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Cereal Research Communications

, Volume 38, Issue 1, pp 1–14 | Cite as

Characterization and classification of γ-gliadin multigene sequences from Aegilops section Sitopsis

  • Z. Huang
  • H. Long
  • Y. M. WeiEmail author
  • P. F. Qi
  • Z. H. Yan
  • Y. L. ZhengEmail author
Genetics

Abstract

The most abundant seed storage proteins of wheat are gliadins and glutenins. Gliadins, including α/β, γ and ω types, are normally monomeric proteins and account for about 50% of the gluten proteins. In this study, 55 sequences of γ-gliadin genes were obtained from species of Sitopsis section, the deduced B genome donors of wheat. Despite the high sequence similarities to the known γ-gliadin genes, extensive variations were also found. Using the extensive sequence information deposited in database and obtained in this study, a comprehensive classification of the γ-gliadin multigene families were performed based on the primary structures and phylogenic analysis. All the γ-gliadin genes analyzed could be divided into 2 types, which contain 8 and 9 cysteines, respectively. Type I (with 8 cysteines) and type II (with 9 cysteines) are further classified to 7 and 4 groups, respectively, and several subgroups are also identified. The genes derived from A, B and D genomes of common wheat were clustered distinctly, indicating that there was apparent genomic specificity in γ-gliadins genes. Besides the high homology between γ-gliadin genes from Sitopsis species and B genome of wheat, some unique groups or subgroups were also identified in Sitopsis section, suggesting that it could be considered as a valuable source of γ-gliadin genes. The comparison of deduced primary structures of each group and/or subgroup was conducted, from which their evolution and quality properties were also speculated.

