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Cereal Research Communications

, Volume 34, Issue 2–3, pp 879–886 | Cite as

DNA methylation polymorphism in a set of elite maize inbred lines revealed by methylation-sensitive ISSR analysis

  • Xinxin Zhao
  • Enji Jia
  • Weiguang Yang
  • Yingshan Dong
  • Bao LiuEmail author
Article

Summary

DNA methylation polymorphism among nine elite maize inbred lines was assessed by inter-simple sequence repeat (ISSR) fingerprinting on intact DNA and DNA digested by either of a pair of methylation-sensitive isoschizomers, Hpa II and Msp I. It was found that, along with distinct genetic differentiation, extensive DNA methylation polymorphism exists among the nine inbred lines. The line-specific methylation patterns are homogeneous within each line, indicating their usefulness as molecular markers for cultivar identification. DNA sequences underlying the DNA methylation variations include a high proportion of coding genes. Cluster analysis, however, indicates the existence of incongruence between DNA methylation polymorphism and known-pedigree of the maize inbred lines.

Index Words

Maize inbred lines DNA methylation polymorphism ISSR fingerprinting Molecular marker 

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Copyright information

© Akadémiai Kiadó, Budapest 2006

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Xinxin Zhao
    • 1
    • 2
  • Enji Jia
    • 2
  • Weiguang Yang
    • 2
  • Yingshan Dong
    • 3
  • Bao Liu
    • 1
    Email author
  1. 1.Laboratory of Molecular Epigenetics, Institute of Genetics & CytologyNortheast Normal UniversityChangchunChina
  2. 2.Department of AgronomyJilin Agricultural UniversityChangchunChina
  3. 3.The National Center of Transgenic Plant Research & CommercializationGongzulingChina

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