Changes in Total- and α-amylase Activities and Wheat Germ Agglutinin Content in Wide-Range Herbicide Resistant Wheat Lines

Abstract

Protein sets, enzyme activities and immune reactivity against wheat germ agglutinin in the albumin-globulin fractions of parent and herbicide resistant transgenic wheat lines were studied.

Our results showed significantly increased amylase activities and increased immune reactivity against wheat germ agglutinin in the herbicide resistant transgenic wheat lines, investigated. The amylases and lectins belong to the plant food allergens; this explains why both scientists and consumers are interested in assessing the allergenic potential of plant proteins and the safety assessment of novel foods and GM foods in highlight of food safety. This paper is an important contribution to our database and the understanding of what is going on with genetic engineering of crop plants.

References

  1. Aalberse, R.C. 2000. Structural biology of allergens. Journal of Allergy and Clinical Immunology 106:228–238.

    CAS  Article  Google Scholar 

  2. Adessi, C., Miege, C., Albrieux, C., Rabilloud, T. 1997. Two-dimensional electrophoresis of membrane proteins: A current challenge for immobilized pH gradients. Electrophoresis 18:127–135.

    CAS  Article  Google Scholar 

  3. Alrefai, R. 2002. Biotechnology Consultation Note to the File BNF No. 000063, U. S., Food and Drug Administration, Center for Food Safety and Applied Nutrition.

  4. Barro, F., Barcelo, P., Lazzeri, P.A., Shewry, P.R., Martin, A., Ballesteros, J. 2002. Field evaluation and agronomic performance of transgenic wheat. Theoretical and Applied Genetics 105:980–984.

    CAS  Article  Google Scholar 

  5. Barro, F., Barcelo, P., Lazzeri, P.A., Shewry, P.R., Martin, A., Ballesteros, J. 2003. Functional properties and agronomic performance of transgenic Tritordeum expressing high molecular weight glutenin subunit genes 1Ax1 and 1Dx5. Journal of Cereal Science 37:65–70.

    CAS  Article  Google Scholar 

  6. Becker, D., Brettschneider, R., Lörz, H. 1994. Fertile transgenic wheat plants from microprojectile bombardment of scutellar tissue. Plant Journal 5:299–307.

    CAS  Article  Google Scholar 

  7. Christensen, A.H., Quail, P.H. 1996. Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monootyledonous plants. Transgenic Research 5:213–218.

    CAS  Article  Google Scholar 

  8. Decker, L.A. 1977. Worthington Enzyme Manual. Worthington Biochemical Corp., Freehold, NJ. pp. 173–176.

    Google Scholar 

  9. Felföldi, K., Purnhuaser, L. 1992.: Induction of regenerating callus from immature embryos of 44 wheat and 3 triticale cultivars. Cereal Res. Comm. 20:273–277.

    Google Scholar 

  10. Hajós, Gy., Gelencsér, É., Grant, G., Bardocz, S., Sakhri, M., Duguid, T.J., Newman, A.M., Pusztai, A. 1996. Effect of proteolytic modification and methionine enrichment on the nutritional value of soya albumins for rats. J. Nutritional Biochemistry 7:481–487.

    Article  Google Scholar 

  11. Horváth-Szanics, E., Szabó, Z., Janáky, T., Pauk, J., Hajós, Gy. 2006. Proteomics as an emergent tool for identification of stress-induced proteins in control and genetically modified wheat lines. Chromatographia 63:S143–S147.

    Article  Google Scholar 

  12. Krisch, J., Horváth, E., Vágvölgyi, Cs., Tanács, L. 2007. Mobility of herbicides and fungicides in soil and their effects on soil microorganism. Cereal Res. Comm. 35:673–676.

    CAS  Article  Google Scholar 

  13. Matucci, A., Veneri, G., Dalla Pellegrina, C., Zoccatelli, G., Vincenzi, S., Chignola, R., Peruffo, A.D.B., Rizzi, C. 2004. Temperature-dependent decay of wheat germ agglutinin activity and its implications for food processing and analysis. Food Control 15:391–395.

    CAS  Article  Google Scholar 

  14. Matuz, J., Póka, R., Boldizsár, I., Szerdahelyi, E., Hajós, Gy. 2000. Structure and potential allergenic character of cereal proteins. II. Potential allergens in cereal samples. Cereal Res. Comm. 28:433–442.

