Identification of Sr31 and Sr36 stem rust resistance genes in wheat cultivars registered in Hungary

Abstract

In Hungary, stem rust epidemics caused by Puccinia graminis f. sp. tritici are rare, but due to the severity of infection the stem rust fungus can pose a great hazard to wheat production. As new virulent races can appear, it is important for breeders to know of the genetic background of the stem rust resistance in their cultivars. In this study, 220 winter wheat cultivars registered in Hungary in the past 35 years were investigated using molecular markers to determine the presence or absence and frequency of the two important stem rust resistance genes Sr31 and Sr36. The results indicated that both Sr31 and Sr36 genes are widespread in wheat cultivars registered in Hungary. Sr31 was detected in 24.1% of these wheats, and Sr36 in 15.9%. These genes occurred to a somewhat larger extent in the 156 local cultivars: one-third (32.1%) had the Sr31 and 18.0% the Sr36 gene. Of these, 2 cultivars (1.3%) had both genes (Sr31+ Sr36). Among the 64 foreign cultivars only 3 (4.7%) carried the Sr31 gene. In the foreign group, Sr36 was only detected in the seven Croatian cultivars. Tests also revealed possible false pedigrees for some cultivars. Inoculation tests showed that both genes were still effective. One-sixth (16.7%) of stem rust resistant cultivars did not carry the target genes indicating the possible presence of other efficient Sr genes. Data may help breeders to incorporate effective Sr genes into new cultivars.

References

  1. Bariana, H.S., Hayden, M.J., Ahmed, N.U., Bell, J.A., Sharp, P.J., McIntosh, R.A. 2001. Mapping of durable adult plant and seedling resistances to stripe rust and stem rust diseases in wheat. Aust. J. Agric. Res. 52:1247–1255.

    CAS  Article  Google Scholar 

  2. Csősz, M. 2007. Studies on Pathology and Resistance of Wheat to Rust, Powdery Mildew and Leaf Spots. Dissertation. Pannon Univ. Keszthely, Hungary. Available at: http://twilight.vein.hu/phd_dolgozatok/csoszlaszlone/Csosz_Laszlone_PhD_dolgozat_2007.pdf

    Google Scholar 

  3. Csősz, M., Bartos, P., Mesterházy, Á. 2001. Identification of stem rust resistance gene Sr36 in the wheat cultivar GK Kincso and in its derivatives. Cereal Res. Commun. 29:267–273.

    Google Scholar 

  4. Francis, H.A., Leitch, A.R., Koebner, R.M.D. 1995. Conversion of a RAPD-generated PCR product containing a novel dispersed repetitive element, into a fast and robust assay for the presence of rye chromatin in wheat. Theor. Appl. Genet. 90:636–642.

    Article  Google Scholar 

  5. Graybosch, R.A. 2001. Uneasy unions: Quality effects of rye chromatin transfers to wheat. J. Cereal Sci. 33:3–16.

    CAS  Article  Google Scholar 

  6. Jin, Y., Singh, R.P. 2006. Resistance in US wheat to recent Eastern African isolates of Puccinia graminis f. sp tritici with virulence to resistance gene Sr31. Plant Disease 90:476–480.

    CAS  Article  Google Scholar 

  7. Jin, Y., Pretorius, Z.A., Singh, R.P. 2007. New virulence within race TTKS (Ug99) of the stem rust pathogen and effective resistance genes. Phytopathol. 97:S137.

    Google Scholar 

  8. Kőszegi, B., Linc, G., Juhász, L., Láng, L., Molnár-Láng, M. 2000. Occurrence of the 1RS/1BL wheat-rye translocation in Hungarian wheat varieties. Acta Agron. Hung. 48:227–236.

    Article  Google Scholar 

  9. Láng, L., Kuti, C., Bedő, Z. 2001. Computerized data management system for cereal breeding. Wheat Global Environ. 9:561–568.

    Article  Google Scholar 

  10. Lukaszewski, A.J. 1990. Frequency of 1RS.1AL and 1RS.1BL translocations in United-States wheats. Crop Sci. 30:1151–1153.

    Article  Google Scholar 

  11. Manninger, K., Csősz, M., Falusi, J., Mesterházy, Á. 1998: Postulation of resistance genes to wheat stem rust in winter wheat genotypes from Szeged. Acta Phytopath. Entomol. Hung. 33:37–42.

    Google Scholar 

  12. McIntosh, R.A., Gyárfás, J. 1971. Triticum timopheevii as a source of resistance to wheat stem rust. Theor. Appl. Genet. 66:240–248.

    Google Scholar 

  13. Pretorius, Z.A., Singh, R.P., Wagoire, W.W., Payne, T.S. 2000. Detection of virulence to wheat stem rust gene Sr31 in Puccinia graminis f. sp. tritici in Uganda. Phytopathol. 84:203.

    CAS  Google Scholar 

  14. Purnhauser, L., Tar, M., Bóna, L., Láng, L. 2008. The occurrence of Sr31 and Sr36 stem rust resistance genes in wheat cultivars registered in Hungary in the past 25 years. In: Appels, R., Eastwood, R., Lagudah, E., Langridge, P., Mackay, M., McIntyre, L. (eds), The 11th Int. Wheat Genet. Symp. Proceedings, Sydney University Press. Available at: http://ses.library.usyd.edu.au/bitstream/2123/3415/1/P152.pdf

  15. Rabinovich, S.V. 1998. Importance of wheat-rye translocations for breeding modern cultivars of Triticum aestivum L. Euphytica 100:323–340.

    Article  Google Scholar 

  16. Rogers, S.O., Bendich, A.J. 1985. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant-tissues. Plant Mol. Biol. 5:69–76.

    CAS  Article  Google Scholar 

  17. Tsilo, T.J., Jin, Y., Anderson, J.A. 2008. Diagnostic microsatellite markers for the detection of stem rust resistance gene Sr36 in diverse genetic backgrounds of wheat. Crop Sci. 48:253–261.

    CAS  Article  Google Scholar 

  18. Vida G., Szunics, L., Szunics, L., Gál, M., Veisz, O., Láng, L., Bedő, Z. 2000. Stem rust resistance of wheat genotypes int he adult plant stage. Acta Phytopath. Entomol. Hung. 35:169–176.

    CAS  Google Scholar 

  19. Weng, Y., Azhaguvel, P., Devkota, R.N., Rudd, J.C. 2007. PCR-based markers for detection of different sources of 1AL.1RS and 1BL.1RS wheat-rye translocations in wheat background. Plant Breed. 126:482–486.

    CAS  Article  Google Scholar 

  20. Zhou, Y., He, Z.H., Sui, X.X., Xia, X.C., Zhang, X.K., Zhang, G.S. 2007. Genetic improvement of grain yield and associated traits in the Northern China winter wheat region from 1960 to 2000. Crop Sci. 47:245–253.

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to L. Purnhauser.

Additional information

Communicated by J. Kolmer

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Cite this article

Purnhauser, L., Bóna, L. & Láng, L. Identification of Sr31 and Sr36 stem rust resistance genes in wheat cultivars registered in Hungary. CEREAL RESEARCH COMMUNICATIONS 39, 53–66 (2011). https://doi.org/10.1556/CRC.39.2011.1.6

Download citation

Keywords

  • 1AL.RS
  • 1BL.1RS
  • microsatellite
  • molecular marker
  • SSR
  • Ug99