Transposon-derived organ-specific mutation in the ADH1 RNA 5′ untranslated leader of autotetraploid maize (Zea mays L.)
A new unstable organ-specific Adh1 mutant was isolated from autotetraploid S 25 Wf9 maize line after germinating kernels under water for two days. Adh1-Fm4x-1 expresses normal and/or reduced levels of ADH1 in anaerobic scutellum. No activity or approximately 33–75% of wild type level of ADH1 in pollen grains was observed. The organ-specific phenotype of the Adh1-Fm4x-1 could be maintained by selfing for ten subsequent generations. After crossing with the wild-type allele the Adh1 mutation transmitted to the next generation both by female and male gametophytes. The appearance of one- and two-banded patterns in anaerobic scutellum and pollen grains of heterozygous F 1 plants showed the lack of F·F homodimers.
DNA sequence analyses of the wild type allele, the Adh1-Fm4x-1 mutant allele and four F 1 revertants revealed that the Adh1-Fm4x-1 mutation contains a Dissociation (Ds1) transposable element in the 5′ untranslated leader of the maize Adh1 gene, which regulates organ-specific expression at a quantitative level.
KeywordsDs1 element Adh1 autotetraploid maize unstable organ-specific mutation 5′ UTR point mutation
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- Chen, C.-H., Freeling, M., Mercklebach, A. 1986. Enzymatic and morphological consequences of Ds excisions from maize Adh1. Maydica (McClintock issue) 31:93–108.Google Scholar
- Hajósné, Novák M., H. Nagy, A. 1995. Az alkohol dehidrogenáz-1 (Adh1) gén kifejeződése tetraploid kukorica (Zea mays L.) pollenjében. [Expression of alcohol-dehydrogenase-1 (Adh1) gene in the pollen grains of autotetraploid maize (Zea mays L.)]. Növénytermelés 44:229–235.Google Scholar
- Williams, C.E., Kloeckener-Guissem, B., Freeling, M. 1991. Naturally occurring variants of maize Adh1 differ in organ-specific expression both in quantity and developmental timing. Maydica 36:115–128.Google Scholar