Cereal Research Communications

, Volume 35, Issue 3, pp 1437–1448 | Cite as

Relationships Between Deoxynivalenol Content, Presence of Kernels Infected by Fusarium spp. Pathogens and Visually Scabby Kernels in Czech Wheat in 2003–2005

  • I. PolišenskáEmail author
  • L. Tvarůžek


The deoxynivalenol content, presence of kernels infected by Fusarium spp. pathogens and the frequency of visually scabby kernels in wheat intended for human consumption were monitored in 2003–2005. About 1000 samples provided by growers from various regions of the Czech Republic were analysed each year. Immunochemical assay ELISA-based kits for the analysis of deoxynivalenol content were employed. In 2003, 2004 and 2005, deoxynivalenol was detected at the maximum levels of 5090 µ−1, 18300 µ−1 and 4437 µ−1, respectively. Significant correlations were found between the deoxynivalenol content and percentage of kernels infected by Fusarium spp. pathogens, and percentage of visually scabby kernels. In all years under study, the relationship between the deoxynivalenol content and percentage of visually scabby kernels was closer than that between the deoxynivalenol content and percentage of kernels infected by Fusarium spp.


Fusarium winter wheat deoxynivalenol DON visually scabby kernels 


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  1. Abramson, D. 1998. Mycotoxin formation and environmental factors. In: Sinha, K.K., Bhatnagar, D. (eds), Mycotoxins in Agriculture and Food Safety. Marcel Dekker, New York, pp. 255–277.Google Scholar
  2. Amelung, D. 1996. Experiences with the isolation of plant pathogenic fungi. In: Proc. of the 4th Int. Sym. of the European Foundation for Plant Pathology: Diagnosis and identification of plant pathogens. Bonn, Germany, 09.–12.09. 1996, pp. 35–36.Google Scholar
  3. Atanasoff, Z., Nakamura, C., Mori, N., Kaneda, C. 1994. Mycotoxin production and pathogenicity of Fusarium species and wheat resistance to Fusarium head blight. Can. J. Bot. 72:161–167.CrossRefGoogle Scholar
  4. Chelkowski, J. 1998. Distribution of Fusarium species and their mycotoxins in cereal grains. In: Sinha, K.K., Bhatnagar, D. (eds), Mycotoxins in Agriculture and Food Safety. Marcel Dekker, New York, pp. 45–64.Google Scholar
  5. Clear, R.M., Patrick, S.K. 1990. Fusarium species isolated from wheat samples containing tombstones (scab) kernels from Ontario, Manitoba, and Saskatchewan. Can. J. Plant Sci. 70:1057–1069.CrossRefGoogle Scholar
  6. Cook, R.J. 1981. Fusarium diseases of cereals in Western Europe. In: Nelson, P.E., Toussoun, T.A., Cook, R.J. (eds), Fusarium: Diseases, Biology and Taxonomy. The Pensylvania State University Press, University Park and London, pp. 56–63.Google Scholar
  7. Dalcero, A., Torres, A., Etchevery, M., Chulze, S., Varsavsky, E. 1997. Occurrence of deoxynivalenol and Fusarium graminearum in Argentinian wheat. Food Additives and Contaminants 14:11–14.CrossRefGoogle Scholar
  8. Demcey Johnson, D., Flaskerud, G.K., Taylor, R.D., Satyanarayana, V. 1998. Economic impacts of fusarium head blight in wheat. Agricultural Economic Report no. 396. Agricultural Experiment Station, North Dakota State University: Department of Agricultural Economics.Google Scholar
  9. Dexter, J.E., Nowicki, T.W. 2003. Safety assurance and quality assurance issues associated with Fusarium head blight in wheat. In: Leonard, K.J., Bushnell, W.R. (eds), Fusarium Head Blight of Wheat and Barley. The American Phytopathological Society, St. Paul, MN, pp. 420–460.Google Scholar
