Length polymorphism at the Glu-A3 and Glu-D3 in wild relatives of wheat

Abstract

Low molecular weight glutenin subunits are important components of wheat seed storage protein, and play a significant in determining the end-use quality characteristics of wheat varieties. Allelic variation of the LMW-GS is associated with the significant differences of dough quality in bread and durum wheat, and has been widely evaluated at protein level in wheat and its relatives. In this study seven specific primers, specifically amplify genes located at the Glu-A3 and Glu-D3 loci, were employed to assay the length variation of LMW-GS genes in the A and D-genomes of diploid wheats. A total of 86 accessions of diploid wheats, including 10 accessions of T. boeoticum and 76 accessions of Ae. tauschii, were investigated. Seven alleles were detected in accessions of T. boeoticum (Glu-A3 locus) by two pairs of specific primers and eighteen alleles were detected in accessions of Ae. tauschii (Glu-D3 locus) by five pairs of specific primers. A higher level of allelic variation of LMW-GS was found in accession T. boeoticum and Ae. tauschii with Nei’s genetic variation index (H) of 0.82 and 0.92, respectively. This allelic variation could be used as valuable source for the enrichment of genetic variations and the alteration of flour-processing properties of the cultivated wheat.

References

  1. Appelbee, M.J., Mekuria, G.T., Nagasandra V., Bonneau, J.P., Eagles, H.A., Eastwood, R.F., Mather, D.E. 2009. Novel allelic variants encoded at the Glu-D3 locus in bread wheat. Cereal Sci. 30: 1–8.

    Google Scholar 

  2. Atienza, S.G., Alvarez, J.B., Villegas, A.M. 2002. Variation for the low-molecular-weight glutenin subunits in a collection of Hordeum chilense. Euphytica 128: 269–277.

    CAS  Article  Google Scholar 

  3. Bietz, J.A., Wall, J.S. 1973. Isolation and characterization of gliadin-like subunits from glutenins. Cereal Chem. 50: 537–547.

    CAS  Google Scholar 

  4. Cassidy, B.G., Dvorak, J. 1991. Molecular characterization of a low-molecular-weight glutenin cDNA clone from Triticum durum. Theor. Appl. Genet. 81: 653–660.

    CAS  Article  Google Scholar 

  5. Cassidy, B.G., Dvorak, J., Anderson, O.D. 1998. The wheat low-molecular-weight glutenin genes: Characterization of six new genes and progress in understanding gene family structure. Theor. Appl. Genet. 96: 743–750.

    CAS  Article  Google Scholar 

  6. D’Ovidio, R., Simeone, M., Masci, S., Porceddu, E. 1997. Molecular characterization of a LMW-GS gene located on chromosome 1B an the development of primers specific for the Glu-B3 complex locus in durum wheat. Theor. Appl. Genet. 95: 1119–1126.

    Article  Google Scholar 

  7. D’Ovidio, R., Masci, S. 2004. The low-molecular-weight glutenin subunits of wheat gluten. J. Cereal Sci. 39: 321–339.

    Article  Google Scholar 

  8. D’Ovidio, R., Marchitelli, C., Cardelli, L.E., Porceddu, E. 1999. Sequence similarity between allelic Glu-B3 genes related to quality properties of durum wheat. Theoretical and Applied Genetics 98: 455–461.

    Article  Google Scholar 

  9. Gianibelli, M.C., Larroque, O.R., MacRitchie, F., Wrigley, C.W. 2001. Biochemical, genetic, and molecular characterization of wheat glutenin and its component subunits. Cereal Chem. 78: 635–646.

    CAS  Article  Google Scholar 

  10. Gupta, R.B., Shepherd, K.W. 1988. Low-molecular-weight glutenin subunits in wheat: Their variation, inheritance and association with bread-making quality. In: Miller, T.E., Koebner, R.M.D. (eds), Proceedings of the 7th International Wheat Genetics Symposium. England, Cambridge, pp. 943–949.

  11. Gupta, R.B., Paul, J.G., Cornish, G.B. 1994. Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3, and Gli-1 of common wheats. I. Its additive and interaction effects on dough properties. J. Cereal Sci. 19: 9–17.

    CAS  Article  Google Scholar 

  12. Gupta, R.B., Shepherd, K.W. 1990. Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutenin. 1. Variation and genetic control of the subunits in hexaploid wheats. Theor. Appl. Genet. 80: 65–74.

    CAS  Article  Google Scholar 

  13. Gupta, R.B., Singh, N.D., Shepherd, K.W. 1989. The cumulative effect of allelic variation in LMW and HMW glutenin subunits on physical dough properties in progeny of two bread wheats. Theor. Appl. Genet. 77: 57–64.

