Polymorphism Analysis Using 1RS-Specific Molecular Markers in Rye Cultivars (Secale cereale L.) of Various Origin

Abstract

Six different 1RS-specific molecular markers (RMS13, Bmac213, GPI, 5S, SCM9, IAG95) were tested in twenty rye cultivars of various origin. The aim of the experiments was to choose rye cultivars which give polymorphic PCR products with these 1RS-specific markers compared to the wheat cultivar Mv Magdaléna, which contains the 1BL.1RS translocation. The polymorphic rye cultivars can be presumed to differ from the 1BL.1RS translocation originating from the Petkus rye cultivar and will hopefully carry effective resistance genes which can be incorporated into the 1BL.1RS translocation in wheat. Twenty rye cultivars (at least two plants per cultivar) were analysed with these markers. Of fifty-two rye samples analysed, three plants were found to be polymorphic, one (Kisvárdai Alacsony from Hungary), for the 5S marker, one (Kriszta from Hungary) for the RMS13 marker and one (Porto from Portugal) for the SCM9 marker. The polymorphic plants were grown to maturity in the phytotron.

References

  1. Bedő, Z., Balla, L., Szunics, L., Láng, L., Kramarik Kissimon, J. 1993. A martonvásári 1B/1R transzlokációs búzafajták agronómiai tulajdonságai. (Agronomic properties of Martonvásár wheat varieties bearing the 1B/1R translocation). Növénytermelés 42:391–398.

    Google Scholar 

  2. Börner, A., Korzun, V. 1998. A consensus linkage map of rye (Secale cereale L.) including 374 RFLPs, 24 isozymes and 15 gene loci. Theor. Appl. Genet. 97:1279–1288.

    Article  Google Scholar 

  3. Graybosch, R.A. 2001. Uneasy unions: quality effects of rye chromatin transfers to wheat. J. Cereal Sci. 33:3–16.

    CAS  Article  Google Scholar 

  4. Hackauf, B., Wehling, P. 2002. Identification of microsatellite polymorphisms in an expressed portion of the rye genome. Plant Breeding 121:17–25.

    CAS  Article  Google Scholar 

  5. Khlestkina, E.K., Than, M.H.M., Pestsova, E.G., Röder, M.S., Malyshev, S.V., Korzun, V., Börner, A. 2004. Mapping of 99 new microsatellite loci in rye (Secale cereale L.) including 39 expressed sequence tags. Theor. Appl. Genet. 109:725–732.

    CAS  Article  Google Scholar 

  6. Koebner, R.M.D. 1995. Generation of PCR-based markers for detection of rye chromatin in a wheat background. Theor. Appl. Genet. 90:740–745.

    Article  Google Scholar 

  7. Korzun, V., Malyshev, S., Kartel, N., Westermann, T., Weber, W.E., Börner, A. 1998. A genetic linkage map of rye (Secale cereale L.). Theor. Appl. Genet. 96:203–208.

    CAS  Article  Google Scholar 

  8. Korzun, V., Malyshev, S., Voylokov, A.V., Börner, A. 2001. A genetic map of rye (Secale cereale L.) combining RFLP, isozyme, protein, microsatellite and gene loci. Theor. Appl. Genet. 102:709–717.

    CAS  Article  Google Scholar 

  9. Kotvics, G., Krisztián, J., Dornbach, L. 1999. Perennial cultivated rye; a new variety for Hungarian crop production. Agrofórum X. 5.63.

  10. Kruppa, J. 2001. Results obtained in the breedup and production of rye and triticale. PhD Thesis, Department of Agriculture, Centre of Agricultural Sciences, University of Debrecen.

  11. Kőszegi, B., Linc, G., Juhász, A., Láng, L., Molnár-Láng, M. 2000. Occurrence of the 1RL/1BL wheat-rye translocation in Hungarian wheat varieties. Acta Agron. Hung. 48:227–236.

    Article  Google Scholar 

  12. Ma, X.F., Wanous, M.K., Houchins, K., Rodriguez Milla, M. A., Goicoechea, P.G., Wang, Z., Xie, M., Gustafson, J.P. 2001. Molecular linkage mapping in rye (Secale cereale L.). Theor. Appl. Genet. 102:517–523.

    CAS  Article  Google Scholar 

  13. Mago, R., Spielmeyer, W., Lawrence, G., Lagudah, E., Ellis, J., Pryor, A. 2002. Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines. Theor. Appl. Genet. 104:1317–1324.

    CAS  Article  Google Scholar 

  14. Masojc, P., Myskow, B., Milczarski, P. 2001. Extending a RFLP-based genetic map of rye using random amplified polymorphic DNA (RAPD) and isozyme markers. Theor. Appl. Genet. 102:1273–1279.