  10. Eppley, R.M., Trucksess, M.W., Nesheim, S., Thorpe, C.W., Wood, G.E., Pohland, A.E. 1984. Deoxynivalenol in winter wheat: Thin layer chromatographic method and survey. J. Assoc. of Anal. Chem. 67:43–45.Google Scholar
  11. FAO 2002. Codex Alimentarius Commission, Joint FAO/WHO Food Standards Programme, Codex Committee on Food Additives and Contaminants, Thirty-fifth Session, Arusha, Tanzania, 17–21 March 2003: Discussion Paper on Deoxynivalenol. CX/FAC 03/35.Google Scholar
  12. Ittu, M., Grabarkiewicz-Szczesna, J., Kostecki, M., Golinski, P. 2000. Deoxynivalenol accumulation and other scab symptoms in six Romanian wheat genotypes inoculated with Fusarium graminearum. Mycotoxin Res. 16:15–22.CrossRefGoogle Scholar
  13. Joffé, A.Z. 1986. Fusarium Species: Their Biology and Toxicology. John Wiley and Sons, New York.Google Scholar
  14. Jones, R.K., Mirocha, C.J. 1999. Quality parameters in small grains from Minnesota affected by Fusarium head blight. Plant Diseases 83:506–511.CrossRefGoogle Scholar
  15. Liu, W., Langseth, W., Skinnes, H., Elen, O.N., Sundheim, L. 1997. Comparison of visual head blight ratings, seed infection levels and deoxynivalenol production for assessment of resistance in cereals inoculated with Fusarium culmorum. European Journal of Plant Pathology 103:589–595.CrossRefGoogle Scholar
  16. Logrieco, A., Bottalico, A. 2001. Distribution of toxigenic Fusarium species and mycotoxin associated with head blight of wheat in Europe. In: Proceedings of International Conference: Sustainable systems of cereal crop protection against fungal diseases as the way of reduction of toxin occurrence in the food webs. 02.–06.07. 2001, Kromeriz, pp. 83–89.Google Scholar
  17. Logrieco, A., Bottalico, A., Mul, G., Moretti, A., Perrone, G. 2003. Epidemiology of toxigenic fungi and their associated mycotoxins for some Mediterranean crops. European Journal of Plant Pathology 109:645–667.CrossRefGoogle Scholar
  18. Mathur, S.B., Cunfer, B.M. 1993. Seed-borne Diseases and Seed Health Testing of Wheat. Danish Gov. Inst. of Seed Pathol. for Develop. Countries, Copenhagen, pp. 83–93.Google Scholar
  19. Mesterházy, A. 2003. Breeding wheat for Fusarium head blight resistance in Europe. In: Leonard, K.J., Bushnell, W.R. (eds), Fusarium Head Blight of Wheat and Barley. The American Phytopathological Society, St. Paul, MN, USA, 312 pp.Google Scholar
  20. Mesterházy, A., Bartók, T. 1993. Resistance, pathogenicity and Fusarium spp. influencing toxin (DON) contamination of wheat varieties. Hodowla Rostlin, Akl. i Nas. 37:9–15.Google Scholar
  21. Miller, J.D., Young, J.C., Sampson, R.D. 1985. Deoxynivalenol and Fusarium head blight resistance in spring cereals. Phytopath. Z. 113:359–367.CrossRefGoogle Scholar
  22. Mirocha, C.J., Yu, H., Evans, C.K., Kolaczkowski, E.K., Dill-Macky, R. 1997. Chemistry and physiology of deoxynivalenol in pathogenesis. Cereal Res. Comm. 25: 309–314.Google Scholar
  23. Parry, D.W., Jenkinson, P., McLeod, L. 1995. Fusarium ear blight (scab) in small grain cereals — A review. Plant Pathology 44:207–238.CrossRefGoogle Scholar
  24. Perkowski, J., Chelkowski, J., Blazczak, P., Snijders, C.H.A., Wakulinski, W. 1991. A study of the correlation between the amount of deoxynivalenol in grain of wheat and triticale and percentage of Fusarium damaged kernels. Mycotoxin Res. 7A Part II: 102–114.CrossRefGoogle Scholar
