Cereal Research Communications

, Volume 39, Issue 1, pp 1–11 | Cite as

RT-PCR detection of the expression of COP1 and SINAT5 E3 ubiquitin ligase genes in different organs of Zea mays L.

  • A. GholizadehEmail author
  • B. Kohnehrouz
Open Access


Using bioinformatic data, we found that maize homologues of the Arabidopsis single subunit RING type ubiquitin ligase genes, COP1 (Constitutive Photomorphogenesis 1) and SINAT5 (Seven in Absentia in Arabidopsis thaliana 5) have been frequently predicted during large-scale cDNA sequencing project and released to databases since 2008. Despite this general information, no tissue specific expression profiles of these genes were published to our knowledge so far. In the present research, the expression and the relative levels of COP1 and SINAT5 transcripts were detected using reverse transcription and polymerase chain reaction (RT-PCR) with template materials collected separately from three different organs (root, leaf and seed) of maize plants grown under the normal growth conditions. The results not only confirmed the presence of COP1 transcript in all three test samples but also revealed its abundance in root tissues that may be consistent to its large number of targets for ubiquitination in darkness. Analysis of SINAT5 expression profile revealed a detectable band in leaf tissue sample that may be related to its specific roles in this organ.


expression profile E3 ubiquitin ligase COP1 SINAT5 maize 


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© Akadémiai Kiadó, Budapest 2011

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Institute for Fundamental Sciences (RIFS)University of Tabriz ResearchTabrizIran
  2. 2.Department of Plant Breeding and BiotechnologyUniversity of TabrizTabrizIran

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