PCR-Based Assays for the Identification of Fusarium spp. Originating from Wheat Grain

Abstract

The species-specific PCR assays correctly identified pure cultures of Fusarium acuminatum (3 isolates), F. avenaceum (22 isolates), F. poae (13 isolates), and F. sporotrichioides (6 isolates) originated from Hungarian wheat grain.

The PCR-based assays described in this study can be used for the routine detection and identification of above-mentioned Fusaria without morphological determination.

References

  1. Bottalico, A. 1998. Fusarium diseases of cereals: species complex and related mycotoxin profiles, in Europe. Journal of Plant Pathology 80:85–103.

    CAS  Google Scholar 

  2. Bottalico, A., Perrone, G. 2002. Toxigenic Fusarium species and mycotoxins associated with head blight in small-grain cereals in Europe. European Journal of Plant Pathology 108:611–624.

    CAS  Article  Google Scholar 

  3. Demeke, T., Clear, R.M., Patrick, S.K., Gaba, D. 2005. Species-specific PCR-based assays for the detection of Fusarium species and a comparison with the whole seed agar plate method and trichothecene analysis. International Journal of Food Microbiology 103:271–284.

    CAS  Article  Google Scholar 

  4. Desjardins, A.E. 2006. Fusarium Mycotoxins. Chemistry, Genetics and Biology. APS Press, St. Paul, Minnesota, USA. 260 pp.

    Google Scholar 

  5. Doohan, F.M., Parry, D.W., Jenkinson, P., Nicholson, P. 1998. The use of species-specific PCR-based assys to analyse Fusarium ear blight of wheat. Plant Pathology 47:197–205.

    CAS  Article  Google Scholar 

  6. Jurado, M., Vázquez, C., Patino, B., González-Jaén, M.T. 2005. PCR detection assays for the trichothecene-producing species Fusarium graminearum, Fusarium culmorum, Fusarium poae, Fusarium equiseti and Fusarium sporotrichioides. Systematic and Applied Microbiology 28:562–568.

    CAS  Article  Google Scholar 

  7. Jurado, M., Vázquez, C., Marin, S., Sanchis, V., González-Jaén, M.T. 2006. PCR-based srategy to detect contamination with mycotoxigenic Fusarium species in maize. Systematic and Applied Microbiology 29:681–689.

    CAS  Article  Google Scholar 

  8. Koncz, Zs., Huszti, K., Naár, Z., Kiss, A., Szécsi, Á. 2008. PCR identification of Fusarium graminearum isolated from wheat grain. Cereal Res. Comm. 36:623–630.

    CAS  Article  Google Scholar 

  9. McMullen, M.P., Enz, J., Lukach, J., Stover, R. 1997. Environmental conditions associated with Fusarium head blight epidemics of wheat and barley in the Northern Great Plains, North America. Cereal Res. Comm. 25:777–778.

    Google Scholar 

  10. Mulé, G., González-Jaén, M.T., Hornok, L., Nicholson, P., Waalwijk, C. 2005. Advances in molecular diagnosis of toxigenic Fusarium species. Food Additives and Contaminants 22:316–323.

    Article  Google Scholar 

  11. Niessen, L., Vogel, R.F. 1998. Group specific PCR-detection of potential trichothecene-producing Fusarium species in pure cultures and cereal samples. Systematic and Applied Microbiology 21:618–631.

    CAS  Article  Google Scholar 

  12. Niessen, L., Schmidt, H., Vogel, R.F. 2004. The use of tri5 gene sequences for PCR detection and taxonomy of trichothecene-producing species in the Fusarium section Sporotrichiella. International Journal of Food Microbiology 95:305–319.

    CAS  Article  Google Scholar 

  13. Parry, D.W., Jenkinson, P., McLeod, L. 1995. Fusarium ear blight (scab) in small grain cereals — A review. Plant Pathology 44:207–238.

    Article  Google Scholar 

  14. Parry, D.W., Nicholson, P. 1996. Development of a PCR assay to detect Fusarium poae in wheat. Plant Pathology 45:383–391.

    CAS  Article  Google Scholar 

  15. Turner, A.S., Lees, A.K., Rezanoor, H.N., Nicholson, P. 1998. Refinement of PCR-detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum. Plant Pathology 47:278–288.

    CAS  Article  Google Scholar 

  16. Williams, K.J., Dennis, J.T., Smyl, C., Wallwork, H. 2002. The application of species-specific assays based on the polymerase chain reaction to analyze Fusarium crown rot of durum wheat. Australasian Plant Pathology 31:119–129.

    Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Á. Szécsi.

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Cite this article

Koncz, Z., Magyar, D., Naár, Z. et al. PCR-Based Assays for the Identification of Fusarium spp. Originating from Wheat Grain. CEREAL RESEARCH COMMUNICATIONS 36, 631–636 (2008). https://doi.org/10.1556/CRC.36.2008.4.12

Download citation

Keywords

  • Fusarium acuminatum
  • F. avenaceum
  • F. poae
  • F. sporotrichioides
  • identification
  • PCR
  • species-specific primer
  • wheat grain