Changes in Total- and α-amylase Activities and Wheat Germ Agglutinin Content in Wide-Range Herbicide Resistant Wheat Lines
Protein sets, enzyme activities and immune reactivity against wheat germ agglutinin in the albumin-globulin fractions of parent and herbicide resistant transgenic wheat lines were studied.
Our results showed significantly increased amylase activities and increased immune reactivity against wheat germ agglutinin in the herbicide resistant transgenic wheat lines, investigated. The amylases and lectins belong to the plant food allergens; this explains why both scientists and consumers are interested in assessing the allergenic potential of plant proteins and the safety assessment of novel foods and GM foods in highlight of food safety. This paper is an important contribution to our database and the understanding of what is going on with genetic engineering of crop plants.
Keywordstransgenic wheat lines WGA amylase activity food safety 2D-electrophoresis immunoblot
- Alrefai, R. 2002. Biotechnology Consultation Note to the File BNF No. 000063, U. S., Food and Drug Administration, Center for Food Safety and Applied Nutrition.Google Scholar
- Decker, L.A. 1977. Worthington Enzyme Manual. Worthington Biochemical Corp., Freehold, NJ. pp. 173–176.Google Scholar
- Felföldi, K., Purnhuaser, L. 1992.: Induction of regenerating callus from immature embryos of 44 wheat and 3 triticale cultivars. Cereal Res. Comm. 20:273–277.Google Scholar
- Matuz, J., Póka, R., Boldizsár, I., Szerdahelyi, E., Hajós, Gy. 2000. Structure and potential allergenic character of cereal proteins. II. Potential allergens in cereal samples. Cereal Res. Comm. 28:433–442.Google Scholar
- Nehra, N.S., Chibbar, R.N., Leung, N., Caswell, K., Mallard, C., Steinhauser, L., Baga, M., Kartha, K.K. 1994. Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant Journal 5:285–297.CrossRefGoogle Scholar
- Pauk, J., Hänsch, R., Schwarz, G., Nerlich, A., Monostori, T., Mészáros, A., Jenes, B., Kertész, Z., Matuz, J., Schulze, J., Mendel, R.R. 1998. Transzgénikus búza (Triticum aestivum L.) előállítása Magyarországon [Genetic transformation of wheat (Triticum aestivum L.) in Hungary]. Növénytermelés 47:241–251.Google Scholar
- Rakszegi, M., Tamás, C., Szűcs, P., Tamás, L., Bedő, Z. 2001. Current status of wheat transformation. Journal of Plant Biotechnology 3:67–81.Google Scholar
- Van Damme, E.J.M., Peumans, W.J., Pusztai, Á., Bardocz, Zs. 1998. Handbook of plant lectins: properties and biomedical applications. John Wiley and Sons, West Sussex.Google Scholar
- Vasil, V., Srivastava, V., Castillo, A.M., Fromm M., Vasil, I.K. 1993. Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. Bio/Technology 11:1553–1558.Google Scholar
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.