Characterization of wheat yellow rust resistance gene Yr17 using EST-SSR and rice syntenic region
Wheat yellow rust resistance gene Yr17 was originated from the wheat-Aegilops ventricosa introgression, and still effective on the adult plant in Southern China. The previous studies located the gene Yr17 on the translocation of 2NS-2AS using the molecular and cytological markers. In the present study, we screened new PCR-based markers to map the gene Yr17 region from the investigation of a segregating 120 F2 population. All markers including four EST-PCR markers, a SCAR (sequence characterized amplified region) and a PLUG (PCR based landmark unique gene) marker specific to Yr17 gene were mapped on the chromosome 2AS, and located on the chromosomal deletion bin 2AS5-0.8–1.00 region. Based on the wheat-rice collinearity, we found that the sequences of the Yr17 gene linked markers were comparatively matched at rice chromosome 4 and chromosome 7. However, the identified closely linked genomic sequence of Yr17 gene is most likely collinear with genomic region of rice chromosome 4. The newly produced PCR based markers closely linked to Yr17 gene will be useful for the marker-assisted selection in wheat breeding for rust resistance.
KeywordsEST SCAR markers wheat yellow rust rice synteny
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- Conley, E.J., Nduati, V., Gonzalez-Hernandez, J.L., Mesfin, A., Trudeau-Spanjers, M., Chao, S., Lazo, G.R., Hummel, D.D., Anderson, O.D., Qi, L.L., Gill, B.S., Echalier, B., Linkiewicz, A.M., Dubcovsky, J., Akhunov, E.D., Dvorák, J., Peng, J.H., Lapitan, N.L., Pathan, M.S., Nguyen, H.T., Ma, X.F., Miftahudin, G.J.P., Greene, R.A., Sorrells, M.E., Hossain, K.G., Kalavacharla, V., Kianian, S.F., Sidhu, D., Dilbirligi, M., Gill, K.S., Choi, D.W., Fenton, R.D., Close, T.J., McGuire, P.E., Qualset, C.O., Anderson, J.A. 2004. A 2600-locus chromosome bin map of wheat homoeologous group 2 reveals interstitial gene-rich islands and colinearity with rice. Genetics 168:625–637.CrossRefGoogle Scholar
- Fedak, G. 1999. Molecular aids for integration of alien chromatin through wide crosses. Genome 42:584–591.Google Scholar
- Francki, M., Carter, M., Ryan, K., Hunter, A., Bellgard, M., Appels, R. 2004. Comparative organization of wheat homoeologous group 3S and 7L using wheat-rice synteny and identification of potential markers for genes controlling xanthophyll content in wheat. Funct. Integr. Genomics 4:118–130.CrossRefGoogle Scholar
- Li, G.R., Yang, Z.J., Liu, M.J. 2000. Utilization of CIMMYT germplasm to Sichuan wheat breeding for quality. J. Sichuan Agricultural University 18:210–214.Google Scholar
- Maia, N. 1967. Obtention des bles tendres resistants au pietin-verse par croisements interspecifiques bles × Aegilops. C. R. Acad. Agric. Fr. 53:149–154 (in French).Google Scholar
- Ramakrishna, W., Dubcovsky, J., Park, Y.J., Busso, C., Emberton, J., SanMiguel, P., Bennetzen, J.L. 2002. Different types and rates of genome evolution de tected by comparative sequence analysis of orthologous segments from four cereal genomes. Genetics 162:1389–1400.PubMedPubMedCentralGoogle Scholar
- Xue, S., Zhang, Z., Lin, F., Kong, Z., Cao, Y., Li, C., Yi, H., Mei, M., Zhu, H., Wu, J., Xu, H., Zhao, D., Tian, D., Zhang, C., Ma, Z. 2008. A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags. Theor. Appl. Genet. 117:181–189.CrossRefGoogle Scholar