Transposon-derived organ-specific mutation in the ADH1 RNA 5′ untranslated leader of autotetraploid maize (Zea mays L.)

Abstract

A new unstable organ-specific Adh1 mutant was isolated from autotetraploid S 25 Wf9 maize line after germinating kernels under water for two days. Adh1-Fm4x-1 expresses normal and/or reduced levels of ADH1 in anaerobic scutellum. No activity or approximately 33–75% of wild type level of ADH1 in pollen grains was observed. The organ-specific phenotype of the Adh1-Fm4x-1 could be maintained by selfing for ten subsequent generations. After crossing with the wild-type allele the Adh1 mutation transmitted to the next generation both by female and male gametophytes. The appearance of one- and two-banded patterns in anaerobic scutellum and pollen grains of heterozygous F 1 plants showed the lack of F·F homodimers.

DNA sequence analyses of the wild type allele, the Adh1-Fm4x-1 mutant allele and four F 1 revertants revealed that the Adh1-Fm4x-1 mutation contains a Dissociation (Ds1) transposable element in the 5′ untranslated leader of the maize Adh1 gene, which regulates organ-specific expression at a quantitative level.

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Correspondence to M. Hajós-Novák.

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Hajós-Novák, M., Dallmann, G. & Kristóf, Z. Transposon-derived organ-specific mutation in the ADH1 RNA 5′ untranslated leader of autotetraploid maize (Zea mays L.). CEREAL RESEARCH COMMUNICATIONS 37, 151–158 (2009). https://doi.org/10.1556/CRC.37.2009.2.1

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Keywords

  • Ds1 element
  • Adh1
  • autotetraploid maize
  • unstable organ-specific mutation
  • 5′ UTR
  • point mutation