Demonstration of Aegilops biuncialis chromosomes in a wheat background by genomic in situ hybridization (GISH) and identification of U chromosomes by FISH using GAA sequences
The aims of the study were the optimisation of genomic in situ hybridization (GISH) and the use of a GAA satellite sequences as a fluorescence in situ hybridization (FISH) probe to discriminate Aegilops biuncialis and wheat chromosomes. The application of genomic DNA from the diploid progenitors, Ae. umbellulata and Ae. comosa, as a probe with a blocking ratio of 1:200 resulted in the clear visualization of Ae. biuncialis chromosomes. Based on the strong hybridization signals of the PCR-amplified GAA satellite sequences it was possible to identify chromosomes 1U, 2U, 4U and 5U of Ac. umbellulata. The improved GISH and GAA idiogram presented here allow the Ae. biuncialis chromatin to be traced in more detail during chromosome-mediated gene transfer to wheat.
Key wordsAegilops biuncialis Aegilops umbellulata GISH FISH GAA banding
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- Gill, B. S. (1987) Chromosome banding methods, standard chromosome band nomenclature, and applications in cytogenetic analysis. In Wheat and Wheat Improvement. Edited by Heyne, E. G. American Society of Agronomy, Madison, Wis. pp. 243–254.Google Scholar
- Logojan, A. A. and Molnár-Láng, M. (2000) Production of Triticum aestivum — Aegilops biuncialis chromosome additions. Cereal Research Communications, 28, 221–228.Google Scholar
- Molnár, I., Gáspár, L., Sárvári, E., Dulai, S., Hoffmann, B., Molnár-Láng, M. and Galiba, G. (2004) Physiological and morphological responses to water stress in Aegilops biuncialis and Triticum aestivum genotypes with differing tolerance to drought. Functional Plant Biology, 31, 1149–1159.CrossRefGoogle Scholar
- Schneider A., Line, G, Molnár, I. and Molnár-Láng, M. (2004) Molecular cytogenetic characterization of Aegilops biuncialis and its use for the identification of five derived wheal/Aegilops biuncialis disomic addition lines. Submitted to GenomeGoogle Scholar
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