The objectives of the study were to test diversity among winter barley breeding lines exhibiting various sensitivity to FHB and to find RAPD markers and AFLP markers that will distinguish between susceptible and moderately resistant genotypes. A test of a set of winter barley genotypes artificially infected in field trials by fusaria was carried out. Based on the results from field and laboratory evaluation and deoxynivalenol (DON) content assessment, barley genotypes with different responses to FHB were selected. The genotypes were hybridized and doubled haploid (DH) lines were derived in F1 generation using the in vitro androgenesis method. Initial parental components and derived DH lines were tested for FHB infection and DON content. A set of parental genotypes of winter barley was tested with 80 RAPD markers. Based on analyses of 80 RAPD primers in a set of parental genotypes of winter barley, the primer H15 was selected that provides specific product of 650-bp size for moderately resistant winter barley genotypes. In consecutive detection, this specific product was found in 4 DH lines. During the study, some DH lines were selected that exhibited improved resistance to Fusarium infection. A low infection level and low DON content were found in the winter barley line DH 610 from the combination of Br2611m × Duet. The AFLP technique was used to analyze parental genotypes of winter barley as well as 7 selected DH lines of winter barley. The detected markers can be further evaluated and employed to select breeding materials.
Agrama, H.A., Dahleen, L.S., McArthur, R.I. 2004. Cloning of AFLP Fragments Linked to Resistance Genes for Fusarium Head Blight in Barley. In: Proceedings of the 9 th International Barley Genetics Symposium, 20.–26. 6. 2004, Brno, pp. 760–762.
Armstrong, K., Ho, K.M., Fedak, G., Martin, R., Butler, G., Savard, M., Xue, A., Langille, L., Sabo, F., Kuc, M., Burville, M. 2001. A Study to Identify Molecular Markers for Fusarium Head Blight in Six-Row Barley. In: Fedak, G., Choo, A. (eds), Proc. 2 nd Canadian Workshop on Fusarium Head Blight, 3–5 November 2001, Ottawa, Ontario, pp. 22–25.
Buerstmayr, H., Legzdina, L., Steiner, B., Lemmens, M. 2004. Variation for Resistance to FHB in Spring Barley. In: Proceedings of the 9 th International Barley Genetics Symposium, 20.–26. 6. 2004, Brno, pp. 308–315.
Geddes, J., Eudes, F., Legge, B., Tucker, J. 2005. Assessment of Artificial Inoculation Methods and Deoxynivalenol Levels in Barley Lines Representing Various Candidate Sources of Resistance to Fusarium Head Blight. In: 18 th North American Researchers Workshop and 4 th Canadian Barley Symp., July 18–20, 2005, Red Deer, Alberta. Barley Newsletter 48:20.
Grewal, T.S., Rossnagel, B.G., Scoles, G.J. 2005. PCR Detection and Quantification of Fusarium Species. In: 18 th North American Researchers Workshop and 4th Canadian Barley Symp., July 18–20, 2005, Red Deer, Alberta. Barley Newsletter 48:6.
Hou, L., Ullrich, S.E., Kleinhofs, A. 1994. Inheritance of anther culture traits in barley. Crop Science 34:1243–1247.
Kolb, F.L., Bai, G.H., Muehlbauer, G.J., Anderson, J.A., Smith, K.P., Fedak, G. 2001. Host plant resistance genes for Fusarium Head Blight: Mapping and manipulation with molecular markers. Crop Science 41:611–619.
Lemmens, M., Josephs, R., Schumacher, R., Grausgruber, H., Buerstmayr, H., Ruckenbauer, P., Heuhold, G., Fidesser, M., Krska, R. 1997. Head Blight (Fusarium spp.) on Wheat: Investigation on the Relationship Between Disease Symptoms and Mycotoxin Content. In: Mesterházy, Á. (ed.), Proceedings of the 5 th European Fusarium Seminar, 29.08.–5.09.1997, Szeged, pp. 459–465.
Machii, H., Mizuno, H., Hirabayashi, T., Li, H., Hagio, T. 1998. Screening wheat genotypes for high callus induction and regeneration capability from anther and immature embryo cultures. Plant Cell, Tissue and Organ Culture 53:67–74.
Maier, Ch., Schmierer, D., Drader, T., Horsley, R., Kleinhofs, A. 2005. Saturation Mapping of Barley Chromosome 2H Fusarium Head Blight Resistance QTL. In: 18 th North American Researchers Workshop and 4 th Canadian Barley Symp., July 18–20, 2005, Red Deer, Alberta. Barley Newsletter 48:40.
Nei, M., Li, W.H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Nat. Acad. Sci. USA 76:5269–5273.
Saghai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A., Allard, R.W. 1984. Ribosomal DNA Spacer-length Polymorphisms in Barley: Mendelian Inheritance, Chromosomal Location and Population Dynamics. In: Proceedings of National Academy Sciences, 81:8014–8018.
Steffenson, B.J. 2003. Fusarium Head Blight of Barley. In: Leonard, K.J., Bushnell, W.R. (eds), Fusarium Head Blight of Wheat and Barley. APS Press, St. Paul, Minnesota, USA, pp. 241–295.
Spunarova, M., Ovesna, J., Tvaruzek, L., Kucera, L., Spunar, J., Hollerova, I. 2005. The Use of molecular markers for characterisation of spring barley for breeding to Fusarium Head Blight. Plant, Soil, Environment 51:483–490.
Takeda, K., Heta, H. 1989. Establishing the testing method and a search for the resistant varieties to Fusarium Head Blight in barley. Japan. J. Breed. 39:203–216.
Tvaruzek, L., Ji, L., Cao, K. 2003. Reaction of Winter Wheat Genotypes from Chinese and Czech Collection to Fusarium Head Blight and Leaf Diseases. In: Dagun, Liu (ed.), Research of Plant Pathology in Hebei. China Agriculture Press 1, pp. 94–100.
Vagera, J., Ohnoutkova, L. 1993. In vitro production of and rogenesis in wheat and barley. Rostlinna vyroba 39:97–114.
Zhu, Z., Cao, W., Lu, W., Fedak, G., Armstrong, K. 2001. Identification of AFLP Marker for Fusarium Head Blight Resistance in Wheat. In: Fedak, G., Choo, A. (eds), Proc. 2 nd Canadian Workshop on Fusarium Head Blight, 3–5 November 2001, Ottawa, Ontario, pp. 44–45.
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Nesvadba, Z., Vyhnanek, T., Jeziskova, I. et al. The use of RAPD and AFLP markers for Characterisation of Winter Barley Genotypes for Breeding to Fusarium Head Blight Resistance. CEREAL RESEARCH COMMUNICATIONS 36, 1–10 (2008). https://doi.org/10.1556/CRC.36.2008.1.1
- winter barley
- Fusarium head blight
- doubled haploid lines