The effect of amino acids on nitrate uptake by wheat roots


To identify feedback signal(s) controlling nitrate uptake by wheat roots, the effects of 13 amino acids on time course of net nitrate influx have been investigated. A new non-invasive technique of measuring net nitrate influx with a high time resolution of minutes has enabled us to make this comparison. The results showed that glutamate caused a rapid and large decrease of net nitrate influx by roots. Aspartic acid, asparagine, glutamine caused a relatively slow decrease of net nitrate influx with a lag of 0.5–1.5 h. Other amino acids had a small effect or even no effect. It is speculated that glutamate is most likely to be a negative feedback signal.


  1. Beuve, N., Rispail, N., Laine, P., Cliquet, J.B., Ourry A., Ledeunff, E. 2004. Putative role of-aminobutyric acid (GABA) as a long-distance signal in up-regulation of nitrate uptake in Brassica napus L. Plant Cell Environ. 27:1035–1046.

    CAS  Article  Google Scholar 

  2. Cram, W.J. 1976. Negative feedback regulation of transport in cells. The maintenance of turgor, volume and nutrient supply. In: Lüttge, U., Pitman, M.G. (eds), Encyclopedia of Plant Physiology, New Series, Vol. II A. Springer Verlag, Berlin, Germany. pp. 284–316.

    Google Scholar 

  3. Fan, X.R., Weeks, R.G., Shen, Q.R., Miller, A.J. 2006. Glutamine transport and feedback regulation of nitrate reductase activity in barley roots leads to changes in cytosolic nitrate pools. J. Exp. Bot. 57:1333–1340.

    CAS  Article  Google Scholar 

  4. Forde, B.G., Lea, P.J. 2007. Glutamate in plants: Metabolism, regulation, and signaling. J. Exp. Bot. Doi:10.1093/jxb/erm121.

  5. Geßler, A., Kopriva, S., Rennenberg, H. 2004. Regulation of nitrate uptake at the whole-tree level: Interaction between nitrogen compounds, cytokinins and carbon metabolism. Tree Physiol. 24:1313–1321.

    Article  Google Scholar 

  6. Ivashikina, N.V., Sokolov, O.A. 1997. Regulation of nitrate uptake and distribution in maize seedlings by nitrate, nitrite, ammonium and glutamate. Plant Sci. 123:29–37.

    CAS  Article  Google Scholar 

  7. Lam, H.M., Chiao, Y.A., Li, M.W., Yung, Y.K., Ji, S. 2006. Putative nitrogen sensing systems in higher plants. Journal of Integrative Plant Biology 48:873–888.

    CAS  Article  Google Scholar 

  8. Padgett, P.E., Leonard, R.T. 1996. Free amino acid levels and the regulation of nitrate uptake in maize cell suspension cultures. J. Exp. Bot. 47:871–883.

    CAS  Article  Google Scholar 

  9. Stitt, M., Müller, C., Matt, P., Gibon, Y., Carillo, P., Morcuende, R., Scheible, W.R., Krapp, A. 2002. Steps towards an integrated view of nitrogen metabolism. J. Exp. Bot. 53:959–970.

    CAS  Article  Google Scholar 

  10. Toates, F.M. 1975. Control Theory in Biology and Experimental Psychology. Hutchinson Educational, London, UK.

    Google Scholar 

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Correspondence to J. Z. Li.

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Communicated by J. Zhang

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Li, J.Z., He, G.Y. & Cram, W.J. The effect of amino acids on nitrate uptake by wheat roots. CEREAL RESEARCH COMMUNICATIONS 38, 482–488 (2010).

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  • amino acids
  • nitrate uptake
  • non-invasive
  • signal
  • Triticum aestivum L.