Gluten Composition of Wheat Varieties and Genotypes. Part III. Composition table for HMW-GS. http://www.aaccnet.org/grainbin/pdfs/II_HMW_Subunits.pdf

  7. Békés, F., Cavanagh, C.R., Martinov, S., Bushuk, W., Wrigley, C.W. 2006c. The Gluten Composition of Wheat Varieties and Genotypes. Part II. Composition table for LMW-GS. http://www.aaccnet.org/grainbin/pdfs/III_LMWSubunits.pdf

  8. Branlard, G., Dardevet, M., Amiour, M., Igrejas, G. 2003. Allelic diversity of HMW and LMW glutenin subunits and omegagliadins in French bread wheat (Triticum aestivum L.). Genet. Resour. Crop Evol. 50:669–679.

    CAS  Article  Google Scholar 

  9. Cornish, G.B., Békés, F., Eagles, H.A., Payne, P.I. 2006. Prediction of dough properties for bread wheats (Chapter 8). In: Wrigley, C.W., Békés, F., Bushuk, W. (eds), Gliadin and Glutenin. The Unique Balance of Wheat Quality. AACCI Press, St Paul, MN, USA, pp. 243–280.

    Google Scholar 

  10. Eagles, H.A., Hollamby, G.J., Gororo, N.N., Eastwood, R.F. 2002. Estimation and utilisation of glutenin gene effects from the analysis of unbalanced data from wheat breeding programs. Aust. J. Agric. Res. 53:1047–1057.

    CAS  Article  Google Scholar 

  11. Gupta, R.B., Shepherd, K.W. 1990. Two-step one-dimensional SDS-PAGE analysis for LMW-GS. 2. Genetic control of the subunits in species related to wheat. Theor. Appl. Genet. 80:65–74.

    CAS  Article  Google Scholar 

  12. Gupta, R.B., Békés, F., Wrigley, C.W. 1991. Prediction of physical dough properties from glutenin subunit composition in bread wheats: correlation studies. Cereal Chem. 68:328.

    CAS  Google Scholar 

  13. Jackson, E.A., Morel, M.H., Sontag-Sthorm, T., Branlard, G., Metaskovsky, E.V., Redaelli, R. 1996. Characterisation of HMW gliadin and LMW glutenin subunits of wheat endosperm by 2D electrphoresis and the chromosomal localisation of their controlling genes. Theor. Appl. Genet. 66:29–37.

    CAS  Article  Google Scholar 

  14. Juhász, A., Larroque, O., Tamás, L., Hsam, S.K.L., Zeller, F., Békés, F., Bedõ, Z. 2003. Bánkúti 1201 - an old Hungarian wheat variety with special storage protein composition. Theor. Appl. Genet. 107:697–704.

    Article  Google Scholar 

  15. Kammholz, S.J., Campbell, A.W., Sutherland, M.W., Hollamby, G.J., Martin, P.J., Eastwood, R.F., Barclay, I., Wilson, R.E., Brennan, P.S., Sheppard, J.A. 2001. Establishment and characterisation of wheat genetic mapping populations. Aust. J. Agric. Res. 52:1079–1088.

    CAS  Article  Google Scholar 

  16. Kussmann, M.E., Nordhoff, H., Rahbek-Nielsen, S., Haebel, M., Rossel-Larsen, L., Jakobsen, J., Gobom, E., Mirgorodskaya, A., Kroll-Kristensen, L., Roepstorff, P. 1997. MALDI-MS sample preparation techniques designed for various peptide and protein analytes. J. Mass Spectrom. 32:593–601.

    CAS  Article  Google Scholar 

  17. Liu, L., Wang, A., Appels, R., Ma, J., Xia, X., Lan, P., He, Z., Bekes, F., Yan, Y., Ma, W. 2009. A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding. J. Cereal Sci. 50:295–301.

    CAS  Article  Google Scholar 

  18. Ma, M., Wang, A., Liu, L., Békés, F., Newberry, M., Gao, L., Ma, J., Islam, S., Yan, Z., He, Y., Xia, X., Appels, R. 2011. High resolution identification of high and low molecular weight glutenin alleles by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in common wheat (Triticum aestivum L.). In: Branlard, G. (ed.), Proc. 10th International Gluten Workshop, Clermont-Ferrand, France, September 7–9, 2009, pp. 147–151.

  19. Mann, G., Diffey, S., Rampling, L., Nath, A., Kutty, I., Leyne, E., Azanza, F., Cullis, B., Smith, A., Morell, M. 2007. A QTL approach to identifying genes controlling protein, processing and baking quality attributes in wheat. In: Lookhart, G.L., Ng, P.K.W. (eds), Gluten Proteins 2006. AACCI Press, St Paul, USA, pp. 73–77.

