Cereal Research Communications

, Volume 37, Issue 2, pp 151–158 | Cite as

Transposon-derived organ-specific mutation in the ADH1 RNA 5′ untranslated leader of autotetraploid maize (Zea mays L.)

  • M. Hajós-NovákEmail author
  • G. Dallmann
  • Z. Kristóf


A new unstable organ-specific Adh1 mutant was isolated from autotetraploid S 25 Wf9 maize line after germinating kernels under water for two days. Adh1-Fm4x-1 expresses normal and/or reduced levels of ADH1 in anaerobic scutellum. No activity or approximately 33–75% of wild type level of ADH1 in pollen grains was observed. The organ-specific phenotype of the Adh1-Fm4x-1 could be maintained by selfing for ten subsequent generations. After crossing with the wild-type allele the Adh1 mutation transmitted to the next generation both by female and male gametophytes. The appearance of one- and two-banded patterns in anaerobic scutellum and pollen grains of heterozygous F 1 plants showed the lack of F·F homodimers.

DNA sequence analyses of the wild type allele, the Adh1-Fm4x-1 mutant allele and four F 1 revertants revealed that the Adh1-Fm4x-1 mutation contains a Dissociation (Ds1) transposable element in the 5′ untranslated leader of the maize Adh1 gene, which regulates organ-specific expression at a quantitative level.


Ds1 element Adh1 autotetraploid maize unstable organ-specific mutation 5′ UTR point mutation 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Chen, C.-H., Freeling, M., Mercklebach, A. 1986. Enzymatic and morphological consequences of Ds excisions from maize Adh1. Maydica (McClintock issue) 31:93–108.Google Scholar
  2. Chen, C.-H., Olshi, K.K., Kloeckener-Gruissem, B., Freeling, M. 1987. Organ-specific expression of maize Adh1 is altered after a Mu transposon insertion. Genetics 116:469–477.PubMedPubMedCentralGoogle Scholar
  3. Davis, R.J. 1964. Disc electrophoresis 2. Method of application to human serum proteins. A.N.Y. Acid. Sci. 121:404–427.CrossRefGoogle Scholar
  4. Dawe, R.K., Lachmansingh, A.R., Freeling, M. 1993. Transposon-mediated mutations in the untranslated leader of maize Adh1 that increase and decrease pollen-specific gene expression. Plant Cell 5:311–319.PubMedPubMedCentralGoogle Scholar
  5. Freeling, M. 1976. Intragenic recombination in maize: Pollen analysis methods and the effect of parental Adh1 isoalleles. Genetics 83:701–717.PubMedPubMedCentralGoogle Scholar
  6. Freeling, M., Cheng, S.-K.D., Alleman, M. 1982. Mutant alleles that are altered in quantitative, organ-specific behavior. Dev. Genet. 3:179–196.CrossRefGoogle Scholar
  7. Hajósné, Novák M., H. Nagy, A. 1995. Az alkohol dehidrogenáz-1 (Adh1) gén kifejeződése tetraploid kukorica (Zea mays L.) pollenjében. [Expression of alcohol-dehydrogenase-1 (Adh1) gene in the pollen grains of autotetraploid maize (Zea mays L.)]. Növénytermelés 44:229–235.Google Scholar
  8. Kloeckner-Guissem, B., Vogel, J.M., Freeling, M. 1992. The TATA box promoter region of maize Adh1 affects its organ-specific expression. The EMBO Journal 11:157–166.CrossRefGoogle Scholar
  9. Schwartz, D. 1969. An example of gene fixation resulting from selective advantage in suboptimal conditions. Am. Nat. 103:479–481.CrossRefGoogle Scholar
  10. Schwartz, D., Osterman, J. 1976. A pollen selection system for alcohol-dehydrogenase negative mutants in plants. Genetics 83:63–65.PubMedPubMedCentralGoogle Scholar
  11. Sutton, W.D., Gerlach, W.L., Schwartz, D., Peacock, W.J. 1984. Molecular nature of Ds controlling element mutations at the Adh1 locus of maize. Science 223:1265–1268.CrossRefGoogle Scholar
  12. Williams, C.E., Kloeckener-Guissem, B., Freeling, M. 1991. Naturally occurring variants of maize Adh1 differ in organ-specific expression both in quantity and developmental timing. Maydica 36:115–128.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2009

Authors and Affiliations

  1. 1.Institute for Genetics and Biotechnology, Section of Plant BreedingSzent István University (SZIE)GödöllőHungary
  2. 2.Agricultural Biotechnological Research Centre (MBK)GödöllőHungary
  3. 3.Department of AnatomyEötvös Loránd University (ELTE)BudapestHungary

Personalised recommendations