Influence of Gluten Proteins on Technological Properties of Croatian Wheat Cultivars

Summary

The gluten proteins of 15 winter wheat cultivars grown in eastern Croatia were studied for their contribution to the bread-making quality. Composition of high-molecular-weight glutenin subunits (HMW-GS) was analyzed by SDS-PAGE, while the quantity of gluten proteins was determined by combined extraction/RP-HPLC procedure. The results of the linear correlation analysis carried out on the particular gluten proteins and technological properties showed that the amount of total gluten content highly correlates with protein content. Among gluten proteins, the glutenins showed higher correlation with protein content, with pronounced influence of HMW-GS, than gliadins. Wet gluten content was significantly correlated to total gliadin quantity. Gluten index as gluten quality parameter was positively influenced by total glutenins and low-molecular-weight glutenin subunits (LMW-GS), and negatively, by the ratios of gliadin to glutenin (Gli/Glu), whereas the amount of gliadins was not important. Dough development time was strongly correlated with total gluten content, total glutenins and the Gli/Glu ratio. Dough mixing resistance was strongly affected by total glutenin content with pronounced influence of HMW-GS. Degree of dough softening is mainly negative influenced by total glutenins and ratio of Gli/Glu. Farinograph quality number as flour quality index was highly positively correlated with total glutenins, with emphasized influence of HMW-GS. The Gli/Glu ratio had the highest influence on dough maximum resistance. Dough extensibility showed moderate correlation with total gliadins. The results of the linear correlation indicated that loaves volumes were significantly influenced by total gluten proteins, HMW-GS and LMW-GS.

References

  1. Antes, S., Wieser, H. (2001) Effects of high and low molecular weight glutenin subunits on rheological dough properties and breadmaking quality of wheat. Cereal Chem. 78, 157–159

    CAS  Article  Google Scholar 

  2. Daniel, C., Triboi, E. (2000) Effect of temperature and nitrogen nutrition on the grain composition of winter wheat. Effects on gliadin content and composition. J. Cereal Sci. 32, 45–56

    CAS  Article  Google Scholar 

  3. Horvat, D., Jurković, Z., Sudar, R., Pavlinić, D., Šimić, G. (2002) The Relative Amounts of HMW Glutenin Subunits of OS Wheat Cultivars in Relation to Bread-Making Quality. Cereal Res. Comm. 30, 415–422

    CAS  Google Scholar 

  4. Ivanov, P., Todorov, I., Stoeva, I., Ivanova, I. (1998) Storage proteins characterisation of group of new Bulgarian high breadmaking quality wheat lines. Cereal Res. Comm. 26, 447–454

    CAS  Google Scholar 

  5. Johansson, E., Nillson, H., Mazhar, H., Skerritt, J., Macritchie, F., Svensson, G. (2002) Seasonal effects on storage proteins and gluten strength in four Swedish wheat cultivars. J. Sci. Food Agr. 82, 1305–1311

    CAS  Article  Google Scholar 

  6. Johansson, E., Prieto-Linde, M.L., Svensson, G. (2004) Influence of nitrogen application rate and timing on grain protein composition and gluten strength in Swedish wheat. J. Plant Nutr. Soil Sci. 167, 345–350

    Article  Google Scholar 

  7. Jood, S., Schofield, J.D., Tsiami, A.A., Bollecker, S. (2001) Effect of glutenin subfractions on bread-making quality of wheat. Internat. J. Food Sci. & Tech. 36, 573–584

    CAS  Article  Google Scholar 

  8. Jurković, Z., Sudar, R., Drezner, G., Horvat, D. (2000) The HMW Glutenin Subunit Composition of OS Wheat Cultivars and their Relationship with Bread-Making Quality. Cereal Res. Comm. 28, 271–277

    Google Scholar 

  9. Kuktaite, R., Johansson, E., Juodeikiene, G. (2000) Composition and concentration of proteins in Lithuanian wheat cultivars: relationships with bread-making quality. Cereal Res. Comm. 288, 195–202

    Google Scholar 

  10. Lasztity, R. (2003) Prediction of Wheat Quality-Succes and Doubts. Periodica politechnica Ser. Chem. Eng. 46, 39–49

    Google Scholar 

  11. Payne, P.I., Lawrence, G.J., (1983) Catalogue of allels for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high-molecular weight subunits of gluten in hexaploid wheat. Cereal Res. Comm. 11, 29–35

    Google Scholar 

  12. Pechanek, U., Karger, A., Gröger, S., Charvat, B., Schöggl, G., Lelley, T. (1997) Effect of Nitrogen Fertilization on Quantity of Flour Protein Components, Dough Properties and Breadmaking quality of Wheat. Cereal Chem. 74, 800–805

    CAS  Article  Google Scholar 

  13. Uthayakumaran, S., Stoddard, F.L., Gras, P.W., Bekes, F. (2000) Effect of incorporated Glutenins on Functional Properties of Wheat Dough. Cereal Chem. 77, 737–743

    CAS  Article  Google Scholar 

  14. Wieser, H., Antes, S., Selmeier, W. (1998) Quantitative determination of gluten protein types in wheat flour by reverse-phase high-performance liquid chromatography. Cereal Chem 75, 644–650

    CAS  Article  Google Scholar 

  15. Wieser, H., Kieffer, R. (2001) Correlations of the amount of gluten protein types to the technological properties of wheat flours determined on a micro-scale. J. Cereal Sci. 34, 19–27

    CAS  Article  Google Scholar 

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Correspondence to D. Horvat.

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Horvat, D., Jurković, Z., Drezner, G. et al. Influence of Gluten Proteins on Technological Properties of Croatian Wheat Cultivars. CEREAL RESEARCH COMMUNICATIONS 34, 1177–1184 (2006). https://doi.org/10.1556/CRC.34.2006.2-3.258

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Key words

  • wheat
  • technological properties
  • gluten proteins
  • RP-HPLC
  • quantification
  • correlation