    Google Scholar 

  20. Marchylo, B.A., Kruger, J.E., Hatcher, D.W. 1989. Quantitative reverse-phase high performance liquid chromatographic analysis of wheat storage proteins as a potential quality prediction tool. J. Cereal Sci. 9:113–130.

    CAS  Article  Google Scholar 

  21. Melas, V., Molel, M.H., Autran, J.C., Feillet, P. 1994. Simple and rapid method for purifying low molecular weight subunits of glutenin from wheat. Cereal Chem. 71:234–237.

    CAS  Google Scholar 

  22. Millar, S.J., Snape, J., Ward, J., Shewry, P.R., Belton, P., Boniface, K., Summers, R. 2008. Investigating wheat functionality through breeding and end use (FQS 23) HGCA, Project Report No. 429. Campden and Chorleywood Food Research Association, Chipping Campden, U.K.

    Google Scholar 

  23. Ng, P.K.W., Pogna, N.E., Mellini, F., Bushuk, W. 1989. Glu-1 allele compositions of the wheat cultivars registered in Canada. J. Genet. Breed. 43:53–59.

    Google Scholar 

  24. Payne, P.I., Lawrence, G.J. 1983. Catalogue of alleles for the complex loci Glu-A1, Glu-B1 and Glu-D1 which coded for HMW-GS in hexaploid wheat. Cereal Res. Comm. 11:29–35.

    Google Scholar 

  25. Payne, P.I., Nightingale, M.A., Krattiger, A.F., Holt, L.M. 1987. The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varietes. J. Sci. Food Agric. 40:51–56.

    CAS  Article  Google Scholar 

  26. Pogna, N.E., Mellini, F., Beretta, A., Deruffo, A. 1989. The high-molecular-weight glutenin subunits of common wheat cultivars grown in Italy. J. Genet. Breed. 43:17–24.

    Google Scholar 

  27. Rakszegi, M., Kárpáti, M., Lásztity, R., Bedõ, Z. 1999. Study of the LMW glutenin subunits of some old Hungarian wheat cultivars. Cereal Res. Commun. 27:293–299.

    CAS  Google Scholar 

  28. Rakszegi, M., Scholz, É., Kárpáti, M., Ganzler, K., Lásztity, R., Bedõ, Z. 2000. Study of the LMW glutenin composition of some old Hungarian wheat cultivars using capillary electrophoresis. Cereal Res. Commun. 28:417–424.

    CAS  Google Scholar 

  29. Singh, N.K., Shepherd, K.W., Cornish, G.B. 1991. Asimplified SDS-PAGE procedure for separating LMW subunits of glutenin. J. Cereal Sci. 14:203–208.

    Article  Google Scholar 

  30. Uthayakumaran, S., Listiohadi, Y., Baratta, M., Batey, I.L., Wrigley, C.W. 2006. Rapid identification and quantitation of high-molecular-weight glutenin subunits. J. Cereal Sci. 47:1–6.

    Google Scholar 

  31. Vawser, M.J., Cornish, G.B. 2004. Over-expression of HMWglutenin subunit Glu-B1 7* in hexaploid wheat varieties (Triticum aestivum). Aust. J. Agric. Res. 55:577–588.

    CAS  Article  Google Scholar 

  32. Wrigley, C.W., Békés, F., Bushuk, W. 2006. Gluten: A balance of gliadin and glutenin. In: Wrigley, C.W., Békés, F., Bushuk, W. (eds), Gliadin and Glutenin. The Unique Balance of Wheat Quality. AACCI Press, St Paul, MN, USA, pp. 3–33.

    Google Scholar 

  33. Zhen, Z., Mares, D. 1992. Asimple extraction and one-step SDS-PAGE for separating HMW and LMW glutenin subunits of wheat and high molecular weight proteins of rye. J. Cereal Sci. 15:63–78.

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to F. Békés.

Additional information

Communicated by H. Grausgruber

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Cite this article

Baracskai, I., Balázs, G., Liu, L. et al. A retrospective analysis of HMW and LMW glutenin alleles of cultivars bred in Martonvásár, Hungary. CEREAL RESEARCH COMMUNICATIONS 39, 225–236 (2011). https://doi.org/10.1556/CRC.39.2011.2.6

Download citation

Keywords

  • Gluloci
  • Glu-3
  • glutenin
  • MALDI-TOF