SDS-PAGE was used to determine the variation in high molecular weight glutenin subunits (HMW-GS) alleles present in 170 landraces of common wheat from Sindh, Balochistan and NWFP in Pakistan. Three alleles for Glu-A1, six alleles for Glu-B1 and four alleles for Glu-D1 were detected. These included three new alleles, namely Glu-B1e, Glu-B1h and Glu-D1n, which had not previously been found in landraces of Pakistani wheat. Furthermore, Glu-D1m and Glu-D1n, which are considered to be endemic alleles in Pakistan and Afghanistan, were both detected from Balochistan. Twenty genotypes were identified based on combinations of alleles at the three Glu-1 loci. The present study showed that Pakistani landraces of common wheat have maintained a broad diversity of HMW-GS alleles, and that they may serve as genetic resources to improve wheat varieties adapted to Pakistani climatic and edaphic conditions.
Anwar, R., Masood, S., Khan, M.A., Nasim, S. 2003. The high-molecular-weight glutenin subunit composition of wheat (Triticum aestivum L.) landraces from Pakistan. Pak. J. Bot. 35:61–68.
Bietz, J.A., Shepherd, K.W., Wall, J.S. 1975. Single-kernel analysis of glutenin: Use in wheat genetics and breeding. Cereal Chem. 52:513–532.
Furuta, Y., Anwar, R., Tominaga, T., Niwa, K., Nasim, S. 2001. A preliminary report of the Gifu University scientific exploration in Pakistan in 2001 (GSEE01). Fac. Agr., Gifu Univ., Gifu, Japan. pp. 1–23.
Lagudah, E.S., Flood, R.G., Halloran, G.M. 1987. Variation in high molecular weight glutenin subunits in landraces of hexaploid wheat from Afghanistan. Euphytica 36:3–9.
Lawrence, G.J., Shepherd, K.W. 1980. Variation in glutenin protein subunits of wheat. Aust. J. Biol. Sci. 33:221–233.
Masood, M.S., Asghar, M., Anwar, R. 2004. Genetic diversity in wheat landraces from Pakistan based on polymorphism for high molecular weight glutenin subunits (HMW-GS). Pak. J. Bot. 36:835–843.
McIntosh, R.A., Hart, G.E., Devos, K.M., Gale, M.D., Rogers, W.J. 1998. Catalogue of gene symbols for wheat. In: Proc. 9 th Int. wheat Genet. Symp. Vol. 5, Univ. Ext. Press, Univ. Saskatchewan, Saskatchewan, pp. 99–108.
MirAli, N., Arabi, M.I.E., Al-Safadi, B. 1999. High molecular weight glutenin subunits composition of Syrian grown bread wheat and its relationships with gluten strength. J. Genet. & Breed. 53:237–245.
Morgunov, A.I., Peña, R.J., Crossa, J., Rajarm, S. 1993. Worldwide distribution of Glu-1 alleles in bread wheat. J. Genet. & Breed. 47:53–60.
Nakamura, H. 2002. The geographical diversity of the frequency of the Glu-D1f allele in Asian common wheat, and the transmission route through which the wheat may have reached Japan. Aust. J. Agr. Res. 53: 1265–1269.
Payne, P.I., Holt, L.M., Law, C.N. 1981. Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Part 1: Allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor. Appl. Genet. 60:229–236.
Payne, P.I., Law, C.N., Mudd, E.E. 1980. Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor. Appl. Genet. 58:113–120.
Payne, P.I., Lawrence, G.J. 1983. Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res. Comm. 11:29–35.
Shewry, P.R. 2003. Wheat gluten proteins. In: Shewry, P.R., Lookhart, G.L. (eds), Wheat Gluten Protein Analysis. Amer. Assoc. Cereal Chem., Minnesota, pp. 1–17.
Tahir, M., Hussain, S.A., Turchetta, T., Lafiandra, D. 1995. The HMW glutenin subunit composition of bread-wheat varieties bred in Pakistan. Plant Breed. 114:442–444.
Tahir, M., Turchetta, T., Anwar, R., Lafiandra, D. 1996. Assessment of genetic variability in hexaploid wheat landraces of Pakistan based on polymorphism for HMW glutenin subunits. Genet. Res. Crop Evol. 43:211–220.
Tominaga, T., Niwa, K., Furuta, Y., Nasim, S., Anwar, R. 2002. Germplasm collection of the tribe Triticeae in Pakistan. Jpn. J. Trop. Agr. 46:302–306.
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Niwa, K., Suzuki, H., Tominaga, T. et al. Evaluation of Genetic Variation in High Molecular Weight Glutenin Subunits of Seed Storage Protein Using Landraces of Common Wheat from Pakistan. CEREAL RESEARCH COMMUNICATIONS 36, 327–332 (2008). https://doi.org/10.1556/CRC.36.2008.2.12
- common wheat