Evaluation of Wheat Germplasm from the Madeira and Canary Archipelagos Using a Single Molecular Marker. A Rapid Screening Method for Identification of Durum Wheat Accessions


This work aims to present a rapid and precise screening method of wheat germplasm and identification of durum wheat accessions in germplasm collections. Fifty-two wheat accessions maintained in the ISOPlexis germplasm bank at the Madeira University, Portugal, and 72 accessions from the Centre for Conservation of Crop Biodiversity of Tenerife (CCBAT), Spain, have been screened for their specific ploidy status using the molecular marker Dgas44. We have demonstrated that the Dgas44 sequence is effective in the screening of Madeiran and Canarian wheat accessions. This screening method permitted the detection of 10 and 11 durum accessions among Madeiran and Canarian wheats, accounting for 19.2 and 15.3% of screened collections, respectively. The obtained results have shown a 100% of cases correspondence with the previously performed morphological identification of the Madeiran wheats. It also permitted rectification and clarification of previous classification of some accessions based only on the morphological traits. The PCR based assay was directly applicable to the screening of seeds and was suitable for detecting seed mixtures in accessions. This rapid method has been proven to be a useful tool in gene bank accessions management including verification of their ploidy status and detection of seed lots adulteration.


  1. Alary, R., Serin, A., Duviau, M.P., Joudrier, P., Gautier, M.F. 2002. Quantification of common wheat adulteration of durum wheat pasta using real-time quantitative polymerase chain reaction (PCR). Cereal Chem. 79:553–558.

    CAS  Article  Google Scholar 

  2. Ahmad, M. 2000. Molecular marker-assisted selection of HMW glutenin alleles related to wheat bread quality by PCR-generated DNA markers. Theor. Appl. Genet. 101:892–896.

    CAS  Article  Google Scholar 

  3. Bryan, G.J., Dixon, A., Gale, M.D., Wiseman, G. 1998. A PCR based method for the detection of hexaploide wheat adulteration of durum wheat and pasta. J. Cereal Sci. 28:135–145.

    CAS  Article  Google Scholar 

  4. Dellaporta, S.L., Word, J., Hicks, J.B. 1983. A plant DNA minipreparation: Version II. Plant Molecular Biology Report 1:19–22.

    CAS  Article  Google Scholar 

  5. Dograr, N., Akin-Yalin, S., Akkaya, M.S. 2000. Discriminating durum wheat cultivars using highly polymorphic simple sequence repeat DNA markers. Plant Breed. 119:360–362.

    CAS  Article  Google Scholar 

  6. Dos Santos, T.M.M., Pinheiro de Carvalho, M.A.A. 2006. Trigos da Macaronésia. Diversidade dos Trigos Regionais do Arquipélago da Madeira. Germobanco, Funchal, 122 pp.

    Google Scholar 

  7. Manifesto, M.M., Schlatter, A.R., Hopp, H.E., Suárez, E.Y., Dubcovsky, J. 2001. Quantitative Evaluation of genetic diversity in wheat germplasm using molecular markers. Crop Sci. 41:682–690.

    CAS  Article  Google Scholar 

  8. McNeil, D., Lagudah, E.S., Hohmann, U., Appels, R. 1994. Amplification of DNA sequences in wheat and its relatives: the Dgas44 and R350 families of repetitive sequences. Genome 37:320–327.

    CAS  Article  Google Scholar 

  9. Pasqualone, A., Lotti, C., Blanco, A. 1999. Identification of durum wheat cultivars and monovarietal semolinas by analysis of DNA microsatellites. Eur. Food Res. Technol. 210:144–147.

    CAS  Article  Google Scholar 

  10. Perry, D.J. 2004. Identification of Canadian durum wheat varieties using a single PCR. Theor. Appl. Genet. 109:55–61.

    CAS  Article  Google Scholar 

  11. Pinheiro de Carvalho, M.A.A., Slaski, J.J., Dos Santos, T.M.M., Ganança, F.T., Abreu, I., Taylor, G.J., Clemente Vieira, M.R., Popova, T.N., Franco, E. 2003. Identification of aluminium tolerant genotypes among Madeiran regional wheats. Communications in Soil Sciences and Plant Analysis 34 (19–20):2973–2985.

    Google Scholar 

  12. Tams, S.H., Bauer, E., Oettler, G., Melchinger, A.E. 2004. Genetic diversity in European winter triticale determined with SSR markers and coancestry coefficient. Theor. Appl. Genet. 108:1385–1391.

    CAS  Article  Google Scholar 

  13. Tilley, M. 2004. PCR amplification of wheat sequences from DNA extracted during milling and baking. Cereal Chem. 81:553–558.

    Article  Google Scholar 

  14. Vieira, A. 1983. O Comércio de cereais dos Açores para a Madeira no século XVII. Boletim do Instituto Histórico da Ilha Terceira. 16:651–677.

    Google Scholar 

Download references

Author information



Corresponding author

Correspondence to M. A. A. Pinheiro de Carvalho.

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Cite this article

Andrade, V., dos Santos, T.M.M., Morales, D.A. et al. Evaluation of Wheat Germplasm from the Madeira and Canary Archipelagos Using a Single Molecular Marker. A Rapid Screening Method for Identification of Durum Wheat Accessions. CEREAL RESEARCH COMMUNICATIONS 35, 1397–1404 (2007). https://doi.org/10.1556/CRC.35.2007.3.4

Download citation


  • durum wheat
  • germplasm
  • D genome
  • DNA
  • PCR
  • Dgas44