Distribution of Vp-1 alleles in Chinese white-grained landraces, historical and current wheat cultivars
Genotypes with various Vp-1B alleles perform different levels of pre-harvest sprouting (PHS) tolerance. In this study, 217 white-grained wheat cultivars, including 75 landraces, 39 historical cultivars, and 103 modern cultivars from five major regions of China, were examined to characterize the diversity of the Viviparous-1B (Vp-1B) locus associated with PHS tolerance. Four Vp-1B alleles were identified, three (Vp-1Ba, Vp-1Bb and Vp-1Bc) of which were previously reported in Chinese wheat cultivars. A new allele, Vp-1Be, was identified in the PHS tolerant landrace Hongheshangtou. Sequence analysis showed that Vp-1Be had an insertion of a 4-bp fragment, two SNPs, and a deletion of an 83-bp fragment compared with the nucleotide sequence of Vp-1Ba (AJ400713), all located in the third intron. Vp-1Be shared 97.80% similarity with the nucleotide sequence of AJ400713. The frequencies of Vp-1Ba, Vp-1Bb, and Vp-1Bc were 36.0%, 5.3%, and 57.3% in landraces; 23.1%, 7.7%, and 69.2% in historical cultivars; and 52.4%, 0%, and 47.6% in current cultivars, respectively.
KeywordsTriticum aestivum Vp-1 alleles pre-harvest sprouting seed dormancy
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