Cereal Research Communications

, Volume 38, Issue 2, pp 155–162 | Cite as

Identification of durum wheat cultivars by a minimum number of microsatellite markers

  • G. ManginiEmail author
  • F. Taranto
  • S. L. Giove
  • A. Gadaleta
  • A. Blanco


A quick and reproducible tool for cultivar identification is useful to assess in certified seed production programs and to resolve legal conflicts. Simple Sequence Repeats (SSRs) have been the elected markers to carry out cultivar identification studies. The main aim of this research was to define the minimum number of SSR markers to distinguish 80 durum wheat cultivars. Preliminary, an analysis of 11 SSRs informativeness was carried out on a subset of 28 durum wheat cultivars. The discriminating ability of each primer was estimated both with Polymorphism Information Content (PIC) and with Resolving power (Rp). Rp resulted the best parameter for assessing the discriminatory power of SSR primers (r=0.94***; P≤0.001). The marker Xwmc597 was able to discriminate all the 28 cultivars. Successively, 80 genotypes were analysed using three SSR markers with the higher Rp value. Two SSRs were able to distinguish all the 80 genotypes. Particularly, Xwmc597 was able to distinguish 69/80 genotypes while Xwmc415 identified the other cultivars. An identification key was obtained combining the data of these two markers.


cultivar identification durum wheat identification key microsatellite markers 


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  1. Agarwal, M., Shrivastava, N., Padh, H. 2008. Advances in molecular marker techniques and their applications in plant sciences. Plant Cell Rep. 27:617–631.CrossRefGoogle Scholar
  2. Anderson, J.A., Churchill, G.A., Sutrique, J.E., Tanksley, S.D., Sorrells, M.E. 1993. Optimizing parental selection for genetic linkage maps. Genome 36:181–186.CrossRefGoogle Scholar
  3. Di Fonzo, N., Ravaglia, S., DeAmbrogio, E., Blanco, A., Troccoli, A. 2005. Durum Wheat Improvement in Italy. In: Royo, C., Nachit, M.M., Di Fonzo, N., Araus, J.L., Pfeiffer, W.H., Slafer, G.A. (eds), Durum Wheat Breeding: Current Approaches and Future Strategies. The Haworth Press, Binghamton, NY, pp. 825–881.Google Scholar
  4. Escandón, A.S., Zelener, N., Pérez de la Torre, M., Soto, S. 2007. Molecular identification of new varieties of Nierembergia linariaefolia (Graham), a native Argentinean ornamental plant. J. Appl. Genet. 48:115–123.CrossRefGoogle Scholar
  5. Fernández, M.E., Figueras, A.M., Benito, C. 2002. The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetic diversity among barley cultivars with known origin. Theor. Appl. Genet. 104:845–851.CrossRefGoogle Scholar
  6. Gadaleta, A., Mangini, G., Mulè, G., Blanco, A. 2007. Characterization of dinucleotide and trinucleotide EST-derived microsatellites in the wheat genome. Euphytica 153:73–85.CrossRefGoogle Scholar
  7. García-Martínez, S., Andreani, L., Garcia-Gusano, M., Geuna, F., Ruiz, J.J. 2006. Evaluation of amplified fragment length polymorphism and simple sequence repeats for tomato germplasm fingerprinting: Utility for grouping closely related traditional cultivars. Genome 49:648–656.CrossRefGoogle Scholar
  8. Gupta, P.K., Balyan, H.S., Edwards, K.J., Isaac, P., Korzun, V., Röder, M.S., Gautier, M.F., Joudrier, P., Schlatter, A.R., Dubcovsky, J., De la Pena, R.C., Khairallah, M., Penner, G., Hayden, M.J., Sharp, P., Keller, B., Wang, R.C.C., Hardouin, J.P., Jack, P., Leroy, P. 2002. Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor. Appl. Genet. 105:413–422.CrossRefGoogle Scholar
  9. Huang, X.Q., Börner, A., Röder, M.S., Ganal, M.W. 2002. Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers. Theor. Appl. Genet. 105:699–707.CrossRefGoogle Scholar
  10. Li, Y.C., Korol, A.B., Fahima, T., Beiles, A., Nevo, E. 2002. Microsatellites: genomic distribution, putative functions and mutational mechanism (a review). Mol. Eco. 11:2543–2565.CrossRefGoogle Scholar
  11. Maccaferri, M., Sanguineti, M.C., Donini, P., Porceddu, E., Tuberosa, R. 2005. A retrospective analysis of genetic diversity in durum wheat elite germplasm based on microsatellite analysis: A case study. In: Royo, C., Nachit, M.M., Di Fonzo, N., Araus, J.L., Pfeiffer, W.H., Slafer, G.A. (eds), Durum Wheat Breeding: Current Approaches and Future Strategies. The Haworth Press, Binghamton, NY. pp. 99–142Google Scholar
  12. Perry, J.D. 2004. Identification of Canadian durum wheat varieties using a single PCR. Theor. Appl. Genet. 109:55–61.CrossRefGoogle Scholar
  13. Prasad, M., Varsheney, R.K., Balyan, H.S., Gupta, P.K. 2000. The use of microsatellites for detecting DNApolymorphism, genotype identification and genetic diversity in wheat. Theor. Appl. Genet. 100:584–592.Google Scholar
  14. Prevost, A., Wilkinson, M.J. 1999. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor. Appl. Genet. 98:107–112.CrossRefGoogle Scholar
  15. Röder, M.S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M.H., Leroy, P., Ganal, M.W. 1998. A microsatellite map of wheat. Genetics 149:2007–2023.PubMedPubMedCentralGoogle Scholar
  16. Somers, J.D., Isaac, P., Edwards, K. 2004. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 109:1105–1114.CrossRefGoogle Scholar
  17. Somers, J.D., Banks, T., DePauw, R., Fox, S., Clarke, J., Pozniak, C., McCarteney, C., 2007. Genome-wide linkage disequilibrium analysis in bread wheat and durum wheat. Genome 50:557–567.CrossRefGoogle Scholar
  18. Tatikonda, L., Wani, S.P., Kannan, S., Beerelli, N., Sreedevi, T.K., Hoisington, D.A., Devi, P., Varshney, R.K. 2009. AFLP-based molecular characterization of an elite germplasm collection of Jatropha curcas L., a biofuel plant. Plant Sci. 176:505–513.CrossRefGoogle Scholar
  19. Varshney, R.K., Chabane, K., Hendre, P.S., Aggarwal, R.K., Graner, A. 2007. Comparative assessment of EST-SSR, EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleys. Crop Sci. 173:638–649.Google Scholar
  20. Varshney, R.K., Graner, A., Sorrells, M.E. 2005. Genic microsatellite markers: Features and applications. Trends Biotechnol. 23:48–55.CrossRefGoogle Scholar
  21. Zane, L., Bargelloni, L., Patarnello, T. 2002. Strategies for microsatellite isolation: Areview. Mol. Eco. 11:1–16.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2010

Authors and Affiliations

  • G. Mangini
    • 1
    Email author
  • F. Taranto
    • 1
  • S. L. Giove
    • 1
  • A. Gadaleta
    • 1
  • A. Blanco
    • 1
  1. 1.Department of Agro-Forestry and Environmental Biology and ChemistryUniversity of BariBariItaly

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