Keywords

wheat Aegilops seed storage protein γ-gliadin 

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References

  1. Anderson, O.D., Greene, F.C. 1989. The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat. Theor. Appl. Genet. 77: 689–700.CrossRefGoogle Scholar
  2. Anderson, O.D., Hsia, C.C., Torres, V. 2001. The wheat γ -gliadin genes: Characterization of ten new sequences and further understanding of gamma-gliadin gene family structure. Theor. Appl. Genet. 103: 323–330.CrossRefGoogle Scholar
  3. Arentz-Hansen, E.H., McAdam, S.N., Molberg, O., Kristiansen, C., Sollid, L.M. 2000. Production of a panel of recombinant gliadins for the characterisation of T cell reactivity in coeliac disease. Gut 46: 46–51.CrossRefGoogle Scholar
  4. Bartels, D., Altosaar, I., Harberd, N.P., Barker, R.F., Thompson, R.D. 1986. Molecular analysis of gamma-gliadin gene families at the complex Gli-1 locus of bread wheat (T. aestivum L.). Theor. Appl. Genet. 72: 845–853.CrossRefGoogle Scholar
  5. Cornish, G.B., Bekes, F., Allen, H.M., Martin, D.J. 2001. Flour proteins linked to quality traits in an Australian doubled haploid wheat population. Australian J. Agric. Res. 52: 1339–1348.CrossRefGoogle Scholar
  6. D’Ovidio, R., Tanzarella, O.A., Porceddu, E. 1991. Cloning and sequencing of a PCR amplified gamma-gliadin gene from durum wheat (Triticum turgidum (L.) Thell. conv. Durum (Desf.) MK.). Plant Sci. 75: 229–236.CrossRefGoogle Scholar
  7. Fido, R.J., Bekes, F., Gras, P.W., Tatham, A.S. 1997. Effects of α -, β -, γ and ω -gliadins on the dough mixing properties of wheat flour. J. Cereal Sci. 26: 271–277.CrossRefGoogle Scholar
  8. Huang, S.X., Sirikhachornkit, A., Su, X.J., Faris, J., Gill, B., Haselkorn, R., Gornicki, P. 2002. Genes encoding plastid acetyl-CoA carboxylase and 3-phosphoglycerate kinase of the Triticum-Aegilops complex and the evolutionary history of polyploid wheat. Proc. Natl. Acad. Sci. USA 99: 8133–8138.CrossRefGoogle Scholar
  9. Kerby, K., Kuspira, J. 1988. Cytological evidence bearing on the origin of the B genome in polyploid wheats. Genome 30: 36–43.CrossRefGoogle Scholar
  10. Khatkar, B.S., Fido, R.J., Tat Anderson, O.D., Greene, F.C. 1989. The characterization and comparative analysis of High-Molecular-Weight glutenin genes from genomes A and B of a hexaploid bread wheat. Theor. Appl. Genet. 77: 689–700.CrossRefGoogle Scholar
  11. Khatkar, B.S., Fido, R.J., Tatham, A.S., Schofield, J.D. 2002. Functional properties of wheat gliadins. I. Effects on mixing characteristics and bread making quality. J. Cereal Sci. 35: 299–306.CrossRefGoogle Scholar
  12. Kumar, S., Tamura, K., Nei, M. 2004. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief. Bioinform 5: 150–163.CrossRefGoogle Scholar
  13. Long, H., Wei, Y.-M., Yan, Z.-H., Baum, B., Nevo, E., Zheng, Y.L. 2005. Classification of wheat low-molecular-weight glutenin subunit genes and its chromosome assignment by developing LMW-GS group-specific primers. Theor. Appl. Genet. 111: 1251–1259.CrossRefGoogle Scholar
  14. MacRitchie, F. 1992. Physicochemical properties of wheat proteins in relation to functionality. Advanced Food Nutrition Res. 36: 1–87.CrossRefGoogle Scholar
  15. Maruyama, N., Ichise, K., Katsube, T., Kishimoto, T., Kawase, S., Matsumura, Y., Takeuchi, Y., Sawada, T., Utsumi, S. 1998. Identification of major wheat allergens by means of the Escherichia coli expression system. Eur. J. Biochem. 255: 739–745.CrossRefGoogle Scholar
  16. Masci, S., Lew, E.J-L., Lafiandra, D., Porceddu, E., Kasarda, D.D. 1995. Characterization of low-molecular-weight glutenin subunits in durum wheat by reversed-phase high-performance liquid chromatography and N-terminal sequencing. Cereal Chem. 72: 100–104.Google Scholar
  17. Metakovskii, E.V., Wrigley, C.W., Bekes, F., Gupta, R.B. 1990. Gluten polypeptides as useful genetic markers of dough quality in Australian wheats. Australian J. Agric. Res. 41: 289–306.CrossRefGoogle Scholar
  18. Metakovsky, E.V., Novoselskaya, A.Y., Sozinov, A.A. 1984. Genetic analysis of gliadin components in winter wheat using two-dimensional polyacrylamide gel electrophoresis. Theor. Appl. Genet. 69: 31–37.CrossRefGoogle Scholar
  19. Müller, S., Wieser, H. 1997. The location of disulphide bonds in monomeric α -type gliadins. J. Cereal Sci. 26: 169–176.CrossRefGoogle Scholar