  25. Schaafsma, A.W., Savard, M.E., Clear, R., Dexter, J. 2004. Methods and issues regarding detection of deoxynivalenol, Fusarium-damaged kernels, and Fusarium spp. in commercial grain in Canada. Can. J. Plant Pathol. 26:443–452.CrossRefGoogle Scholar
  26. Schnerr, H., Vogel, R.F., Niessen, L. 2002. Correlation between DNA of trichotecene-producing Fusarium species and deoxynivalenol concentrations in wheat samples. Letters in Applied Microbiology 35:121–125.CrossRefGoogle Scholar
  27. Sinha, R.C., Savard, M.E. 1997. Concentration of deoxynivalenol in single kernels and various tissues of wheat heads. Can. J. Plant. Pathol. 19:8–12.CrossRefGoogle Scholar
  28. Snijders, C.H.A., Perkowski, J. 1990. Effects of head blight caused by Fusarium culmorum on toxin content and weight of wheat kernels. Phytopathology 80:566–570.CrossRefGoogle Scholar
  29. Teich, A.H., Shugar, L., Smid, A. 1987. Soft white winter wheat cultivar field-resistance to scab and deoxynivalenol accumulation. Cereal Res. Comm. 15:109–114.Google Scholar
  30. Tkachuk, R., Dexter, J.E., Tipples, K.H., Nowicki, T.W. 1991. Removal by specific gravity table of tombstone kernels and associated trichothecenes from wheat infected with Fusarium head blight. Cereal Chemistry 68:428–431.Google Scholar
  31. Trendholm, H.L., Cochrane, W.P., Cohen, H., Elliot, J.I., Farnworth, E.R., Friend, D.W., Hamilton, R.M.G., Neish, G.A., Standish, J.F. 1981. Survey of vomitoxin contamination of the 1980 white winter wheat crop in Ontario, Canada. J. Am. Oil Chem. Soc. 58:992A–994A.CrossRefGoogle Scholar
  32. Trigo-Stockli, D.M., Curran, S.P., Pedersen, J.R. 1995. Distribution and occurrence of mycotoxins in 1993 Kansas wheat. Cereal Chem. 72:470–474.Google Scholar
  33. Tvarůžek, L., Ji, L., Cao, K. 2003. Reaction of winter wheat genotypes from Chinese and Czech collection to Fusarium head blight and leaf diseases. In Dagun Liu (ed.), Research of Plant Pathology in Hebei. Volume 1, China Agriculture Press, pp. 94–100.Google Scholar
  34. Visconti, A., Chelkowski, J., Botallico, A. 1986. Deoxynivalenol and 3-acetoxydeoxynivalenol-mycotoxins associated with wheat head fusariosis in Poland. Mycotoxin Res. 2:59–64.CrossRefGoogle Scholar
  35. Wiersma, J.W., Peters, E.L., Hanson, M.L., Bouvette, R.J., Bush, R.H. 1996. Fusarium head blight in hard red spring wheat: cultivar responses to natural epidemics. Agron. J. 88:223–230.CrossRefGoogle Scholar
  36. Wiese, M.V. 1987. Compendium of Wheat Diseases. 2nd edition, APS Press, St. Paul, MN, USA, 152 pp.Google Scholar
  37. Windels, C.E. 2000. Economic and social impact of Fusarium head blight: changing farm and rural communities in the northern Great Plains. Phytopathology 90:17–21.CrossRefGoogle Scholar
  38. Wong, L.S.L., Abramson, D., Tekauz, A., Leisle, D., McKenzie, R.I.H. 1995. Pathogenicity and mycotoxin production of Fusarium species causing head blight in wheat cultivars varying in resistance. Can. J. Plant Sci. 75:261–267.CrossRefGoogle Scholar
  39. Zhang, Z., Jin, Y. 2004. Investigation of kernel infection by Fusarium graminearum in wheat. Proceedings of the 2nd International Symposium on Fusarium Head Blight, Orlando, USA, 11–15 December 2004, pp. 540–542.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2007

Authors and Affiliations

  1. 1.Agrotest Fyto Ltd.KroměřížCzech Republic

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