    CAS  Google Scholar 

  15. Mills, E.N.C., Madsen, C., Shewry, P.R., Wichers, H.J. 2003. Bubble formation and stabilization in bread dough. Trends in Food Science & Technology 14:145–156.

    CAS  Article  Google Scholar 

  16. Nehra, N.S., Chibbar, R.N., Leung, N., Caswell, K., Mallard, C., Steinhauser, L., Baga, M., Kartha, K.K. 1994. Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant Journal 5:285–297.

    CAS  Article  Google Scholar 

  17. Osborne, T.B. 1907. The Proteins of the Wheat Kernel. Carnegie Institute, Washington.

    Book  Google Scholar 

  18. O’Farrell, P.H. 1975. High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021.

    PubMed  Google Scholar 

  19. Pauk, J., Hänsch, R., Schwarz, G., Nerlich, A., Monostori, T., Mészáros, A., Jenes, B., Kertész, Z., Matuz, J., Schulze, J., Mendel, R.R. 1998. Transzgénikus búza (Triticum aestivum L.) előállítása Magyarországon [Genetic transformation of wheat (Triticum aestivum L.) in Hungary]. Növénytermelés 47:241–251.

    CAS  Google Scholar 

  20. Penaa, L.B., Pasquinia, L.A., Tomaroa, M.L., Callego, S.M. 2006. Proteolytic system in sunflower (Helianthus annuus L.) leaves under cadmium stress: Plant Science 171:531–537.

    Article  Google Scholar 

  21. Przymusiski, R., Ruciska, R., Gwóźdź, E.A. 2004. Increased accumulation of pathogenesis-related proteins in response of lupine roots to various abiotic stresses. Environmental and Experimental Botany 52:53–61.

    Article  Google Scholar 

  22. Pusztai, Á., Ewen, S.W.B., Grant, G., Brown, D.S., Stewart, J.C., Peumans, W.J., Van Damme, E.J.M., Bardocz, Zs. 1993. Antinutritive effects of wheat germ agglutinin and other N-acetylglucosamine-specific lectins. Br. J. Nutr. 70:313–321.

    CAS  Article  Google Scholar 

  23. Rakszegi, M., Tamás, C., Szűcs, P., Tamás, L., Bedő, Z. 2001. Current status of wheat transformation. Journal of Plant Biotechnology 3:67–81.

    Google Scholar 

  24. Rakszegi, M., Békés, F., Láng, L., Tamás, L., Shewry, P.R., Bedő, Z. 2005. Technological quality of transgenic wheat expressing an increased amount of a HMW glutenin subunit. Journal of Cereal Science 42:15–23.

    CAS  Article  Google Scholar 

  25. Van Damme, E.J.M., Peumans, W.J., Pusztai, Á., Bardocz, Zs. 1998. Handbook of plant lectins: properties and biomedical applications. John Wiley and Sons, West Sussex.

    Google Scholar 

  26. Van der Maarel, M.J.E.C., Van der Veen, B., Uitdehaag, J.C.M., Leemhuis, H., Dijkhuizen, L. 2002. Properties and applications of starch-converting enzymes of the α-amylase family. Journal of Biotechnology 94:137–155

    Article  Google Scholar 

  27. Vasil, V., Srivastava, V., Castillo, A.M., Fromm M., Vasil, I.K. 1993. Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. Bio/Technology 11:1553–1558.

    Google Scholar 

  28. Weeks, J.T., Anderson, O.D., Blechl, A.E. 1993. Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiology 102:1077–1084.

    CAS  Article  Google Scholar 

  29. Weil, J.H. 2005. Are genetically modified plants useful and safe? IUBMB Life 57:311–314.

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to J. Pauk.

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Cite this article

Halász, Á., Horváth-Szanics, E., Nagy-Gasztonyi, M. et al. Changes in Total- and α-amylase Activities and Wheat Germ Agglutinin Content in Wide-Range Herbicide Resistant Wheat Lines. CEREAL RESEARCH COMMUNICATIONS 35, 1405–1413 (2007). https://doi.org/10.1556/CRC.35.2007.3.5

Download citation

Keywords

  • transgenic wheat lines
  • WGA
  • amylase activity
  • food safety
  • 2D-electrophoresis
  • immunoblot