Advertisement

Cereal Research Communications

, Volume 37, Issue 4, pp 551–558 | Cite as

Allelic diversity in Bulgarian winter wheat varieties based on polymorphism of glutenin subunit composition

  • N. TsenovEmail author
  • D. Atanasova
  • I. Todorov
  • I. Ivanova
  • I. Stoeva
Quality and Utilization

Abstract

Seventy-three common winter wheat varieties developed throughout the breeding history of the DAI were investigated for their diversity of allelic variants on storage proteins component composition in grain. The high- and low molecular weight protein structures were determined by the widely used SDS-PAGE method of Payne et al. (1980). The ratio between the individual alleles on the loci of high and low molecular weight glutenin was analyzed. The change in the HMW-score was followed according to the period when the respective varieties were developed. The configuration Glu-A1b, GluB1c, Glu-D1d, which was established in about 45% of the investigated genotypes, was most frequent in the high-molecular variants of glutenin. Concerning the low-molecular weight glutenins in 21 out of the 73 investigated varieties, the combination Glu-A3c, Glu-B3b, Glu-D3c was observed; it coincided with the spectrum of the widely used variety Bezostaya 1. Highest diversity was established in the allelic variants of Glu-A3, Glu-B3, in which 6 and 5 alleles, respectively, were observed. The quality of the varieties developed at DAI was relatively high (score 8.1). About a quarter of them are with high end-use quality confirmed in production. This is mainly due to the concentration of “strong” positive alleles in glutenin as a result from the intensive use of parents directly or indirectly related to Bezostaya 1, which lead to decrease of the percent of Glu-B1a (2 + 12). Quality should be further increased by additional diversity of combinations between “possitive” as effect on end-use grain quality alleles. Concerning HMW loci to keep of high grain end-use quality, it is necessary to maintain the status of Glu-A1b (2*), as well as of Glu-D1d (5 + 10).