  20. Müller, S.W., Wieser, H. 1995. The location of disulphide determination of gliadin subgroups from different wheat bonds in α -type gliadins. J. Cereal Sci. 22: 21–27.CrossRefGoogle Scholar
  21. Murray, M., Thompson, W.F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8: 4321–4325CrossRefGoogle Scholar
  22. Patacchini, C. 2003. Structural characteristics of low molecular weight glutenin subunits and their influence in determining technological properties of durum wheat. Ph.D. Dissertation Thesis. University of Verona, Italy.Google Scholar
  23. Payne, P.I., Seekings, J.A., Worland, A.J., Jarvis, M.G., Holt, L.M. 1987. Allelic variation of glutenin subunits and gliadins and its effect on bread making quality in wheat: analysis of F5 progeny from Chinese Spring × Chinese Spring (Hope 1A). J. Cereal Sci. 6: 103–118.CrossRefGoogle Scholar
  24. Petersen, G., Seberg, O., Yde, M., Berthelsen, K. 2006. Phylogenetic relationships of Triticum and Aegilops and evidence for the origin of the A, B, and D genomes of common wheat (Triticum aestivum). Mol. Phylogenet. and Evol. 39: 70–82.CrossRefGoogle Scholar
  25. Pistón, F., Dorado, G., Martín, A., Barro, F. 2006. Cloning of nine γ -gliadin mRNAs (cDNAs) from wheat and the molecular characterization of comparative transcript levels of γ -gliadin subclasses. J. Cereal Sci. 43: 120–128.CrossRefGoogle Scholar
  26. Qi, P.F., Wei, Y.M., Ouellet, T., Chen, Q., Tan, X., Zheng, Y.L. 2009. The γ -gliadin multigene family in common wheat (Triticum aestivum) and its closely related species. BMC Genomics 10: 168.CrossRefGoogle Scholar
  27. Rafalski, J.A. 1986. Structure of wheat gamma-gliadin genes. Gene 43: 221–229.CrossRefGoogle Scholar
  28. Scheets, K., Hedgcoth, C. 1988. Nucleotide sequence of a gamma gliadin gene: Comparisons with other gamma gliadin sequences show the structure of gamma gliadin genes and the general primary structure of gamma gliadins. Plant Sci. 57: 141–150.CrossRefGoogle Scholar
  29. Shewry, P.R., Halford, N.G., Tatham, A.S. 1989. The high molecular weight subunits of wheat, barley and rye: genetics, molecular biology, chemistry and role in wheat gluten structure and functionality. Oxford Surv. Plant Mol. Cell Biol. 6: 163–219.Google Scholar
  30. Shewry, P.R., Halford, N.G., Tatham, A.S. 1992. The high molecular weight subunits of wheat glutenin. J. Cereal Sci. 15: 105–120.CrossRefGoogle Scholar
  31. Shewry, P.R., Tatham, A.S., Lazzeri, P. 1997. Biotechnology of wheat quality. J. Sci. Food Agric. 73: 397–406.CrossRefGoogle Scholar
  32. Singh, N.K., Shepherd, K.W. 1988. Linkage mapping of genes controlling endosperm storage proteins in wheat. 1. Genes on the short arms of group-1 chromosomes. Theor. Appl. Genet. 75: 628–641.CrossRefGoogle Scholar
  33. Uthayakumaran, S., Tömösközi, S., Tatham, A.S., Savage, A.W.J., Gianibelli, M.C., Stoddard, F.L., Bekes, F. 2001. Effects of gliadin fractions on functional properties of wheat dough depending on molecular size and hydrophobicity. Cereal Chem. 78: 138–141.CrossRefGoogle Scholar
  34. von Büren, M. 2001. Polymorphisms in two homeologous γ -gliadin genes and the evolution of cultivated wheat. Gene Resour. Crop Evol. 48: 205–220.CrossRefGoogle Scholar
  35. Wang, J.R., Yan, Z.H., Jiang, Q.T., Wei, Y.M., Baum, B., Zheng, Y.-L. 2007. Sequence variations and molecular phylogenetic analyses of the HMW-GS genes from different genomes in Triticeae. Biochem. Syst. Ecol. 35: 421–433.CrossRefGoogle Scholar
  36. Zhang, W., Gianibelli, M.C., Ma, W., Rampling, L., Gale, K.R. 2003. Identification of SNPs and development of allele-specific PCR markers for γ -gliadin alleles in Triticum aestivum. Theor. Appl. Genet. 107: 130–138.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2010

Authors and Affiliations

  1. 1.Dujiangyan Campus, Sichuan Agricultural UniversityDujiangyan, SichuanChina
  2. 2.Chengdu Institute of Biology ChengduChinese Academy of SciencesSichuanChina
  3. 3.Triticeae Research InstituteSichuan Agricultural UniversityYaan, SichuanChina
  4. 4.Key Laboratory of Southwestern Crop Germplasm Utilization, Ministry of AgricultureThe People’s Republic of ChinaYaan, SichuanChina

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