Barley (Hordeum vulgare L.) Marker Linkage Map: A Case Study of Various Marker Types and of Mapping Population Structure
A barley mapping population consisting of 96 doubled haploid lines of anther culture origin was developed from the varieties Dicktoo and Kompolti Korai, which represent diverse types with respect to geographical origin and ecological adaptation. Several molecular marker techniques were used in mapping: among the markers with known chromosome location, RFLP, STS and SSR markers were applied to identify linkage groups and for comparative mapping, while RAPD and AFLP markers, which have random binding but provide useful information on polymorphism, were employed to fill in the linkage groups with markers. A total of 496 markers were tested in the DH population, 246 of which were included in the linkage map after eliminating markers that were completely linked with each other. The ratio of markers with known chromosome location to random markers in the 1107 cM map was one to three, and the mean recombination distance between the markers was 4.5 cM. Application of various marker methods and the effect of the population structure on the development of marker linkage maps are discussed.
Keywordslinkage map molecular markers barley Hordeum vulgare L.
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- Karsai, I., Szűcs, P., Mészáros, K., Filichkina, T., Hayes, P.M., Skinner, J.S., Láng, L., Bedő, Z. 2005. The Vrn-H2 locus is a major determinant of flowering time in a facultative × winter growth habit barley (Hordeum vulgare L.) mapping population. Theor. Appl. Genet. 110:1458–1466.CrossRefGoogle Scholar
- Kleinhofs, A., Kilian, A., Saghai-Maroof, M.A., Biyashev, R.M., Hayes, P.M., Chen, F.Q., Lapitan, N., Fenwick, A., Blake, T.K., Kanazin, V., Ananiev, E., Dahleen, L., Kudrna, D., Bollinger, J., Knapp, S.J., Liu, B., Sorrells, M., Heun, M., Franckowiak, J.D., Hoffman, D., Skadsen, R., Steffenson, B.J. 1993. A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor. Appl. Genet. 86:705–712.CrossRefGoogle Scholar
- Linc, G., Karsai, I., Láng-Molnár, M., Bedő, Z. 1996. Comparison of RFLP probes and RAPD primers for studying genetic diversity in barley (Hordeum vulgare L.). Cereal Res. Comm. 24:283–290.Google Scholar
- Liu, Z.H., Anderson, J.A., Hu, J., Friesen, T.L., Rasmussen, J.B., Faris, J.D. 2005. A wheat intervarietal genetic linkage map based on microsatellite and target region amplified polymorphism markers and its utility for detecting quantitative trait loci. Theor. Appl. Genet. 111:782–794.CrossRefGoogle Scholar
- Mészáros, K., Karsai, I., Bedő, Z. 1996. Reliability of genetic distance estimation methods in barley (Hordeum vulgare L.). J. of Genetics & Breeding 50:387–392.Google Scholar
- Ramsay, L., Macaulay, M., Ivanissevich, S., MacLean, K., Cardle, L., Fuller, J., Edwards, K.J., Tuvesson, S., Morgante, M., Massari, A., Maestri, E., Marmiroli, N., Sjakste, T., Ganal, M., Powell, W., Waugh, R. 2000. A simple sequence repeat-based linkage map of barley. Genetics 156:1997–2005.PubMedPubMedCentralGoogle Scholar
- Rostoks, N., Mudie, S., Cardle, L., Russell, J., Ramsay, L., Booth, A., Svensson, J.T., Wanamaker, S.I., Waila, H., Rodrigez, E.M., Hedley, P.E., Liu, H., Morris, J., Close, T.J., Marshall, D.F., Waugh, R. 2005. Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Mol. Genet. Genom. 274:515–527.CrossRefGoogle Scholar
- Van Ooijen, J.W. 2006. JoinMap® 4, Software for the calculation of genetic linkage maps in experimental populations. Kyazma B.V. Wageningen, Netherlands.Google Scholar