Evaluation of Genetic Diversity in the Subspecies of Aegilops tauschii Using Microsatellite Markers
Twenty four SSR primer pairs were used to determine the genetic diversity in the subspecies of Aegilops tauschii. All microsatellite markers showed a high level of polymorphism. The average number of alleles observed was relatively similar in subsp. tauschii (9.00) to that in strangulata (8.30). The tauschii group had also similar average PIC value (0.80) with the strangulata group (0.77). The range of similarity was higher in subsp. tauschii than subsp. strangulata, reflecting a higher level of genetic variation in the first subspecies. Cluster and PCO analyses revealed a relative pattern of differentiation between two taxonomic classified subspecies, but there were some exceptional accessions in each group, reflecting possible intermediate forms between these two subspecies. Wide distribution of alleles found in both subspecies can be used for wheat improvement.
KeywordsAegilops tauschii genetic diversity germplasm microsatellite
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