Information on genetic diversity of maize hybrids is very important for germplasm enhancement, hybrid breeding and in preventing environmental damage which may occur due to genetic uniformity of hybrids grown on large areas. The main goal of our study was to assess temporal changes in genetic diversity over the past four decades among ZP maize hybrids within the largest planting area in Serbia. The objectives were to: (i) characterize maize hybrids from different periods by RAPD markers; (ii) analyze temporal changes in genetic diversity among maize hybrids. Twenty-four maize hybrids were chosen from breeding programs in Maize Research Institute “Zemun Polje” on the basis of their superior yield and predominant seed production throughout four Periods. Patterns of RAPD markers were unique for each studied genotype. The average GD between hybrids continuously increased from Period B to Period E. Changes in genetic background during the last 40 years have a major impact on genetic diversity among ZP maize hybrids. There was no significant change in genetic variability of hybrids through the Periods.
Drinić, G., Stankovic, G., Pajic, Z., Vancetovic, J., Ignjatović Micic, D. 2006. Sixty years of ZP maize hybrid breeding. Maydica, Vol. 51 (in press).
Duvick, D.N., Smith, J.S.C., Cooper, M. 2004. Changes in performance, parentage and genetic diversity of successful corn hybrids, 1930–2000. In: Smith, C.W., Betran, J., Runge, E.C.A. (eds), Corn: Origin, History, Technology and Production. Wiley, New York, pp. 65–97.
Hadi, G. 2006. Genetic basis of maize production in Eastern Central Europe between 1610 and 2005. Review. Cereal Research Communications 34:1307–1314.
Hadi, G., Marton, L.C., Szundy, T., Kovács, I., Pintér, J., Dolinka, B. 2004. Contribution made by the maize variety Mindszentpusztai Yellow Dent (MYD) to the birth of hybrid maize in Hungary and in Europe as a whole. Review. Cereal Research Communications 32: 159–166.
Heun, M., Helentjaris, T. 1993. Inheritance of RAPDs in F1 hybrids of corn. Theor. Appl. Genet. 85: 961–968.
Jaccard, P. 1908. Nouvelles rescherches sur la distribution floral. Bull. Soc. Vand. Sci. Nat. 44:223–270.
Lamkey, K.R., Smith, O.S. 1987. Performance and inbreeding depression of population representing seven eras of maize breeding. Crop Sci. 35:695–699.
Le Clerc, V., Bazante, F., Baril, C., Guiard, J., Zhang, D. 2005. Assessing temporal changes in genetic diversity of maize varieties using microsatellite markers. Theor. Appl. Genet. 110:294–302.
Lu, H., Bernardo, R. 2001. Molecular marker diversity among current and historical maize inbreds. Theor. Appl. Genet. 103:613–617.
Melchinger, A., Messmer, M.M., Lee, M., Woodman, W.L., Lamkey, K.R. 1991. Diversity and relationships among U.S. maize inbreeds revealed by restriction fragment length polymorphisms. Crop Sci. 31:669–678.
Nagy, E., Marton, L.C. 2006. The use of morphological traits and genetic markers to estimate genetic relationships in maize. Cereal Research Communications 34:887–894.
Reif, J., Hamrit, S., Heckenberger, M., Schipprack, W., Maurer, P., Bohn, M., Melchinger, A. 2005. Trends in genetic diversity among European maize cultivars and their parental components during the past 50 years. Theor. Appl. Genet. 111:838–845.
Rogers, S.O., Bendich, A.J. 1985. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol. Biol. 5:69–76.
Rohlf, F.J. 2000. NTSYS-pc. Numerical taxonomy and multivariate analysis system. Version 2.0 Exeter Software, Setaket, N.Y.
Smith, J.S.C. 1989. The characterization and assessment of genetic diversity among maize hybrids that are widely grown in France: Chromatographic and isozyme date. Euphytica 43:73–85.
Smith, J.S., Duvick, D., Smith, O.S., Cooper, M., Feng, L. 2004. Changes in pedigree backgrounds of pioneer brand maize hybrids widely grown from 1930 to 1999. Crop Sci. 44:1935–1946.
Smith, O.S., Smith, J.S.C. 1992. Measurement of genetic diversity among maize hybrids: a comparison of isozymic, RFLP, pedigree, and heterosis data. Maydica 37:53–80.
Souza Sobrinho, F., Ramalho, M.A.P., de Souza, J.C. 2001. Genetic diversity and inbreeding potential of maize commercial hybrids. Maydica 46:171–175.
Stojsin, D., Kannenberg, L.W., Rajnpreht, J., Pauls, P.K., Stojsin, R. 1996. Genetic relationships among commercial corn hybrids and parents based on RAPD analyses of pericarp and embryo DNA. Genetika 28:137–150.
Sun, G.L., William, M., Liu, J., Kasha, K.J., Pauls, K.P. 2001. Microsatellite and RAPD polymorphisms in Ontario corn hybrids are related to the commercial sources and maturity ratings. Molecular Breeding 7:13–24.
Troyer, A.F., Openshow, S.J., Knitle, K.H. 1983. Measurement of genetic diversity among commercial corn hybrids. Method. In: Agronomy abstracts, ASA, Madison, WI. p. 83.
Troyer, A.F., Openshow, S.J., Knitle, K.H. 1988. Measurement of genetic diversity among popular commercial corn hybrids. Crop Sci. 28:481–485.
Urquhart, S. 2000. Variance of binary data. Adapting a physical habitat protocol. https://doi.org/wwworegonstate.edu/instruct/str571/urquhart/phab/s1d019.htm
Williams, T.R., Hallauer, A.R. 2000. Genetic diversity among maize hybrids. Maydica 45:163–171.
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531–6535.
About this article
Cite this article
Bauer, I., Drinic, S.M., Drinić, G. et al. Assessing Temporal Changes in Genetic Diversity of Maize Hybrids Using RAPD Markers. CEREAL RESEARCH COMMUNICATIONS 35, 1563–1571 (2007). https://doi.org/10.1556/CRC.35.2007.4.3
- ZP hybrids
- genetic diversity