Evaluation of seedling survivability and growth response as selection criteria for breeding drought tolerance in wheat

Abstract

Detection of genotypic variation in response to water stress at seedling stage could help in escalating selection intensity in breeding drought tolerant varities. Nine genotypes were tested for seedling survivability under drought stress. Four genotypes, i.e. ‘Sarsabz’, ‘Sitta’, ‘Fareed’ and ‘FD-83’, showed complete survival on resumption of irrigation after drought stress. These genotypes were late dying as they withered slowly under drought. Percent wilting and percent survival on resumption of irrigation were negatively correlated. Six genotypes were selected on the basis of seedling survivability (late and early dying) and evaluated for seedling growth response under drought. Root length and dry weight increased significantly under stress in ‘Sitta’, ‘FD-83’ and ‘Fareed’. Drought stress also increased the root-to-shoot length ratio in ‘FD-83’ and ‘Fareed’. However, seedling fresh and dry weight significantly reduced in ‘Nesser’ and ‘Inqalab-91’ under stress. In ‘FD-83’, seedling fresh and dry weight increased over control under stress. Results indicated that seedling survivability, root-to-shoot length ratio, root length and dry weight were most important traits for screening drought tolerance at seedling stage. On the basis of these indices, ‘Sitta’, ‘Fareed’ and ‘FD-83’ were classified as drought tolerant, ‘Sarsabz’ and ‘Nesser’ as moderately tolerant and ‘Inqalab-91’ as sensitive genotypes. Collectively, results suggested that selection by combining seedling survivability, growth response, RWC and leaf water potential can be efficiently used for rapid evaluation of drought tolerance in wheat breeding.

References

  1. Akhter, J., Sabir, S.A., Lateef, Z., Ashraf M.Y., Haq, M.A. 2008. Relationship between carbon isotope discrimination and grain yield, water use efficiency and growth parameters in wheat (Triticum aestivum L.) under different water regimes. Pak. J. Bot. 40:1441–1454.

    Google Scholar 

  2. Bajji, M., Lutts, S., Kinet, J.M. 2000. Physiological changes after exposure to and recovery from polyethylene glycol-induced water deficit in roots and leaves of durum wheat (Triticum durum Desf.) cultivars differing in drought resistance. J. Plant Physiol. 157:100–108.

    CAS  Article  Google Scholar 

  3. Basal, H., Smith, C.W., Thaxton, P.S., Hemphill, J.K. 2005. Seedling drought tolerance in upland cotton. Crop Sci. 45:766–771.

    Article  Google Scholar 

  4. Bray, E.A. 1997. Plant responses to water deficit. Trends Plant Sci. 2:48–54.

    Article  Google Scholar 

  5. Dash, S., Mohanty, N. 2001. Evaluation of assays for the analysis of thermotolerance and recovery potentials of seedlings of wheat (Triticum aestivum L.) cultivars. J. Plant Physiol. 158:1153–1165.

    CAS  Article  Google Scholar 

  6. Dhanda, S.S., Sethi, G.S., Behl, R.K. 2004. Indices of drought tolerance in wheat genotypes at early stages of plant growth. J. Agron. Crop Sci. 190:6–12.

    Article  Google Scholar 

  7. Ezzat-Ollah, E., Shakiba, M.R., Mahboob, S.A. 2007. Water stress, antioxidant enzyme activity and lipid peroxidation in wheat seedling. Int. J. Food Agri. Environ. 5:149–153.

    Google Scholar 

  8. HongBo, S., ZongSuob L., MingAn, S. 2006. Osmotic regulation of 10 wheat (Triticum aestivum L.) genotypes at soil water deficits. Colloids and Surfaces B: Biointerfaces 47:132–139.

    Article  Google Scholar 

  9. Kaydan, D., Yagmur, M. 2008. Germination, seedling growth and relative water content of shoot in different seed sizes of triticale under osmotic stress of water and NaCl. African J. Biotech. 7:2862–2868.

