Characterization and classification of γ-gliadin multigene sequences from Aegilops section Sitopsis

Abstract

The most abundant seed storage proteins of wheat are gliadins and glutenins. Gliadins, including α/β, γ and ω types, are normally monomeric proteins and account for about 50% of the gluten proteins. In this study, 55 sequences of γ-gliadin genes were obtained from species of Sitopsis section, the deduced B genome donors of wheat. Despite the high sequence similarities to the known γ-gliadin genes, extensive variations were also found. Using the extensive sequence information deposited in database and obtained in this study, a comprehensive classification of the γ-gliadin multigene families were performed based on the primary structures and phylogenic analysis. All the γ-gliadin genes analyzed could be divided into 2 types, which contain 8 and 9 cysteines, respectively. Type I (with 8 cysteines) and type II (with 9 cysteines) are further classified to 7 and 4 groups, respectively, and several subgroups are also identified. The genes derived from A, B and D genomes of common wheat were clustered distinctly, indicating that there was apparent genomic specificity in γ-gliadins genes. Besides the high homology between γ-gliadin genes from Sitopsis species and B genome of wheat, some unique groups or subgroups were also identified in Sitopsis section, suggesting that it could be considered as a valuable source of γ-gliadin genes. The comparison of deduced primary structures of each group and/or subgroup was conducted, from which their evolution and quality properties were also speculated.

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Correspondence to Y. M. Wei or Y. L. Zheng.

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Communicated by F. Békés

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Huang, Z., Long, H., Wei, Y.M. et al. Characterization and classification of γ-gliadin multigene sequences from Aegilops section Sitopsis. CEREAL RESEARCH COMMUNICATIONS 38, 1–14 (2010). https://doi.org/10.1556/CRC.38.2010.1.1

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Keywords

  • wheat
  • Aegilops
  • seed storage protein
  • γ-gliadin