Physiologic specialization of wheat leaf rust (Puccinia triticina Eriks.) in the Czech Republic in 2005–2008


In 2005–2008 virulence of the wheat leaf rust population was studied on Thatcher near-isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr10, Lr11, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust were obtained from different parts of the Czech Republic. A total of 233 wheat leaf rust isolates were analyzed. Resistance gene Lr9 was effective to all tested isolates as in the four previous years. Virulence to Lr19 (0.8% of the analysed isolates) was found. Gene Lr24 conditioned resistance to almost all collections and a lower frequency of virulence was also observed on Lr2a and Lr28. Twenty-six winter wheat cultivars, seven spring wheat cultivars and seven winter triticale cultivars registered in 2005–2008 were tested with 7 leaf rust isolates. Winter wheat cultivars Biscay, Mulan and Orlando were resistant to all isolates and were also resistant in field trials.


  1. Bartoš, P., Šebesta, J. 1971. Physiologic specialization of Puccinia recondita Rob. ex Desm. f. sp. tritici (Eriks) in Czechoslovakia in the years 1966–1969. Genetika a štechtǐní 7: 23–28. (In Czech)

    Google Scholar 

  2. Bradová, J., Šašek, A. 2007. Optimalizace metod elektroforézy proteinù pro identifikaci odrùd pšenice (Triticum aestivum L.). (Optimization of methods of protein electrophoresis for the identification of wheat cultivars). VÚRV, v.v.i., Praha, 2007, 36 pp. ISBN 978-80-87011-10-2. (In Czech)

    Google Scholar 

  3. Goyeau, H., Park, R., Schaeffer, B., Lannou, C. 2006. Distribution of pathotypes with regard to host cultivars in French wheat leaf rust populations. Phytopathol. 96: 264–273.

    CAS  Article  Google Scholar 

  4. Gultyaeva, E.I. 2007. Wheat Leaf Rust Survey in Russia in 2001–2006. XV. Congress of European Mycologists, Sankt Peterburg, September 16–21, 2007, Abstracts, p. 252.

  5. Gultyaeva, E., Walter, U., Kopahnke, D., Mikhailova, L. 2000. Virulence of Puccinia recondita Rob. ex Desm. f. sp. tritici in Germany and European part of Russia in 1996–1999. Acta Phytopathologica et Entomologica Hungarica 35: 409–412.

    Google Scholar 

  6. Hanzalová, A., Bartoš, P. 2006. Physiologic specialization of wheat leaf rust (Puccinia triticina Eriks.) in the Czech Republic in 2001–2004. Czech J. Genet. Plant Breed. 42: 126–131.

    Article  Google Scholar 

  7. Hanzalová, A., Sumíková, T., Bartoš, P. 2009. Determination of leaf rust resistance genes Lr10, Lr26 and Lr37 by molecular markers in wheat cultivars registered in the Czech Republic. Czech J. Genet. Plant Breed. 45: 79–84.

    Article  Google Scholar 

  8. Liatukas, Z. 2003. Virulence of winter wheat leaf rust isolates. Biologija (Lithuania) 1: 77–80.

    Google Scholar 

  9. Limpert, E., Müller, K. 1994. Designation of pathotypes of plant pathogens. J. Phytopathol. 140: 364–358.

    Google Scholar 

  10. Lind, V., Gultyaeva, R. 2007. Virulence frequencies of Puccinia triticina in Germany and the European regions of the Russian Federation. J. Phytopathol. 155: 13–21.

    Article  Google Scholar 

  11. Manninger, K. 2006. Physiological specialization of Puccinia triticina on wheat and triticale in Hungary in 2004. Acta Phytopathologica et Entomologica Hungarica 41: 93–100.

    Article  Google Scholar 

  12. Martynov, S.P., Dobrotvorskaya, I.V. 2006. Wheat-pedigree and identified alleles of genes.

  13. McIntosh, R.A., Wellings, C.R., Park, R.H. 1995. Wheat Rusts. An Atlas of Resistance Genes. CSIRO, Australia.

    Google Scholar 

  14. Mesterházy, A., Bartoš, P., Goyeau, H., Niks, R., Csösz, M., Andersen, O., Casulli, F., Ittu, M., Jones, E., Manisterski, J., Manninger, K., Pasquini, M., Rubiales, D., Schachermayr, G., Strzembicka, A., Szunics, L., Todorova, M., Unger, O., Vanco, B., Vida, G., Walther, U. 2000. European virulence survey for leaf rust in wheat. Agronomie 20: 793–804.

    Article  Google Scholar 

  15. Quinones, M.A., Larter, E.N., Samborski, D.J. 1972. The inheritance of resistance to Puccinia recondita in hexaploid triticale. Canadian J. of Genetics and Cytology 14: 495–505 (cit. after McIntosh et al. 1995).

    Article  Google Scholar 

  16. Stakman, E.C., Stewart, P.M., Loegering, W.O. 1962. Identification of Physiologic Races of Puccinia graminis var. tritici. Agricultural Research Service E617. United States Department of Agriculture: Washington DC, USA.

    Google Scholar 

  17. Šebesta, J., Bartoš, P. 1968. Physiologic specialization of wheat leaf rust (Puccinia recondita Rob. ex Desm. f. sp. tritici /Eriks./) in Czechoslovakia in the years 1962 and 1963. Ochrana Rostlin 4: 1–8. (In Czech)

    Google Scholar 

  18. Šebesta, J., Bartoš, P. 1969. Physiologic specialization of wheat leaf rust in Czechoslovakia during 1964 and 1965. Ochrana Rostlin 5: 147–154. (In Czech)

    Google Scholar 

  19. Winzeler, M., Mesterházy, A., Park, R.F., Bartoš, P., Csösz, M., Goyeau, H., Ittu, M., Jones, E., Löschenberger, F., Manninger, K., Pasquini, M., Richter, K., Rubiales, D., Schachermayr, G., Strzembicka, A., Trottet, M., Unger, O., Vida, G., Walther, U. 2000. Resistance of European winter wheat germplasm to leaf rust. Agronomie 20: 783–792.

    Article  Google Scholar 

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Correspondence to A. Hanzalová.

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Communicated by J. Kolmer

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Hanzalová, A. Physiologic specialization of wheat leaf rust (Puccinia triticina Eriks.) in the Czech Republic in 2005–2008. CEREAL RESEARCH COMMUNICATIONS 38, 366–374 (2010).

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  • leaf rust pathotypes
  • Lr genes
  • wheat
  • triticale
  • resistance