Cereal Research Communications

, Volume 37, Issue 3, pp 409–417 | Cite as

Cell membrane stability: Combining ability and gene effects under heat stress conditions

  • S. S. Dhanda
  • R. MunjalEmail author


Heat stress is an important production constraint of wheat during grain-fill period in India and in other parts of the world where the temperature become high during anthesis to maturity (grain-filling) stage of plant growth. This study determined the genetic control of heat tolerance through half diallel analysis of selected wheat genotypes. Heat induced damage of plasma membrane was assayed by membrane thermo-stability (MTS), which measure electrolyte leakage from leaf tissues after exposure to high temperature. Eight genotypes comprising heat tolerant and sensitive response to high temperature stress were hybridized in a half diallel. Electrolyte leakage or MTS was conducted at grain-filling stage of plant growth as ambient temperature become high enough to cause heat hardening of leaves. The mean square for GCA was higher in magnitude than that of SCA, but the components of genetic variance indicated considerable influence of dominance variance in determining inheritance of this trait. Results suggested that the selection for heat tolerant inbred lines based on MTS in this material may be more effective by reducing the dominance variance after a few generation of selfing particularly in a self-pollinated wheat crop. The varieties, Hindi 62 and NIAW 34 were good general and specific combiners in the tolerant group, while HD 2687 and WH 147 were good specific combiners in the heat sensitive group.


cell membrane stability heat stress combining ability gene effects heat susceptibility index heat response index 


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  1. Bjorkman, O., Badger, M.R., Armond, P.A. 1980. Response and adaptation of photosynthesis to high temperature. In: Turner, N.C., Cramer, P.J. (eds), Adaptation of Plants to Water and High Temperature Stress. John Wiely & Sons, New York, pp. 233–239.Google Scholar
  2. Blum, A. 1988. Plant Breeding for Stress Environments. CRC Press, Inc., Boca Raton. pp. 201–220.Google Scholar
  3. Blum, A., Ebercon, A. 1981. Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Sci. 21:43–47.CrossRefGoogle Scholar
  4. Chen, T.H.H., Shen, Z.Y., Li, P.H. 1982. Adaptability of crop plants to high temperature stress. Crop Sci. 22:719–725.CrossRefGoogle Scholar
  5. Dhanda, S.S., Munjal, R. 2006. Inheritance of cellular thermotolerance in bread wheat. Plant Breeding (Germany) 125:557–567.CrossRefGoogle Scholar
  6. Evans, L.T. 1978. The inheritance of irradiance before and after anthesis on grain yield and its components in micro plots of wheat grown in constant day length and temperature regime. Field Crop Res. 1:5–19.CrossRefGoogle Scholar
  7. Fischer, R.A. 2007. Understanding the physiological basis of yield potential in wheat. J. Agric. Sci. (Cambridge) 145:99–113.CrossRefGoogle Scholar
  8. Fischer, R.A., Byerlee, D.B. 1991. Trends of wheat production in the warmer areas: major issues and economic considerations. In: Wheat for the Non-traditional Warm Areas. Proc. of Conf., Iguazu, Brazil, 29 Jul.–3 Aug. 1990, Mexico, DF, CIMMYT, pp. 3–27.Google Scholar
  9. Fokar, M., Henry, T.N., Blum, A. 1998. Heat tolerance in spring wheat I. Estimating cellular thermo-tolerance and its heritability. Euphytica 104:1–8.CrossRefGoogle Scholar
  10. Griffing, B. 1956. The concept of general and specific combining ability in relation to diallel crossing systems. Aust. J. Biol. Sci. 9:463–493.CrossRefGoogle Scholar
  11. Hawker, J.S., Jenner, C.F. 1993. High temperature affects the activity of enzymes in the committed pathway of starch synthesis in developing wheat endosperm. Aust. J. Plant Physiol. 20:197–209.Google Scholar
  12. Ibrahim, A.M.H., Quick, J. 2001. Genetic control of high temperature in wheat as measured by membrane thermal stability. Crop Sci. 41:1405–1407.CrossRefGoogle Scholar
  13. Krishnan, M., Nguyen, H.T., Burke, J.J. 1989. Heat shock protein synthesis and thermal tolerance in wheat. Plant Physiol. 90:140–145.CrossRefGoogle Scholar
  14. Lin, C.Y., Chen, Y.M., Key, J.L. 1985. Solute leakage in soybean seedlings under various heat shock regimes. Plant Cell Physiol. 26:1493–1498.Google Scholar
  15. Nagrajan, S. 2005. Can India produce enough wheat even by 2020. Curr. Sci. 89:1467–1471.Google Scholar
  16. Porter, D.R., Nguyen, H.T. Burke, J.J. 1995. Genetic control of acquired high temperature tolerance in winter wheat. Euphytica 83:153–157.CrossRefGoogle Scholar
  17. Raison, J. K., Berry, P.A., Armond, C., Pike, S. 1980. Membrane properties in relation to the adaptation of plants to temperature stress. In: Turner, N.C., Kramer, P.J. (eds), Adaptation of Plants to Water and High Temperature Stress. John Wiely & Sons, New York, pp. 261–273.Google Scholar
  18. Rane Jagadish, Pannu, R.K., Sohu, V.S., Saini, R.S., Mishra, B, Shoran Jag, Crossa, J., Vargas, M., Joshi, A.K. 2007. Performance of yield and stability of advanced wheat genotypes under heat stress environments of the Indo-Gangetic Plains. Crop Sci. 47:1561–1573.CrossRefGoogle Scholar
  19. Reynolds, M.P., Balota, M., Delgado, M.I.B., Amani, I., Fisher, R.A. 1994. Physiological and morphological traits associated with spring wheat under hot irrigated conditions. Aust. J. Plant Physiol. 21:717–730.Google Scholar
  20. Reynolds, M.P., Trethowan, R., Crossa, J., Vargas, M., Sayre, K.D. 2002. Physiological factors associated with genotype by environment interaction in wheat. Field Crops Res. 75:139–160.CrossRefGoogle Scholar
  21. Richards, R.A., Watt, M., Rebetzke, G.J. 2007. Physiological traits and cereal germplasm for sustainable agricultural systems. Euphytica 154:409–425.CrossRefGoogle Scholar
  22. Saadalla, M.M. 1997. Inheritance of cell membrane thermo-stability as a criterion for heat tolerance in wheat. Alexandria J. Agric. Res. 42:15–26.Google Scholar
  23. Sullivan, Y.C. 1972. Mechanisms of heat and drought tolerance in grain sorghum and methods of measurements. In: Rao, N.G.P., House, L.R. (eds), Sorghum in Seventies. Oxford and IBH, New Delhi, pp. 247–264.Google Scholar
  24. Sullivan, C.Y., Ross, W.M. 1979. Selection for drought and heat tolerance in grain sorghum. In: Mussel, H., Staples, R.C. (eds), Stress Physiology in Crop Plants. John Wiley & Sons, New York, pp. 263–281.Google Scholar
  25. Wardlaw, I.F., Dawson, I.A., Munibi, P., Fewster, R. 1989. The tolerance of wheat to high temperature during reproductive growth: Survey procedure and general response pattern. Aus. J. Agric. Res. 40:1–13.CrossRefGoogle Scholar
  26. Whitlow, T.H., Bassu, N.L., Ranney, T.G., Reichest, D.L. 1992. An improved method for using electrolyte leakage to assess competence in plant tissues. Plant Physiol. 98:98–205.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2009

Authors and Affiliations

  1. 1.Department of Plant BreedingCCS HAUHisarIndia

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