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Cereal Research Communications

, Volume 34, Issue 2–3, pp 871–878 | Cite as

AFLP and SSR fingerprinting to evaluate genetic diversity among bread wheat cultivars in Tunisia

  • Khaled MasmoudiEmail author
  • Ahmed Rebai
  • Radhouane Ellouz
Article
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Summary

Bread wheat (Triticum aestivum L.) is an economically and nutritionally important cereal crop in the Mediterranean region. Characterization of wheat germplasm by means of DNA fingerprinting techniques provides a tool to assess genetic diversity and to identify varieties. In this study, six Tunisian bread wheat cultivars were characterized by AFLP and SSR markers. Five AFLP primer pairs showed clear different patterns and seems to be the most suitable for analysis of the bread wheat varieties. Three SSR primers were polymorphic with more than two alleles. The pairwise genetic similarities (GS) based on these molecular markers were calculated and used to construct a dendrogram that allowed the discrimination of the six cultivars. The GS among the six varieties ranged from 0.79 to 0.36. The six varieties used in this work clustered into four groups using either AFLP or SSR markers. A high GS was found between Tebecca and Vaga Varieties which have a similar pedigree.

Keywords

Amplified fragment length polymorphism microsatellites SSR genetic markers wheat (Triticum aestivumgenetic similarity 

Nomenclature

AFLP

amplified fragment length polymorphism

CTAB

hexadecyltrimethylammonium bromide

SSR

simple sequence repeat

TBE

Tris-borateEDTA

TE

Tris-EDTA

CIMMYT

International Maize and Wheat Improvement Center

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Copyright information

© Akadémiai Kiadó, Budapest 2006

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Khaled Masmoudi
    • 1
    Email author
  • Ahmed Rebai
    • 1
  • Radhouane Ellouz
    • 1
  1. 1.Plant Molecular Genetics UnitCentre of Biotechnology of Sfax (CBS), B.P’K’SfaxTunisia

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