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Cereal Research Communications

, Volume 38, Issue 1, pp 67–74 | Cite as

The nucleotide sequence of barley strain of Wheat dwarf virus isolated in Hungary

  • I. TóbiásEmail author
  • B. Kiss
  • K. Salánki
  • L. Palkovics
Open Access
Pathology

Abstract

Barley-infecting isolates of Wheat dwarf virus (WDV) were collected in the field in the vicinity of the cities Dunakiliti, Heves and Siófok, in Hungary. Viral genomic DNA was amplified by the rolling circle amplification technique, digested with HindIII, cloned into pBSK+ plasmid and sequenced. The clones were of the same size and showed above 99% identity to each other. Based on DNA sequences WDV-D01, WDV-H1 and WDV-H07 isolates showed high identity (94–99%) to isolates of WDV barley strain and Barley dwarf virus and lower identity to Oat dwarf virus (71% identity) and WDV wheat strains (85% identity).

Keywords

Wheat dwarf virus Barley dwarf virus Oat dwarf virus barley-infecting isolates sequence analysis 

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Copyright information

© Akadémiai Kiadó, Budapest 2010

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • I. Tóbiás
    • 1
    Email author
  • B. Kiss
    • 1
  • K. Salánki
    • 2
  • L. Palkovics
    • 3
  1. 1.Plant Protection InstituteHungarian Academy of SciencesBudapestHungary
  2. 2.Agricultural Biotechnology CenterGödöllőHungary
  3. 3.Department of Plant PathologyCorvinus University of BudapestBudapestHungary

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