    CAS  Article  Google Scholar 

  14. Haung, Z., Long, H., Yan, Z.H. 2005. Sequence analysis of LMW-GS genes from Aegilops tauschii containing HMW glutenin subunits 5+12 with good quality. High Technol. Letter 15: 67–72.

    Google Scholar 

  15. Ikeda, T.M., Nagamine, T., Fukuoka, H., Yano, H. 2002. Identification of new low-molecular-weight glutenin subunit genes in wheat. Theor. Appl. Genet. 104: 680–687.

    CAS  Article  Google Scholar 

  16. Jackson, E.A., Holt, L.M., Payne, P.I. 1983. Characterization of high molecular weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two-dimensional electrophoresis and the chromosomal localization of their controlling genes. Theor. Appl. Genet. 66: 29–37.

    CAS  Article  Google Scholar 

  17. Lee, Y.K., Bekes, F., Gupta, R. 1999. The low-molecular-weight glutenin subunit proteins of primitive wheats: I. Variation in A-genome species. Theor. Appl. Genet. 98: 119–125.

    CAS  Article  Google Scholar 

  18. Long, H., Huang, Z., Wei, Y.M., Yan, Z.H., Ma, Z.C., Zheng, X.L. 2008. Length variation of i-type low-molecular-weight glutenin subunit genes in diploid wheats. Russian J. Genetics 44: 429–435.

    CAS  Article  Google Scholar 

  19. Long, H., Wei, Y.M., Yan, Z.H. 2005. Classification of wheat low-molecular weight glutenin subunit genes and its chromosome assignment by developing LMWGS group-specific primers. Theor. Appl. Genet. 111: 1251–1259.

    CAS  Article  Google Scholar 

  20. Long, H., Wei, Y.M., Yan, Z.H., Zheng, Y.L. 2004. Molecular cloning of a novel low-molecular-weight glutenin subunit gene from wheat (Triticum aestivum L.) variety Chuannong 16. Acta Agronomica Sinica 30: 1179–1184.

    CAS  Google Scholar 

  21. Ma, Z.C., Wei, Y.M., Long, H. 2006. Characterization of low-molecular-weight i-type glutenin subunit genes from diploid wheat in relation to the gene family structure. Mol. Biol. 40: 897–906.

    CAS  Article  Google Scholar 

  22. Masci, S., Rovelli, L., Kasarda, D.D. 2002. Characterization and chromosomal localization of C-type low-molecular-weight glutenin subunits in the bread wheat cultivar Chinese Spring. Theor. Appl. Genet. 104: 422–428.

    CAS  Article  Google Scholar 

  23. Murray, M., Thompson, W.F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8: 4321–4325.

    CAS  Article  Google Scholar 

  24. Nagamine, T., Kai, Y., Takayama, T. 2000. Allelic variation at the Glu-1 and Glu-3 loci in southern Japanese wheats, and its effects on gluten properties. J. Cereal Sci. 32: 129–135.

    CAS  Article  Google Scholar 

  25. Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl Acad. Sci. USA 70: 3321–3323.

    CAS  Article  Google Scholar 

  26. Osborne, T.B. 1924. The Vegetable Proteins. 2nd edn. Longmans, Green and Co., London.

    Google Scholar 

  27. Pogna, N.E., Autran, J.C., Mellini, F. 1990. Chromosome 1B-encoded gliadins and glutenin subunits in durum wheat: genetics and relationship to gluten strength. J. Cereal Sci. 11: 15–34.

    CAS  Article  Google Scholar 

  28. Ruiz, M., Carrillo, J.M., 1993. Linkage relationships between prolamin genes on chromosomes 1A and 1B of durum wheat. Theor. Appl. Genet. 87: 353–360.

    CAS  Article  Google Scholar 

  29. Shewry, P.R., Halford, N.G., Tatham, A.S. 1989. The high molecular weight subunits of wheat, barley and rye, genetics, molecular biology, chemistry and role in wheat gluten structure and functionality. In: Miflin, B.J. (ed.), Oxford Survey Plant Molecular and Cellular Biology. Oxford University Press, New York, Vol. 6, pp. 163–219.

    Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to M. R. Bihamta.

Additional information

Communicated by F. Békés

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Cite this article

Khoshro, H.H., Bihamta, M.R., Hassanii, M.E. et al. Length polymorphism at the Glu-A3 and Glu-D3 in wild relatives of wheat. CEREAL RESEARCH COMMUNICATIONS 38, 375–385 (2010). https://doi.org/10.1556/CRC.38.2010.3.8

Download citation

Keywords

  • length polymorphism
  • specific markers
  • LMW-GS
  • diploid wheat