Cereal Research Communications

, Volume 36, Issue 1, pp 33–42 | Cite as

Introgression of Wheat Chromosomes into Diploid Rye by Use of a Hexaploid Triticale with an ABRRRR Genome

  • B. ApolinarskaEmail author


The objective of this study was to continue attempts to introduce wheat chromosomes, particularly those from the B genome, into diploid rye. An allohexaploid having 2 wheat mixogenomes (1B, 2A, 3B, 4B, 5B, 6A and 7B) and 4 rye genomes (RRRR) was crossed with substitution 2× rye containing the chromosomes of the wheat Agenome except 3A, and next backcrossed with substitution rye. Karyotypes were analysed by C-banding in the produced plants of the generations F 1, BC 1 -F 1, and BC 1 -F 2. In nearly all plants of the F 1 generation (except one), 4–12 wheat chromosomes were found, mainly those of the B genome. A comparison of 2 successive generations indicates that both the mean and range of numbers of wheat chromosomes in the offspring of substitution plants and substitution-addition plants changed sometimes to the advantage of wheat chromosomes but sometimes to their disadvantage. A decline was observed in the contribution of B chromosomes and of chromosomes 2A and 6A, but pollen introduced some wheat chromosomes from the male parent: 1A, 4A, 5A and 7A. Wheat B chromosomes contributed to disturbances in plant development (lack of the spike emergence stage), but usually caused spike sterility, and even the single grains produced were usually unable to germinate. As a result, wheat chromosomes of the B genome were finally completely eliminated from the analysed material. The presence of wheat chromosomes of the A genome in fertile 2× rye plants, as well as their transfer to the next generations, indicate that the A genome is more closely related to the rye genome than the B genome. Positive introgression of wheat chromatin from the A genome into 2× rye depends to a large extent on chromosome engineering by means of appropriate crossing combinations, as A chromosomes from the male parent were much better tolerated than those from the female parent.


diploid rye genome wheat chromosome substitution addition 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Apolinarska, B. 1996a. Different chromosome combinations on tetra- and hexaploid level from hybrids of tetraploid rye × tetraploid triticale. In: Guedes-Pinto, H., Darvey, N., Carnide, P.V. (eds), Triticale: Today and Tomorrow. Developments in Plant Breeding. Kluwer Acad. Publ., Netherlands, pp. 189–194.CrossRefGoogle Scholar
  2. Apolinarska, B. 1996b. Transfer of chromosomes of the A and B genomes of wheat to tetraploid rye. J. Appl. Genet. 37:345–356.Google Scholar
  3. Apolinarska, B. 1996c. Additions, substitutions and translocations of wheat chromosomes in tetraploid rye. Inter. Symp. Rye Breed. Genet. 27–29 June, Stuttgart, Germany. Vortr. Pflanzenzüchtung. 35:304–305.Google Scholar
  4. Apolinarska, B. 1998: Tetraploid rye with wheat genetic material. In: Maluszyńska, J. (ed.), Plant Cytogenetics. Spring Symp., Cieszyn 19–22 May 1997, Silesian Univ. Katowice, Poland, pp. 206–209.Google Scholar
  5. Apolinarska, B. 2003a. Substitutions, additions and translocations of wheat chromosomes into diploid rye. Bull. Plant Breed. and Acclim. Inst. 230:195–203.Google Scholar
  6. Apolinarska, B. 2003b. Chromosome pairing in tetraploid rye with monosomic-substitution wheat chromosomes. J. Appl. Genet. 44:119–128.Google Scholar
  7. Apolinarska, B. 2006. Chromosome pairing in diploid substitution rye and addition rye with wheat chromosomes. Cereal Res. Comm. 34:1223–1229.CrossRefGoogle Scholar
  8. Apolinarska, B., Wojciechowska, B. 2003. Production of diploid rye/wheat chromosome additions and substitutions. Cereal Res. Comm. 31:73–79.Google Scholar
  9. Baum, M. 1991: Rye-wheat hybrids: the production of wheat chromosome additions to rye. Genome 34:840–844.CrossRefGoogle Scholar
  10. Boros, D., Lukaszewski, A.J., Aniol, A. 2001. Location of genes controlling the content of dietary fibre and arabinoxylans in rye. Proc. Eucarpia Rye Meet. July 4–7, 2001, Radzików, Poland, p. 285.Google Scholar
  11. Łapiński, B., Rafalski, A. 2001. Tetraploid triticale as a source of new variation for rye breeding. Proc. Eucarpia Rye Meet. July 4–7, 2001, Radzików, Poland, pp. 157–164.Google Scholar
  12. Lukaszewski, A.J., Brzezińki, W., Klockiewicz-Kamińka, E. 2000: Transfer of the Glu-D1 locus encoding high molecular weight glutenin subunits 5+10 from breadwheat to diplod rye. Euphytica 115:49–57.CrossRefGoogle Scholar
  13. Lukaszewski, A.J., Gustafson, P.J. 1983: Translocations and modifications of chromosomes in triticale × wheat hybrids. Theor. Appl. Genet. 64:239–248.CrossRefGoogle Scholar
  14. Melz, G., Thiele, V., Seidel, A., Buschbeck, R. 1991. Rye-cytoplasmic rye-wheat additions — a new material for breeding. Genet. Polonica 32:89–93.Google Scholar
  15. Rakowska, M. 1994. Antinutritive compounds in rye grain. Plant Breed. Acclimat. and Seed Prod. 38:21–42.Google Scholar
  16. Schlegel, R. 1982: First evidence for rye — wheat additions. Biol. Zbl. 101:641–646.Google Scholar
  17. Thiele, V., Buschbeck, R., Seidel, A., Melz, G. 1988: Identification of the first rye-cytoplasmic rye-wheat-addition using LAP-isozymes. Plant Breed. 101:250–252.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2008

Authors and Affiliations

  1. 1.Polish Academy of Sciences Institute of Plant GeneticsPoznańPoland

Personalised recommendations