Molecular, Serological and Transmission Electron Microscopic Analysis of the Barley Yellow Dwarf Virus-PAV and the Cereal Yellow Dwarf Virus-RPV in Canary Seed (Phalaris canariensis L.)
Canary seed (Phalaris canariensis L.) is a cereal crop belonging to the tribe Phalarideae of the Poaceae family. A prevailing virus infection, which causes dwarfing of plants and yellowing of leaves, was observed in canary seed fields in the Tekirdag province of Turkey. The aim of this study was to identify, clone and sequence the cereal viruses naturally occurring on P. canariensis by employing serological tests as DAS-ELISA and TAS-ELISA tests combined with transmission electron microcopy (TEM) and molecular analysis. One hundred and one plant samples showing symptoms were collected and tested serologically using polyclonal antisera against Barley yellow dwarf virus -PAV (BYDV-PAV) and Cereal yellow dwarf virus-RPV (CYDV-RPV). The results of both immunoassays showed that 48% of the samples were infected with BYDV-PAV, 2% with CYDV-RPV and 14% were mixed infection. 36% of the samples were uninfected or infected at level below detection. Aphid transmission experiments revealed that barley (cv. Rubina) exhibited characteristic of CYDV-RPV. Investigations of infected canary grass seedlings using transmission electron microscopy (TEM) supported the findings of serological tests and revealed the presence of isometric particles of approximately 25 nm in diameter. These results were also confirmed by using BYDV-PAV and CYDV-RPV specific primers. Sequence analysis of cDNA and probable translation products revealed a high level of homology to BYDV-PAV and CYDV-RPV isolates found in other plant species. The sequence data obtained from this research were deposited in the EMBL/GenBank Data Libraries under the accession nos. EGO19056 and EF372272.
Keywordscereals viruses cDNA DAS-ELISA TAS-ELISA
- Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. 1996. Viruses of Plants, Descriptions and lists from the VIDE Database. Cab International, The University Press, Cambridge, U.K. 1484 pp.Google Scholar
- Catherall, P.L., Priestly, A.L., Chamberlain, J.A., A’Brook, J., Evans, H.M. 1983. Cereal and Grass Viruses. Welsh Plant Breeding, pp. 183–194.Google Scholar
- Falke, K.C., Friedt, W., Ordon, F. 2000. Nachweis der Expression von Bci-4 und LOX: 2 Hv1 in Gerste (Hordeum vulgare L.) nach DCINA Applikation. Justus Liebig Universitaet, Diplomarbeit.Google Scholar
- Ilbagi, H., Citir, A., Yorganci, U. 2005. Occurrence of virus infection on cereal crops and their identifications in the Trakya Region of Turkey. Journal of Plant Diseases and Protection 112:313–320.Google Scholar
- Mehner, S., Manurung, B., Gruntzig, M., Habekuss, A., Witsack, W., Fuchs, E. 2003. Investigations into the ecology of the Wheat dwarf virus (WDV) in Saxony-Anhalt, Germany. Journal of Plant Diseases and Protection 110:313–323.Google Scholar
- Milne, R.G. 1984. Electron microscopy for the identification of plant viruses in in vitro preparations. In: Maramorosh, K., Koprowski, H. (eds), Methods in Virology, Academic Press, New York. 7:88–116.Google Scholar
- Norton, R.H., Ford, J.F. 2002. Canary seed industry development for south-eastern Australia. A Report for the Rural Industries Research and Development Corporation. RIRDC Publication No. 1/178. 99 pp.Google Scholar
- Ozder, N., Toros, S. 1999. Tekirdag Ili’nde buǧdaylarda zarar yapan yaprak biti türlerinin saptanması üzerinde araştırmalar (Studies on the aphid species harmful to wheat cultivars in Tekirdag province). Türkiye Entomoloji Dergisi 23:101–110.Google Scholar
- Putnam, D.H., Oelke, E.A., Oplinger, E.S., Doll, J.D., Peters, J.B. 2000. Annual Canary Grass. The University of Michigan, Alternative Field Crops Manual, USA.Google Scholar
- Rochow, W.F. 1979. Comparative diagnosis of barley yellow dwarf by serological and aphid transmission tests. Plant Disease Reports 63:426–430.Google Scholar
- Rochow, W.F. 1982. Dependent transmission by aphids of barley yellow dwarf luteoviruses from mixed infections. Phytopathology 72:302–305.Google Scholar
- Vacke, J. 1974. Addition to the study on host plants of Oat sterile dwarf virus (in Russian). Vedecke Prace Vyzkumnych Ustavu Rostlinne Vyroby v Praze-Ruzyni 18:81–85.Google Scholar
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