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Cereal Research Communications

, Volume 36, Issue 2, pp 327–332 | Cite as

Evaluation of Genetic Variation in High Molecular Weight Glutenin Subunits of Seed Storage Protein Using Landraces of Common Wheat from Pakistan

  • K. NiwaEmail author
  • H. Suzuki
  • T. Tominaga
  • S. Nasim
  • R. Anwar
  • M. Ogawa
  • Y. Furuta
Open Access
Article

Abstract

SDS-PAGE was used to determine the variation in high molecular weight glutenin subunits (HMW-GS) alleles present in 170 landraces of common wheat from Sindh, Balochistan and NWFP in Pakistan. Three alleles for Glu-A1, six alleles for Glu-B1 and four alleles for Glu-D1 were detected. These included three new alleles, namely Glu-B1e, Glu-B1h and Glu-D1n, which had not previously been found in landraces of Pakistani wheat. Furthermore, Glu-D1m and Glu-D1n, which are considered to be endemic alleles in Pakistan and Afghanistan, were both detected from Balochistan. Twenty genotypes were identified based on combinations of alleles at the three Glu-1 loci. The present study showed that Pakistani landraces of common wheat have maintained a broad diversity of HMW-GS alleles, and that they may serve as genetic resources to improve wheat varieties adapted to Pakistani climatic and edaphic conditions.

Keywords

common wheat glutenin HMW-GS Pakistan 

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Copyright information

© Akadémiai Kiadó, Budapest 2008

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • K. Niwa
    • 1
    Email author
  • H. Suzuki
    • 1
  • T. Tominaga
    • 2
  • S. Nasim
    • 3
  • R. Anwar
    • 3
  • M. Ogawa
    • 4
  • Y. Furuta
    • 5
  1. 1.Department of AgricultureTokyo University of AgricultureAtsugi, KanagawaJapan
  2. 2.Graduate School of AgricultureKyoto UniversityKyotoJapan
  3. 3.National Agricultural Research CenterPlant Genetic Resources InstituteIslamabadPakistan
  4. 4.Department of Human EnvironmentYamaguchi Prefectural UniversitySakurabatake, YamaguchiJapan
  5. 5.Faculty of Applied Biological SciencesGifu UniversityYanagido, GifuJapan

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