Cereal Research Communications

, Volume 37, Issue 2, pp 287–293 | Cite as

Estimated economic benefit of double-haploid technique for peruvian barley growers and breeders

  • L. R. Gomez-PandoEmail author
  • J. Jimenez-Davalos
  • A. Eguiluz-de la Barra
  • E. Aguilar-Castellanos
  • J. Falconí-Palomino
  • M. Ibañez-Tremolada
  • M. E. Aspiolea
  • J. C. Lorenzo


Although barley is extensively grown in the Peruvian highlands as a food crop, agronomic and quality performance are poor. In addition, there are few reports of the successful application of modern technologies, such as doubled haploidy, in developing nations. Double-haploid technique was used to obtain barley cultivars better suited to this environment. From barley crosses Ya/LM94, B16/LM94 and B12/LM94, four promising double-haploid lines were evaluated in the highland Acobamba Farming Community along with their parents and two control cultivars (PPch, UNA80). Double-haploid lines, Ya/LM94-PC27 and B12/LM94-PC34, were the most agronomically acceptable. Here we describe their potential economic benefits for Acobamba farmers. Both reached the best quality grade based on hectoliter mass, and should therefore obtain the highest market price, and provide the best incomes, gains, and profitability rates. In comparison, PPch and UNA80 if grown would be unprofitable, and LM94 would be markedly less profitable. Use of the double-haploid technique in our breeding program was estimated to cut research expenses by 26%, due to the shorter time for cultivar improvement.


Hordeum vulgare L. breeding anther in vitro culture economic profitability 



Puca Poncho








Yanamuclo 87


Double haploid


Cereal Program


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bhatty, R.S. 1993. Non-malting uses of barley. In: McGregor, A.W., Bhatty, R.S. (eds), Barley: Chemistry and Technology. American Association of Cereal Chemists, St. Paul, Minnesota, pp. 355–417.Google Scholar
  2. Cai, Q., Szarejko, I., Polok, K., Maluszynski, M. 1992. The effect of sugars and growth regulators on embryoid formation and plant regeneration from barley anther culture. Plant Breed. 109:218–226.CrossRefGoogle Scholar
  3. Chrispeels, M., Sadava, D. 2003. Development, productivity and sustainability of crop production. In: Chrispeels, M.J., Sadava, D. (eds), Plants, Genes and Crop Biotechnology. Jones and Bartlett Publishers, Inc, Sudbury, MA, Boston, Toronto, London, Singapore, pp. 52–75.Google Scholar
  4. FAOSTAT, 2008. Available via Food Agricultural Organization. Accessed 18 Apr 2008
  5. IPGRI, 1994. Descriptors for barley (Hordeum vulgare L.). International Plant Genetic Resources Institute, Rome, pp. 1–46.Google Scholar
  6. Jensen, N. 1988. Breeding and Selection Methods: Double Haploid Method. Plant Breeding Methodology. John Wiley and Sons, New York, pp. 15–62, 297–302.Google Scholar
  7. Kasha, K., Hu, T., Oro, R., Simion, E., Shim, Y. 2001a. Nuclear fusion leads to chromosome doubling during mannitol pretreatment of barley (Hordeum vulgare L.) microspores. J. Exp. Bot. 52:1227–1238.PubMedGoogle Scholar
  8. Kasha, K., Simion, E., Oro, R., Yao, Q., Hu, T., Carlson, A. 2001b. An improved in vitro technique for isolated microspore culture of barley. Euphytica 120:379–385.CrossRefGoogle Scholar
  9. Maluszynski, M., Kasha, K., Szarejko, I. 2003. Published doubled haploid protocols in plant species. In: Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. (eds), Doubled Haploid Production in Crop Plants: A Manual. Kluwer Academic Publishers, Dordrecht, Boston, London, pp. 309–335.CrossRefGoogle Scholar
  10. MINAG 2007. Available via Peruvian Ministry of Agriculture. Accessed 7 Mar 2007
  11. Ortiz, R., Nurminiemi, M., Madsen, S., Rognli, O., Bjørnstad, A. 2002. Genetic gains in Nordic spring barley breeding over sixty years. Euphytica 126:283–289.CrossRefGoogle Scholar
  12. Palmer, C., Keller, W. 2005. Overview of haploidy: Haploids in crop improvement II. Biotech. Agric. Forest 56:1–7.Google Scholar
  13. Roelfs, A., Singh, R., Saari, E. 1992. Wheat rusts: Concepts and methods to manage them. CIMMYT, Ciudad México, pp. 1–81.Google Scholar
  14. Szarejko, I., Kasha, K. 1991. Induction of anther culture derived doubled haploids in barley. Cereal Res. Comm. 19:219–237.Google Scholar
  15. Thomas, W. 2002. Molecular marker-assisted versus conventional selection in barley breeding. In: Slafer, G.A., Molina-Cano, J., Savin, R., Araus, J., Romagosa, I. (eds), Barley Science: Recent Advances from Molecular Biology to Agronomy, New Views on the Origin of Cultivated Barley. The Haworth Press, Inc., New York, pp. 177–204.Google Scholar
  16. Thomas, W., Forster, B., Gertsson, B. 2003. Doubled haploids in breeding. In: Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. (eds), Doubled Haploid Production in Crop Plants: A Manual. Kluwer Academic Publishers, Dordrecht, Boston, London, pp. 337–349.CrossRefGoogle Scholar
  17. Toojinda, T., Broers, L.H., Chen, X.M., Hayes, P.M., Kleinhofs, A., Korte, J., Kudrna, D., Leung, H., Line, R.F., Powell, W., Ramsay, L., Vivar, H., Waugh, R. 2000. Mapping quantitative and qualitative disease resistance genes in doubled haploid population barley (Hordeum vulgare). Theor. Appl. Genet. 101:580–589.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2009

Authors and Affiliations

  • L. R. Gomez-Pando
    • 1
    Email author
  • J. Jimenez-Davalos
    • 1
  • A. Eguiluz-de la Barra
    • 1
  • E. Aguilar-Castellanos
    • 1
  • J. Falconí-Palomino
    • 1
  • M. Ibañez-Tremolada
    • 1
  • M. E. Aspiolea
    • 2
  • J. C. Lorenzo
    • 3
  1. 1.Cereal Research ProgramNational Agricultural University La MolinaLimaPeru
  2. 2.Faculty of Economical SciencesUniversity of Ciego de AvilaCiego de AvilaCuba
  3. 3.Laboratory for Plant Breeding, Bioplant CentreUniversity of Ciego de AvilaCiego de AvilaCuba

Personalised recommendations