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Cereal Research Communications

, Volume 37, Issue 3, pp 363–372 | Cite as

Genomic distribution of a long terminal repeat (LTR) Sabrina-like retrotransposon in Triticeae species

  • C. Liu
  • Z. J. YangEmail author
  • J. Q. Jia
  • G. R. Li
  • J. P. Zhou
  • Z. L. RenEmail author
Genetics

Abstract

Knowledge of the chromosomal distribution of long terminal repeats (LTR) is important for understanding plant chromosome structure, genomic organization and evolution, as well as providing chromosomal landmarks that are useful for chromosome engineering. The aim of this study is to investigate the genomic distribution of Sabrina-like LTR pDbH12, which was first isolated from Dasypyrum breviaristatum (Vb genome), on Triticeae species in relation to the genomic evolution and chromosome identification. Fluorescence in situ hybridization (FISH) analysis showed that pDbH12 is present on Dasypyrum (V genome) and Hordeum (H genome) species with the hybridized signals covering the entire chromosomes. However, clone pDbH12 did not hybridize to the genomes of Secale, Triticum, Lophopyrum, Pseduoroengeria, Aegilops, Agropyron desertorum and Elymus. Thinopyrum intermedium displayed fourteen chromosomes that hybridized with pDbH12. Sequential FISH identified these chromosomes as belonging to the Js genome. Results from sequence characterized amplified region (SCAR) marker and dot blot both support the FISH results, and the integrative results suggest that amplification of Sabrina-like LTR retrotransposons is an important factor which involved in the speciation process. Clone pDbH12 could serve as a cytogenetic marker for tracing chromatin from V or Vb, H and Js genomes in wheat-alien introgression lines.

Keywords

Sabrina-like LTR Dasypyrum Thinopyrum dot blot in situ hybridization 

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Copyright information

© Akadémiai Kiadó, Budapest 2009

Authors and Affiliations

  1. 1.School of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengduP. R. China
  2. 2.Key Laboratory for Plant Genetics and BreedingSichuan Agricultural UniversityYa’an CityP. R. China

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