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Cereal Research Communications

, Volume 35, Issue 1, pp 129–140 | Cite as

Protein Fractions in Barley Grains as Affected by Some Agronomic Factors and Their Relationships to Malt Quality

  • J. M. Wang
  • J. X. Chen
  • F. Dai
  • F. B. Wu
  • J. M. Yang
  • G. P. ZhangEmail author
Article

Abstract

The effects of sowing date, nitrogen application level and timing on barley protein components and malt quality were investigated. There was a significant difference in total protein and its protein fractions among the four barley genotypes. The protein component was changeable over the different growing conditions, and the extent of change varied with protein fraction and genotype. Marked variation in malt quality over the different environments (sowing date, N fertilizer rate and applying time) was also observed. Increased N fertilizer application increased diastatic power (DP) value, but reduced malt extract. Grain protein content was significantly and positively correlated with albumin, globulin and hordein, but was not correlated with glutelin. However, glutelin was significantly related to other malt quality parameters.

Keywords

barley (Hordeum vulgare L.) protein protein fractions malt quality 

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References

  1. Arends, A.M., Fox, G.P., Henry, R.J., Marschke, R.J., Symons, M.H. 1995. Genetic and environmental variation in the diastatic power of Australian barley. J. Cereal Sci. 21:63–70.CrossRefGoogle Scholar
  2. Bishop, L.R., Day, F.E. 1993. The effect of variety on the relation between nitrogen content and extract. J. Inst. Brew. 39:545–551.CrossRefGoogle Scholar
  3. Brennan, C.S., Smith, D.B., Harris, N., Shewry, P.R. 1998. The production and characterization of Hor3 null lines of barley provides new information on the relationship of D hordein to malting performance. J. Cereal Sci. 28:291–299.CrossRefGoogle Scholar
  4. Chen, J.X., Dai, F., Wei, K., Zhang, G.P. 2006. The effects of timing of nitrogen fertilizer application on some grain and malt qualities of barley. J. Zhejiang Univ. Sci. 7:79–84.CrossRefGoogle Scholar
  5. Chen, J.X., Zhang, G.P., Wang, J.M., Chen, Z.H., Zhou, T.Y. 2004. The effects of regime of n application on barley β-glucanase activity and malt quality. Acta Agron. Sinica 1:47–51.Google Scholar
  6. Coles, G.D., Jamieson, P.D., Haslemore, R.M. 1991. Effects of moisture stress on malting quality in Triumph barley. J. Cereal Sci. 14:161–177.CrossRefGoogle Scholar
  7. Duffus, C.M., Cochrane, M.P. 1993. Formation of the barley grain — Morphology, physiology and biochemistry. In: MacGregor, A.W., Bhatty, R.S. (eds), Barley: Chemistry and Technology, AACC, St. Paul, Minnesota, USA. pp. 31–72.Google Scholar
  8. Eagles, H.A., Bedgood, A.G., Panozzo, J.F., Martin, P.J. 1995. Cultivar and environmental effects on malting quality in barley. Aust. J. Agric. Res. 46:831–844.CrossRefGoogle Scholar
  9. European Brewery Convention. (1975) Analytica EBC, 3rd ed.Google Scholar
  10. Evans, E., van Wegen, B., Ma, Y.F., Eglinton, J. 2003. The impact of the thermostability of α-amylase, β-amylase, and limit dextrinase on potential wort fermentability. J. Am. Soc. Brew. Chem. 61:210–218.Google Scholar
  11. Georg-Kraemer, J.E., Mundstock, E.C., Cavalli-Molina, L. 2001. Developmental expression of amylase during barley malting. J. Cereal Sci. 33:279–288.CrossRefGoogle Scholar
  12. Giese, H., Hejgaard, J. 1984. Synthesis of salt-soluble proteins in barley. Pulse-labeling study of grain filling in liquid-cultured detached spikes. Planta 161:172–177.CrossRefGoogle Scholar
  13. Grant, C.A., Gauer, L.E., Gehl, D.T., Bailey, L.D. 1991. Protein production and nitrogen utilization by barley cultivars in response to nitrogen fertilization under varying moisture conditions. Can. J. Plant Sci. 71:997–1009.CrossRefGoogle Scholar