Keywords

wheat quality varieties high- and low-molecular subunits HMW-score 

Abbreviations

HMW

high molecular weight

GS

glutenin subunits

LMW

low molecular weight

DAI

Dobrudzha Agricultural Institute

SDS-PAGE

sodium dodecylsulphate polyacrylamide gel

HRWW

hard red winter wheat

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Atanasova, D., Tsenov, N., Todorov, I., Ivanova, I. 2009. Glutenin composition of winter wheat varieties bred in Dobrudzha agricultural institute. Bulgarian Journal of Agricultural Science 15: 9–19.Google Scholar
  2. Blanlard, G., Dardevet, M., Saccomano, R., Lagoutte, F., Gourdon, J. 2001. Genetic diversity of wheat storage proteins and bread wheat quality. Breeding Sci. 56:59–67.Google Scholar
  3. Bushuk, W. 1998. Wheat breeding for end-product use. Euphytica 100:137–145.CrossRefGoogle Scholar
  4. Denčič, S., Kobiljski, B. 2008. Results of half a century of wheat breeding at Institute of Field and Vegetable Crops in Novi Sad. Proceedings of International Conference “Conventional and Molecular Breeding of Field and Vegetable Crops”, 24–27 Nov 2008, Novi Sad, Serbia, pp. 377–382.Google Scholar
  5. Deng, Z.Y., Tian, J.C., Sun, G.X. 2005. Influence of high molecular weight glutenin subunit substitution on rheological behavior and bread-baking quality of near-isogenic lines developed from Chinese wheats. Plant Breeding 124:428–431.CrossRefGoogle Scholar
  6. Eagles, H.A., Cane, K., Eastwood, R.F., Hollamby, G.J., Kuchel, H., Martin, E.M., Cornish G.B. 2006. Contributions of glutenin and puroindoline genes to grain quality traits in southern Australian wheat breeding programs. Australian J. Agric. Res. 57:179–186.CrossRefGoogle Scholar
  7. Gobaa, S. 2007. Impact of prolamin variation and 1BL.1RS translocation on bread-making quality parameters of wheat (Triticum aestivum L.). Doctor of Sciences, Zurich University, 86 pp.Google Scholar
  8. Gras, P.W., Anderssen, R.S., Keentok, M., Békés, F., Appels, R. 2001. Gluten protein functionality in wheat flour processing: a review. Australian J. Agric. Res. 52:1311–1323.CrossRefGoogle Scholar
  9. Graybosch, R. 1992. High molecular weight glutenin subunit composition of cultivars, germplasm, and parents of U.S. red winter wheat. Crop Sci. 32:1151–1155.CrossRefGoogle Scholar
  10. Gregova, E., Mihalik, D., Slikova, S., Sramkova, Z. 2007. Allelic variation of HMW glutenin subunits and 1BL.1RS Translocation in Slovak common wheats. Cereal Res. Commun. 35:1675–1683.CrossRefGoogle Scholar
  11. Gupta, R.B., Shepherd, K.W. 1990. Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutenin. 1. Variation and genetic control of the subunits in hexaploid wheats. Theor. Appl. Gen. 80:65–74.CrossRefGoogle Scholar
  12. Ivanov, P., Todorov, I., Ivanova, I. 2000. The high molecular weight glutenin subunit composition of Bulgarian bread wheat (Triticum aestivum L.) varieties bred at the Dobroudja Agricultural Institute. Genetics and Breeding 30:3–9.Google Scholar
  13. Jackson, E.A., Morel, M.H., Sontag-Strohm, T., Branlard, G., Metakovsky, E.V., Redaelli, R. 1996. Proposal for combining the classification systems of alleles of Gli-1 and Glu-3 loci in bread wheat (Triticum aestivum L.). J. Genet. and Breeding 50:321–336.Google Scholar
  14. Liu, Y., Xiong, Z.Y., He, Y.G., Shewry, P.R., He, G.Y. 2007. Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province. Genetic Resources and Crop Evolution 54:865–874.CrossRefGoogle Scholar
  15. Morgunov, A.I., Pena, R.J., Crossa, J., Rajaram, S. 1993. Worldwide distribution of Glu-1 alleles in bread wheat. J. Genet. and Breeding 47:53–60.Google Scholar
  16. Obreht, D., Kobiljski, B., Dan, M., Vapa, L. 2008. Marker assisted selection in BMQ related breeding programs. Genetika (Serbia) 40:39–49.CrossRefGoogle Scholar
  17. Panayotov, I. Todorov, I., Tsenov, N., Stoeva, I. 1992. Main results of wheat breeding in Bulgaria. Paper of Agric. Sci. 7:62–68 (in Russian)Google Scholar
  18. Payne, P.I., Lawrence, G.J. 1983. Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for hogh-molecular-weight subunit in hexaploid wheat. Cereal Res. Commun. 11:29–35.Google Scholar
  19. Payne, P.I., Law, C.N., Mudd, E.E. 1980. Control by homoeologous group 1 chromosomes of the high-molecular weight subunits of glutenin, a major protein of wheat endosperm. Theor. Appl. Genet. 58:113–120.CrossRefGoogle Scholar
  20. Payne, P.I., Nightingale, M.A., Krattiger, A.F., Holt, L.M. 1987. The relationship between HMW glutenin subunit composition and the bread making quality of British-grown wheat varieties. J. Sci. and Food Agricult. 40:51–65.CrossRefGoogle Scholar
  21. Shewry, P.R, Tatham, A.S., Fido, R., Jones, H., Barcelo, P., Lazzeri, P.A. 2001. Improving the end use properties of wheat by manipulating the grain protein composition. Euphytica 119:45–48.CrossRefGoogle Scholar
  22. Singh, N.K., Shepherd, K.W., Cornish, G.B. 1991. Asimplified SDS-PAGE procedure for separating LMW subunits of glutenin. J. Cereal Sci. 14:203–280.CrossRefGoogle Scholar
  23. Sultana, T., Ghafoor, A., Ashraf, M. 2007. Genetic variability in bread wheat (Triticum aestivum L.) of Pakistan based on polymorphism for high molecular weight glutenin subunits. Genet. Resources and Crop Evol. 54:1159–1165.CrossRefGoogle Scholar
  24. Takibi, T., Ikeguchi, S., Ikeda, T.M. 2006. Effects of high-molecular-weight and low-molecular-weight glutenin subunits alleles on common wheat flour quality. Breeding Sci. 56:131–136.CrossRefGoogle Scholar
  25. Tanaka, H., Shimizu, R., Tsujimoto, H. 2005. Genetical analysis of contribution of low-molecular-weight glutenin subunits to dough strength in common wheat (Triticum aestivum L.). Euphytica 141:157–162.CrossRefGoogle Scholar
  26. Todorov, I. 2006. Investigation of grain storage proteins and their use as markers in wheat breeding, Thesis for Dr. of Science, DAI, General Toshevo, 398 pp. (In Bulgarian)Google Scholar
  27. Todorov, I., Ivanov, P., Ivanova, I. 2006. Genetic diversity of high molecular weight glutenin allels in varieties and lines with different origin, Field Crops Studies 3:487–499. (In Bulgarian)Google Scholar
  28. Todorov, I., Ivanov, P., Ivanova, I., Penchev, E. 2004. Investigation on genetic diversity of Bulgarian varieties (T. aestivum L.) using biochemical markers. Field Crops Studies 1:34–42 (In Bulgarian)Google Scholar
  29. Tohver, M. 2007. High molecular weight (HMW) glutenin subunit composition of Nordic and Middle European wheats. Genet. Resources and Crop Evol. 54:67–81.CrossRefGoogle Scholar
  30. Trethowan, R.M., Pena, R.J., van Ginkel, M. 2001. Breeding for grain quality: A manipulation of gene frequency. In: Bedö, Z., Lang, L. (eds): Wheat in a Global Environment — Proc. 6th Int. Wheat Conf., Kluwert Acad. Publ., Dordrecht, The Netherlands, pp. 263–271.CrossRefGoogle Scholar
  31. Xu, Q., Xu, J., Liu, C.L., Chang, C., Wang, C.P., You, M.S., Li, B.Y., Liu, G.T. 2008. PCR-based markers for identification of HMW-GS at Glu-B1x loci in common wheat. J. Cereal Sci. 47:394–398.CrossRefGoogle Scholar
  32. Zhang, X. K., Liu, L.Z.H., He, Z.H., Sun, D.J., He, X.Y., Xu, Z.H., Zhang, P.P., Chen, F., Xia, X.C. 2008. Development of two multiplex PCR assays targeting improvement of bread-making and noodle qualities in common wheat. Plant Breeding 127:109–115.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2009

Authors and Affiliations

  • N. Tsenov
    • 1
    Email author
  • D. Atanasova
    • 1
  • I. Todorov
    • 1
  • I. Ivanova
    • 1
  • I. Stoeva
    • 1
  1. 1.Dobrudzha Agricultural InstituteGeneral ToshevoBulgaria

Personalised recommendations