Relationship Between Hordein Proteins and Malt Quality in Barley Cultivars Grown in Croatia
The hordein proteins of ten barley (Hordeum vulgare L.) cultivars grown in region of eastern Croatia were studied for their contribution to the malting quality, particularly relating hordein influence on malt extract yield. The analysis of hordein proteins was carried out by reversed-phase high-performance liquid chromatography (RP-HPLC). The micromalting of investigated barley cultivars and malt analyses were done according to the EBC official methods. The quantitative analysis of hordein proteins has showed that B-hordein was major component, followed by C- and D-hordeins. Among investigated cultivars the malt extract yield varied from 76.8% (cv. Angora) to 82.6% (cv. Scarlett). Spring barleys had on the average higher malt extract yield in contrast to winter barleys. The significant negative correlation between the total hordein content and malt extract yield was found. Among hordein fractions the B-hordein and D-hordein significantly contributed to lower malt extract yield. RP-HPLC analysis of malt hordeins revealed that the amount of hordein degraded during malting significantly correlated with Kolbach index.
Keywordsmalting barley hordein RP-HPLC malt extract correlation protein degradation
- Baxter, E.D., Wainwright, T. 1979. Hordein and malting quality. J. Am. Soc. Brew. Chem. 37:8–12.Google Scholar
- European Brewery Convention Analysis Committee. 1998. Analytica-EBC. Verlag Hans Carl Getranke-Fachverlag, Nürnberg.Google Scholar
- Kreis, M., Shewry, P.R. 1992. The control of protein synthesis in developing barley seeds. In: Shewry, P.R. (ed.), Barley: Genetics, Biochemistry, Molecular Biology and Biotechnology. CAB International, Wallingford, pp. 319–334.Google Scholar
- Lalić, A., Kovačević, J., Novoselović, D., Babić, D., Gunjača, J. 2003. Ostvarena dobit i uèinak uvjeta uzgoja na urod zrna sorti ozimog i jarog ječma. In: Proceedings of the 38th Croatian Symposium on Agriculture with International Participation, Faculty of Agriculture, University of Zagreb, pp. 93–96.Google Scholar
- Marchylo, B.A., Kruger, J.E. 1984. Identification of Canadian barley cultivars by reversed-phase high performance liquid chromatography. Cereal Chem. 61:295–301.Google Scholar
- Marchylo, B.A., Hatcher, D.W., Kruger, J.E., Kirkland, J.J. 1992. Reversed-phase high performance liquid chromatographic analysis of wheat proteins using a new, highly stable column. Cereal Chem. 69:371–378.Google Scholar
- Marchylo, B.A., Kruger, J.E., Hatcher, D. 1986. High-performance liquid chromatographic and electrophoretic analysis of hordein during malting for two barley varieties of contrasting malting quality. Cereal Chem. 63:219–231.Google Scholar
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