  14. Howard, K.A., Gayler, K.R., Eagles, H.A., Halloran, G.M. 1996. The relationship between D hordein and malting quality in barley. J. Cereal Sci. 24:47–53.CrossRefGoogle Scholar
  15. Lauriere, C., Lauriere, M., Daussant, J. 1986. Immuno-histochemical localization of β-amylase in resting barley seeds. Plant Physiol. 67:383–388.CrossRefGoogle Scholar
  16. Molina-Cano, J.L., Francesch, M., Perez-Vendrell, A.M., Ramo, T., Volttas, J., Brufau, J. 1997. Genetic and environmental variation in malting and feed quality of barley. J. Cereal Sci. 25:37–47.CrossRefGoogle Scholar
  17. Molina-Cano, J.L., Polo, J.P., Romera, E., Araus, J.L., Zarco, J., Swanston, J.S. 2001. Relationships between barley hordeins and malting quality in a mutant of cv. Triumph I. Genotype by environment interaction of hordein content. J. Cereal Sci. 34:285–294.CrossRefGoogle Scholar
  18. Molina-Cano, J.L., Ramo, T., Ellis, R.P., Swanston, J.S., Bain, H., Uribe-Echeverría, T., Pérez-Vendrell, A.M. 1995. Effect of grain composition on water uptake by malting barley: a genetic and environmental study. J. Inst. Brew. 101:79–83.CrossRefGoogle Scholar
  19. Molina-Cano, J.L., Rubió, A., Igartua, E., Gracia, P., Montoya, J.L. 2000. Mechanisms of malt extract development in barleys from different European regions. II. Effect of barley hordein fractions on malt extract yield. J. Inst. Brew. 106:117–123.CrossRefGoogle Scholar
  20. Osborne, T.B. 1924. The Vegetable Proteins. New York, Longmans Green and Co.Google Scholar
  21. Qi, J.C., Chen, J.X., Wang, J.M., Wu, F.B., Cao, L.P., Zhang, G.P. 2005. Protein and hordein fraction content in barley seeds as affected by sowing date and their relations to malting quality. J. Zhejiang Univ. Sci. (Life Science) 11:1069–1075.CrossRefGoogle Scholar
  22. Qi, J.C., Zhang, G.P., Zhou, M.X. 2006. Protein and hordein content in barley seeds as affected by nitrogen level and their relationship to beta-amylase activity. J. Cereal Sci. 43:102–107.CrossRefGoogle Scholar
  23. Rasmusson, D.C., Glass, R.L. 1965. Effectiveness of early generation selection for four quality characters in barley. Crop Sci. 5:389–361.CrossRefGoogle Scholar
  24. Shewry, P.R., Franklin, J., Parmar, S., Smith, S.J., Miflin, B.J. 1983. The effects of sulphur starvation on the amino acid and protein compositions of barley grain. J. Cereal Sci. 1:21–31.CrossRefGoogle Scholar
  25. Smith, D.B. 1990. Barley seed protein and its effects on malting and brewing quality. Plant Var. Seeds 3:63–80.Google Scholar
  26. Tang, Q.Y., Feng, M.G. 1997. Practiced Statistical Analysis and Data Processing System. China Agricultural Press, Beijing, pp. 67–76.Google Scholar
  27. Weston, D.T., Horsley, R., Schwarz, P.B., Goos, R.J. 1993. Nitrogen and planting date affect on low-protein spring barley. Agron. J. 85:1170–1174.CrossRefGoogle Scholar
  28. Yin, C., Zhang, G.P., Wang, J.M., Chen, J.X. 2002. Variation of beta-amylase activity in barley as affected by cultivar and environment and its relation to protein content and grain weight. J. Cereal Sci. 36:307–312.CrossRefGoogle Scholar
  29. Zhang, G.P., Chen, J.X., Dai, F., Wang, J.M., Wu, F.B. 2006. The effect of cultivar and environment on beta-amylase activity is associated with the change of protein content in barley grains. J. Agron. Crop Sci. 192:43–49.CrossRefGoogle Scholar
  30. Zhao, F.J., Fortune, S., Barbosa, V.L., McGrath, S.P., Stobart, R., Bilsborrow, P.E., Booth, E.J., Brown, A., Robson, P. 2006. Effects of sulphur on yield and malting quality of barley. J. Cereal Sci. 43:369–377.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2007

Authors and Affiliations

  • J. M. Wang
    • 1
    • 2
  • J. X. Chen
    • 1
  • F. Dai
    • 1
  • F. B. Wu
    • 1
  • J. M. Yang
    • 2
  • G. P. Zhang
    • 1
    Email author
  1. 1.Department of AgronomyZhejiang UniversityHangzhouP.R. China
  2. 2.Institute of Crop Science and Nuclear Technique ApplicationZhejiang Academy of Agricultural SciencesHangzhouP.R. China

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