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Cereal Research Communications

, Volume 36, Issue 2, pp 201–213 | Cite as

Validity of Selected DNA Markers for Breeding Leaf Rust Resistant Wheat

  • L. BłaszczykEmail author
  • I. Kramer
  • F. Ordon
  • J. Chełkowski
  • M. Tyrka
  • G. Vida
  • I. Karsai
Article

Abstract

The set of 44 near-isogenic lines of spring wheat cv. Thatcher and wheat genotypes known to carry specific leaf rust resistance genes were included in the present study for the preliminary validation of twelve STS and SCAR markers linked to leaf rust resistance genes Lr9, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26, Lr28, Lr29, Lr37. Seven Lr genes were specifically tagged by STS and SCAR markers. The presence of genes Lr9, Lr19, Lr20, Lr24, Lr28, Lr29, Lr37 in the tested plant materials was confirmed by a unique amplification of markers SCS5 550, SCS265 512 and SCS253 736, STS638, SCS73 719, SCS421 570, IPY10 and Lr29F24/R24, cslVrgal3, PS10R/L, respectively. Evaluation of the repeatability and the reliability of selected markers (pTAG621 for Lr1, STS683 for Lr20, D14L for Lr21, Lr25F20/R19 for Lr25, Lr29F24/R24, IPY10 for Lr29, cslVrgal3 for Lr37 and PS10R/L for Lr47) across four European laboratories and PCR conditions demonstrated the usability of STS638, Lr29F24/R24, IPY10, cslVrgal3 and PS10R/L markers in marker-assisted selection. STS markers pTAG621 for gene Lr1, D14L for gene Lr21, Lr25F20/R19 for gene Lr25 were found to be unsuitable for effective screening of large segregating populations in breeding programs.

Keywords

DNA markers wheat leaf rust resistance genes 

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Copyright information

© Akadémiai Kiadó, Budapest 2008

Authors and Affiliations

  • L. Błaszczyk
    • 1
    Email author
  • I. Kramer
    • 2
  • F. Ordon
    • 2
  • J. Chełkowski
    • 1
  • M. Tyrka
    • 3
  • G. Vida
    • 4
  • I. Karsai
    • 4
  1. 1.Institute of Plant Genetics Polish Academy of SciencesPoznańPoland
  2. 2.Federal Centre for Breeding Research on Cultivated PlantsInstitute of Epidemiology and Resistance ResourcesQuedlinburgGermany
  3. 3.Laboratory for Population GeneticsPolonia UniversityCzȩstochowaPoland
  4. 4.Agricultural Research Institute of the Hungarian Academy of SciencesMartonvásárHungary

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