    CAS  Article  Google Scholar 

  15. McIntosh, R.A., Yamazaki, Y., Devos, K.M., Dubcovsky, J., Rogers, W.J., Appels, R. 2003. Catalogue of gene symbols for wheat. In: Pogna, N.E., Romano, M., Pogna, E.A., Galterio, G. (eds), Proc. 10 th Intern. Wheat Genet. Symp. Vol. 4, Paestum, Italy, pp. 34.

  16. Mohler, V., Hsam, S.L.K., Zeller, F.J., Wenzel, G. 2001. An STS marker distinguishing the rye-derived powdery mildew resistance alleles at the Pm8/Pm17 locus of common wheat. Plant Breeding 120:448–450.

    CAS  Article  Google Scholar 

  17. Molnár-Láng, M., Linc, G., Sutka, J. 1996. Transfer of the recessive crossability allele kr1 from Chinese Spring into winter wheat variety Martonvásári 9. Euphytica 90:301–305.

    Article  Google Scholar 

  18. Nagy, E.D., Christoph, E., Molnár-Láng, M., Lelley, T. 2003a. Genetic mapping of sequence-specific PCR-based markers on the short arm of the 1BL.1RS wheat rye translocation. Euphytica 132:243–250.

    CAS  Article  Google Scholar 

  19. Nagy, E.D., Lelley, T. 2003b. Genetic and physical mapping of sequence specific amplified polymorphic (SSAP) markers on the 1RS chromosome arm in a wheat background. Theor. Appl. Genet. 107:1271–1277.

    CAS  Article  Google Scholar 

  20. Nagy, E.D., Molnár-Láng, M. 2000. Frequency of pairing between the 1B/1R translocation and its respective homo(eo)logues in a wheat-rye hybrid as revealed by GISH. Cereal Res. Comm. 28:41–48.

    Google Scholar 

  21. Rajaram, S., Mann, C.E., Oniz-Ferrara, G., Mujeeb-Kazi, A. 1983. Adaptation, stability and high yield potential of certain 1B/1R CIMMYT wheats. In: Sakamoto, S. (ed.), Proc. of the 6 th Intern. Wheat Genet. Symp., Kyoto, Japan, pp. 613–621.

  22. Ramsay, L., Macaulay, M., degli Ivanissevich, S., MacLean, K., Cardle, L., Fuller, J., Edwards, K.J., Tuvesson, S., Morgante, M., Massari, A., Maestri, E., Marmiroli, N., Sjakste, T., Ganal, M., Powell, W., Waugh, R. 2000. A simple sequence repeat-based linkage map of barley. Genetics 156:1997–2005.

    CAS  PubMed  PubMed Central  Google Scholar 

  23. Röder, M.S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M.H., Leroy, P., Ganal, M.W. 1998. A microsatellite map of wheat. Genetics 149:2007–2023.

    PubMed  PubMed Central  Google Scholar 

  24. Saal, B., Wricke, G. 1999. Development of simple sequence repeat markers in rye (Secale cereale L.). Genome 42:964–972.

    CAS  Article  Google Scholar 

  25. Schlegel, R., Korzun, V. 1997. About the origin of 1RS.1BL wheat-rye chromosome translocations from Germany. Plant Breed. 116:537–540.

    Article  Google Scholar 

  26. Shepherd, K.W. 1973. Homeology of wheat and alien chromosomes controlling endosperm protein phenotypes. In: Sears, E.R., Sears, L.M.S. (eds), Proc. 4 th Intern. Wheat Genet. Symp., Univ, Missouri, Columbia, USA, pp. 745–760.

  27. van Capenhout, S. 1997. Chromosome-specific PCR markers for wheat genome analysis and manipulation. Dissertationes de Agricultura 337. Katholike Universität, Leuven

    Google Scholar 

  28. Zeller, F.J. 1973. 1B/1R wheat-rye chromosome substitutions and translocations. In: Sears, E.R., Sears, L.M.S. (eds), Proc. 4 th Intern. Wheat Genet. Symp., Univ, Missouri, Columbia, USA, pp. 209–221.

  29. Zeller, F.J., Fuchs, E. 1983. Cytologie und Krankheitresistenz einer 1A/1R und mehrerer 1B/1R Weizen-Roggen Translokationssorten. Z. Pflanzenzüchtung 90:285–296.

    Google Scholar 

  30. Zeller, F.J., Hsam, F.L.K. 1984. Broadening the genetic variability of cultivated wheat by utilizing rye chromatin. In: Sakamoto, S. (ed.), Proc. 6 th Intern. Wheat Genet. Symp., Kyoto, Japan, pp. 161–173.

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Correspondence to M. Molnár-Láng.

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Schneider, A., Molnár-Láng, M. Polymorphism Analysis Using 1RS-Specific Molecular Markers in Rye Cultivars (Secale cereale L.) of Various Origin. CEREAL RESEARCH COMMUNICATIONS 36, 11–19 (2008). https://doi.org/10.1556/CRC.36.2008.1.2

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Keywords

  • wheat
  • rye
  • 1BL.1RS translocation
  • 1RS-specific molecular markers
  • polymorphism