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Cereal Research Communications

, Volume 37, Issue 2, pp 151–158 | Cite as

Transposon-derived organ-specific mutation in the ADH1 RNA 5′ untranslated leader of autotetraploid maize (Zea mays L.)

  • M. Hajós-NovákEmail author
  • G. Dallmann
  • Z. Kristóf
Genetics

Abstract

A new unstable organ-specific Adh1 mutant was isolated from autotetraploid S 25 Wf9 maize line after germinating kernels under water for two days. Adh1-Fm4x-1 expresses normal and/or reduced levels of ADH1 in anaerobic scutellum. No activity or approximately 33–75% of wild type level of ADH1 in pollen grains was observed. The organ-specific phenotype of the Adh1-Fm4x-1 could be maintained by selfing for ten subsequent generations. After crossing with the wild-type allele the Adh1 mutation transmitted to the next generation both by female and male gametophytes. The appearance of one- and two-banded patterns in anaerobic scutellum and pollen grains of heterozygous F 1 plants showed the lack of F·F homodimers.

DNA sequence analyses of the wild type allele, the Adh1-Fm4x-1 mutant allele and four F 1 revertants revealed that the Adh1-Fm4x-1 mutation contains a Dissociation (Ds1) transposable element in the 5′ untranslated leader of the maize Adh1 gene, which regulates organ-specific expression at a quantitative level.

Keywords

Ds1 element Adh1 autotetraploid maize unstable organ-specific mutation 5′ UTR point mutation 

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Copyright information

© Akadémiai Kiadó, Budapest 2009

Authors and Affiliations

  1. 1.Institute for Genetics and Biotechnology, Section of Plant BreedingSzent István University (SZIE)GödöllőHungary
  2. 2.Agricultural Biotechnological Research Centre (MBK)GödöllőHungary
  3. 3.Department of AnatomyEötvös Loránd University (ELTE)BudapestHungary

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