    CAS  Google Scholar 

  10. Leishman, M.R., Westoby, M. 1994. The role of seed size in seedling establishment in dry soil conditions-experimental evidence from semi-arid species. J. Ecol. 82:249–258.

    Article  Google Scholar 

  11. Matsui, T., Singh, B.B. 2003. Root characteristics in cowpea related to drought tolerance at the seedling stage. Experimental Agriculture 39:29–38.

    Article  Google Scholar 

  12. McMichael, B.L., Quisenberry, J.E. 1991. Genetic variation for root-shoot relationship among cotton germplasm. Environ. Exp. Bot. 31:461–470.

    Article  Google Scholar 

  13. Mitchell, J.H., Siamhan, D., Wamala, M.H., Risimeri, J.B., Chinyamakobvu, E., Henderson, S.A., Fukai, S. 1998. The use of seedling leaf death score for evaluation of drought resistance of rice. Field Crops Res. 55:129–139.

    Article  Google Scholar 

  14. Nagarajan, S., Rane, J. 2000. Relationship of seedling traits with drought tolerance in spring wheat cultivars. Indian J. Plant Physiol. 5:264–270.

    Google Scholar 

  15. Ren, H., Chen, X., Sun, G., Wang, Y. 2000. Response of wheat seedlings with different drought resistance to water deficiency and NaCl stresses. Ying Yong Sheng Tai Xue Bao 11:718–722.

    CAS  PubMed  Google Scholar 

  16. Schonfeld, M.A., Johnson, R.C., Carver, B.F., Mornhigweg, D.W. 1988. Water relations in winter wheat as drought resistance indicators. Crop Sci. 28:526–531.

    Article  Google Scholar 

  17. Selote, D.S., Bharti, S., Khanna-Chopra, R. 2004. Drought acclimation reduces O−2 accumulation and lipid peroxidation in wheat seedlings. Biochem. Bioph. Res. Co. 314:724–729.

    CAS  Article  Google Scholar 

  18. Siddiqui, S.U., Ali, A., Chaudhary, M. F. 2008. Germination behavior of wheat (Triticum aestivum L.) varities to artificial ageing under varying temperature and humidity. Pak. J. Bot. 40:1121–1127.

    Google Scholar 

  19. Sinclair, T.R., Ludlow, M.M. 1985. Who taught plant thermodynamics? The unfulfilled potential of plant water potential. Aust. J. Plant Physiol. 12:213–217.

    Google Scholar 

  20. Singh, B.B., Mai-Kodomi, Y., Terao, T. 1999. A simple screening method for drought tolerance in cowpea. Indian J. Genet. 59:211–220.

    Google Scholar 

  21. Soltani, A., Gholipoor, M., Zeinali, E. 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environ. Exp. Bot. 55:195–200.

    Article  Google Scholar 

  22. Subrahmanyam, D., Subash, N., Haris, A., Sikka, A.K. 2006. Influence of water stress on leaf photosynthetic characteristics in wheat cultivars differing in their susceptibility to drought. Photosynthetica 44:125–129.

    CAS  Article  Google Scholar 

  23. Tomar, S.M.S., Kumar, G.T. 2004. Seedling survivability as a selection criterion for drought tolerance in wheat. Plant Breeding 123:392–394.

    Article  Google Scholar 

  24. Yang, R.C., Jana, S., Clarke, J.M. 1991. Phenotypic diversity and association of some potentially drought-responsive characters in drum wheat. Crop Sci. 31:1484–1491.

    Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to A. Hameed.

Additional information

Communicated by A. Pécsváradi

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Cite this article

Hameed, A., Goher, M. & Iqbal, N. Evaluation of seedling survivability and growth response as selection criteria for breeding drought tolerance in wheat. CEREAL RESEARCH COMMUNICATIONS 38, 193–202 (2010). https://doi.org/10.1556/CRC.38.2010.2.5

Download citation

Keywords

  • biomass
  • root length
  • root-to-shoot ratio
  • Triticum aestivum L.
  • water deficit