Estimated economic benefit of double-haploid technique for peruvian barley growers and breeders

Abstract

Although barley is extensively grown in the Peruvian highlands as a food crop, agronomic and quality performance are poor. In addition, there are few reports of the successful application of modern technologies, such as doubled haploidy, in developing nations. Double-haploid technique was used to obtain barley cultivars better suited to this environment. From barley crosses Ya/LM94, B16/LM94 and B12/LM94, four promising double-haploid lines were evaluated in the highland Acobamba Farming Community along with their parents and two control cultivars (PPch, UNA80). Double-haploid lines, Ya/LM94-PC27 and B12/LM94-PC34, were the most agronomically acceptable. Here we describe their potential economic benefits for Acobamba farmers. Both reached the best quality grade based on hectoliter mass, and should therefore obtain the highest market price, and provide the best incomes, gains, and profitability rates. In comparison, PPch and UNA80 if grown would be unprofitable, and LM94 would be markedly less profitable. Use of the double-haploid technique in our breeding program was estimated to cut research expenses by 26%, due to the shorter time for cultivar improvement.

Abbreviations

PPch:

Puca Poncho

LM94:

UNALM94

B12:

B-1205

B16:

B-1602

Ya:

Yanamuclo 87

DH:

Double haploid

PC:

Cereal Program

References

  1. Bhatty, R.S. 1993. Non-malting uses of barley. In: McGregor, A.W., Bhatty, R.S. (eds), Barley: Chemistry and Technology. American Association of Cereal Chemists, St. Paul, Minnesota, pp. 355–417.

    Google Scholar 

  2. Cai, Q., Szarejko, I., Polok, K., Maluszynski, M. 1992. The effect of sugars and growth regulators on embryoid formation and plant regeneration from barley anther culture. Plant Breed. 109:218–226.

    CAS  Article  Google Scholar 

  3. Chrispeels, M., Sadava, D. 2003. Development, productivity and sustainability of crop production. In: Chrispeels, M.J., Sadava, D. (eds), Plants, Genes and Crop Biotechnology. Jones and Bartlett Publishers, Inc, Sudbury, MA, Boston, Toronto, London, Singapore, pp. 52–75.

    Google Scholar 

  4. FAOSTAT, 2008. Available via Food Agricultural Organization. http://www.fao.org/docrep/008/a0050e00.htm. Accessed 18 Apr 2008

  5. IPGRI, 1994. Descriptors for barley (Hordeum vulgare L.). International Plant Genetic Resources Institute, Rome, pp. 1–46.

    Google Scholar 

  6. Jensen, N. 1988. Breeding and Selection Methods: Double Haploid Method. Plant Breeding Methodology. John Wiley and Sons, New York, pp. 15–62, 297–302.

    Google Scholar 

  7. Kasha, K., Hu, T., Oro, R., Simion, E., Shim, Y. 2001a. Nuclear fusion leads to chromosome doubling during mannitol pretreatment of barley (Hordeum vulgare L.) microspores. J. Exp. Bot. 52:1227–1238.

    CAS  PubMed  Google Scholar 

  8. Kasha, K., Simion, E., Oro, R., Yao, Q., Hu, T., Carlson, A. 2001b. An improved in vitro technique for isolated microspore culture of barley. Euphytica 120:379–385.

    Article  Google Scholar 

  9. Maluszynski, M., Kasha, K., Szarejko, I. 2003. Published doubled haploid protocols in plant species. In: Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. (eds), Doubled Haploid Production in Crop Plants: A Manual. Kluwer Academic Publishers, Dordrecht, Boston, London, pp. 309–335.

    Google Scholar 

  10. MINAG 2007. Available via Peruvian Ministry of Agriculture. http://www.minag.org.pe. Accessed 7 Mar 2007

  11. Ortiz, R., Nurminiemi, M., Madsen, S., Rognli, O., Bjørnstad, A. 2002. Genetic gains in Nordic spring barley breeding over sixty years. Euphytica 126:283–289.

    Article  Google Scholar 

  12. Palmer, C., Keller, W. 2005. Overview of haploidy: Haploids in crop improvement II. Biotech. Agric. Forest 56:1–7.

    Google Scholar 

  13. Roelfs, A., Singh, R., Saari, E. 1992. Wheat rusts: Concepts and methods to manage them. CIMMYT, Ciudad México, pp. 1–81.

    Google Scholar 

  14. Szarejko, I., Kasha, K. 1991. Induction of anther culture derived doubled haploids in barley. Cereal Res. Comm. 19:219–237.

    Google Scholar 

  15. Thomas, W. 2002. Molecular marker-assisted versus conventional selection in barley breeding. In: Slafer, G.A., Molina-Cano, J., Savin, R., Araus, J., Romagosa, I. (eds), Barley Science: Recent Advances from Molecular Biology to Agronomy, New Views on the Origin of Cultivated Barley. The Haworth Press, Inc., New York, pp. 177–204.

    Google Scholar 

  16. Thomas, W., Forster, B., Gertsson, B. 2003. Doubled haploids in breeding. In: Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. (eds), Doubled Haploid Production in Crop Plants: A Manual. Kluwer Academic Publishers, Dordrecht, Boston, London, pp. 337–349.

    Google Scholar 

  17. Toojinda, T., Broers, L.H., Chen, X.M., Hayes, P.M., Kleinhofs, A., Korte, J., Kudrna, D., Leung, H., Line, R.F., Powell, W., Ramsay, L., Vivar, H., Waugh, R. 2000. Mapping quantitative and qualitative disease resistance genes in doubled haploid population barley (Hordeum vulgare). Theor. Appl. Genet. 101:580–589.

    CAS  Article  Google Scholar 

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Correspondence to L. R. Gomez-Pando.

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Gomez-Pando, L.R., Jimenez-Davalos, J., Eguiluz-de la Barra, A. et al. Estimated economic benefit of double-haploid technique for peruvian barley growers and breeders. CEREAL RESEARCH COMMUNICATIONS 37, 287–293 (2009). https://doi.org/10.1556/CRC.37.2009.2.17

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Keywords

  • Hordeum vulgare L.
  • breeding
  • anther in vitro culture
  • economic profitability